Genetic Resource for Optical Signaling
光信号遗传资源
基本信息
- 批准号:8898204
- 负责人:
- 金额:$ 64.34万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-08-01 至 2019-04-30
- 项目状态:已结题
- 来源:
- 关键词:1,2-diacylglycerolAdoptionAffinityAlarAreaBacterial Artificial ChromosomesBiological ProcessBiologyBiosensorCardiopulmonaryCardiovascular DiseasesCardiovascular systemCellsCommunitiesComplexCoupledCouplingCyclic AMPDiglyceridesDiseaseEndothelial CellsEndotheliumEngineeringEventFundingGene Transfer TechniquesGenerationsGeneticGrantHeart DiseasesHematological DiseaseImageInvestigationKnowledgeLaboratoriesLightLung diseasesMammalsMediatingMethodsMolecularMouse ProteinMouse StrainsMusMuscle TonusMutant Strains MiceMyocardial InfarctionMyocardiumNational Heart, Lung, and Blood InstituteOpticsPhysiologicalPrincipal InvestigatorProductionProteinsPulmonary Heart DiseasePulsarReagentResearchResearch PersonnelResourcesScienceScientistSecond Messenger SystemsSignal TransductionSmooth MuscleSmooth Muscle MyocytesSpecific qualifier valueTechnologyTimeTissuesTransgenesTransgenic MiceVascular DiseasesVasodilationVirus Diseasesabstractingcalcium indicatorcombinatorialcost effectivedesigndetectorembryonic stem cellexperiencefluorescence imaginggenetic resourcein vivoinduced pluripotent stem cellinterestneural circuitoptical sensoroptogeneticspromoterreceptorresearch studysecond messengersensortooltransgene expression
项目摘要
DESCRIPTION (provided by applicant): Advances in genetically encoded sensor and effector proteins have introduced unprecedented opportunities for the study of complex biological processes in vivo that provide an opportunity to rapidly advance knowledge critical to the understanding and treatment of cardiovascular and cardiopulmonary diseases. These optical tools have not been widely exploited due to the lack of available transgenic mouse lines expressing these reagents in relevant tissues. Such genetic reagents could be easily and efficiently deployed for the study of NHLBI relevant biology and disease, transforming the capacity of NHLBI scientists. We propose the creation of purpose -designed mouse lines that express Ca2+ sensors and Optogenetic cell activators in NHLBI relevant tissues. The Genetic Resource for Optical Signaling will consist of 50 mouse lines designed for combinatorial crosses enabling the co-expression of sensors and effectors, or red and green Ca2+ sensors in interacting lineages. This strategy will enable the combination of two synergistic technologies (Ca2+ sensors and optogenetic proteins) through simple, high-yield crosses that constrain expression of the transgene to cells of interest. As such, the resource will enable NHLBI researchers to conduct experiments not otherwise possible in a cost effective and highly efficient manner, accelerating the use of optical genetic tools and markedly accelerating scientific discovery. The Genetic Resource for Optical Signaling (GROS) will create and validate the lines, communicate their availability, and permanently bank them for distribution to the scientific community following the funding period.
