Regulatory Mechanisms in Autoimmune Arthritis

自身免疫性关节炎的调节机制

• 批准号: 8597917
• 负责人: DAVID D. BRAND
• 金额: --
• 依托单位: MEMPHIS VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-10-01 至 2016-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8597917
• 关键词: Address; Affect; Animal Model; Antigens; Arthritis; Autoimmune Diseases; Autoimmune Process; Autoimmune Responses; Autoimmunity; Cartilage; Cells; Characteristics; Clinic; Collagen; Collagen Arthritis; Complement Factor B; Complex; Development; Disease; Disease remission; Employee Strikes; Etiology; Exposure to; Green Fluorescent Proteins; Homeostasis; Human; Immune; Immune response; Immune system; In Vitro; Inflammation; Inflammation Mediators; Inflammatory; Interleukin-17; Interleukin-6; Interruption; Joints; Label; Ligands; Measures; Mediating; Modality; Modeling; Mus; Nature; Participant; Pathogenesis; Pathologic; Pathology; Pathway interactions; Patients; Phenotype; Play; Population; Predisposition; Prevention; Process; Quality of life; Reaction; Regulation; Regulatory T-Lymphocyte; Reporter; Research; Research Project Grants; Resistance; Rheumatoid Arthritis; Role; Severities; Signal Transduction; Synovial Fluid; System; T-Lymphocyte; Therapeutic; Transforming Growth Factors; Transgenic Organisms; Translating; Veterans; Work; autoimmune arthritis; base; bone; cell type; design; human leukocyte antigen gene; immunoregulation; in vivo; in vivo Model; interest; joint function; joint injury; lymph nodes; novel therapeutic intervention; precursor cell; prevent; promoter; red fluorescent protein; response; tool

DESCRIPTION (provided by applicant): Rheumatoid arthritis (RA) is a crippling disease afflicting millions of people worldwide. Its progressive, destructive pathology significantly affects mobility and quality of life. RA is a complex disease process that involves the interaction of HLA molecules and immune cells that results in synovial inflammation, cartilage and bone destruction, and loss of joint function. Although its etiology is still a mystery, it is clear that there is a strong association between susceptibility to RA and the HLA genes that mediate immune responses, suggesting an autoimmune basis for the disease. Regulatory T cells (Tregs) are a newly described subset of suppressive cells that are absolutely critical for immune homeostasis. Without a proper compliment of Tregs, multiple forms of autoimmunity develop quickly. It is clear that Tregs can modulate autoimmunity, but there are large gaps in our understanding of how Tregs function in vivo, and how they can be manipulated for the development of new therapeutic approaches to the treatment of autoimmune diseases. We will use a Foxp3gfp/IL-17Frfp dual reporter readout in the context of our well- characterized animal model for arthritis to give us a unique opportunit to dissect the role of Treg cells in autoimmunity, allowing us to directly examine the ability of these cells to regulate an autoimmune response and we can directly measure how these manipulations can prevent disease pathology. Our research to date has suggested that parenteral exposure of the vertebrate immune system to a soluble antigen supplied without co-stimulation can result in an activation and expansion of a pre-existing population of regulatory T cells. It is our hypothesis that this activation provides the commitment necessary to prevent the possible conversion of these nTregs into Th17 cells upon subsequent exposure to their cognate ligand under pro-inflammatory conditions such as those found in the rheumatoid joint. We propose to use both in vitro and in vivo models to determine the mechanism(s) by which parenteral antigen induced immunoregulation prevents the development of the Th17 cell response, thereby providing protection from the debilitating joint pathologies characteristic of RA. We will also determine if the proper stimulation of Tregs can prevent them from converting to Th17 cells in the presence of pro-inflammatory mediators. Successful elucidation of the answers to these problems will allow us to design cell-based therapeutic treatments for those veterans suffering from the debilitating effects of RA and other inflammatory autoimmune diseases.

描述（由申请人提供）：