RELEVANCE: We will create a resource that advances cardiovascular and cardiopulmonary science consisting of lines of mice expressing 3rd generation optical sensors and cell activators in lineages of relevance to NHLBI science. These mice will be fully characterized and made available to the community, creating permanently available reagents that will markedly enhance the capabilities of hundreds of scientists. (End of Abstract)
描述(由申请人提供):基因编码的传感器和效应蛋白的进展为研究体内复杂的生物过程带来了前所未有的机会,为快速推进对理解和治疗心血管和心肺疾病至关重要的知识提供了机会。由于缺乏在相关组织中表达这些试剂的可用转基因小鼠品系,这些光学工具尚未被广泛利用。这种基因试剂可以很容易和有效地用于NHLBI相关生物学和疾病的研究,改变NHLBI科学家的能力。我们建议建立专门设计的小鼠品系,在NHLBI相关组织中表达Ca2+传感器和光遗传细胞激活剂。光信号遗传资源将由50个小鼠品系组成,这些小鼠品系被设计用于组合杂交,使得传感器和效应器或红色和绿色Ca2+传感器在相互作用的谱系中共表达。该策略将使两种协同技术(Ca2+传感器和光遗传学蛋白)能够通过简单、高产的杂交结合,所述杂交将转基因的表达限制在感兴趣的细胞中。因此,该资源将使NHLBI的研究人员能够以具有成本效益和高效的方式进行实验,加速光学遗传工具的使用,并显着加快科学发现。光信号遗传资源(GROS)将创建和验证这些线,传达它们的可用性,并永久保存它们,以便在资助期后分发给科学界。
相关性:我们将创建一个促进心血管和心肺科学的资源,包括表达与NHLBI科学相关的第三代光学传感器和细胞激活剂的小鼠品系。这些小鼠将被充分表征并提供给社区,创造永久可用的试剂,这将显着提高数百名科学家的能力。(End摘要)
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Michael I. Kotlikoff其他文献
Junctional Cleft [Ca]<sub>i</sub> Measurements using Novel Cleft-Targeted Ca Sensors
- DOI:
10.1016/j.bpj.2011.11.2230 - 发表时间:
2012-01-31 - 期刊:
- 影响因子:
- 作者:
Sanda Despa;Julie Bossuyt;Bo Shui;Michael I. Kotlikoff;Donald M. Bers - 通讯作者:
Donald M. Bers
The Vena Cava Is Pacing The Embryonic Heart
- DOI:
10.1016/j.bpj.2008.12.3686 - 发表时间:
2009-02-01 - 期刊:
- 影响因子:
- 作者:
Philipp Sasse;Bernd K. Fleischmann;Michael I. Kotlikoff;Yvonne N. Tallini - 通讯作者:
Yvonne N. Tallini
Michael I. Kotlikoff的其他文献
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{{ truncateString('Michael I. Kotlikoff', 18)}}的其他基金
Vascular Precursors and Cell-Cell Signaling in Heart Vasculogenesis
心脏血管发生中的血管前体和细胞间信号传导
- 批准号:
9249631 - 财政年份:2015
- 资助金额:
$ 64.34万 - 项目类别:
Vascular Precursors and Cell-Cell Signaling in Heart Vasculogenesis
心脏血管发生中的血管前体和细胞间信号传导
- 批准号:
8864615 - 财政年份:2015
- 资助金额:
$ 64.34万 - 项目类别:
Design of Genetically Encoded Ca2+ Indicators for in Vivo Application
用于体内应用的基因编码 Ca2 指示剂的设计
- 批准号:
7933652 - 财政年份:2009
- 资助金额:
$ 64.34万 - 项目类别:
RNA Aptamers to Green Fluorescent Protein for Cell Imaging
用于细胞成像的绿色荧光蛋白 RNA 适体
- 批准号:
7318372 - 财政年份:2007
- 资助金额:
$ 64.34万 - 项目类别:
In Vivo Ca2+ and Voltage Imaging on The Urinary Bladder
膀胱体内 Ca2 和电压成像
- 批准号:
7197712 - 财政年份:2007
- 资助金额:
$ 64.34万 - 项目类别:
In Vivo Ca2+ and Voltage Imaging on The Urinary Bladder
膀胱体内 Ca2 和电压成像
- 批准号:
7346959 - 财政年份:2007
- 资助金额:
$ 64.34万 - 项目类别:
RNA Aptamers to Green Fluorescent Protein for Cell Imaging
用于细胞成像的绿色荧光蛋白 RNA 适体
- 批准号:
7465429 - 财政年份:2007
- 资助金额:
$ 64.34万 - 项目类别:
In Vivo Ca2+ and Voltage Imaging on The Urinary Bladder
膀胱体内 Ca2 和电压成像
- 批准号:
7618459 - 财政年份:2007
- 资助金额:
$ 64.34万 - 项目类别:
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