Synaptic FGFs are required and sufficient to maintain and repair aged NMJs

突触 FGF 是维持和修复老化 NMJ 所必需的且足够的

• 批准号: 9143829
• 负责人: Gregorio Valdez
• 金额: $ 37.68万
• 依托单位: VIRGINIA POLYTECHNIC INST AND ST UNIV
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2017-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9143829
• 关键词: Adult; Affect; Age; Aging; Alzheimer' s Disease; Attenuated; Axon; Binding; Binding Proteins; Biological; Brain; Cachexia; Caring; Cell Nucleus; Data; Development; Disease; Exhibits; Extracellular Matrix; Fibroblast Growth Factor; Fibroblast Growth Factor 1; Genes; Goals; Government; Health; Individual; Injury; Knockout Mice; Lead; Maintenance; Masks; Medical; Molecular; Molecular Target; Motor; Motor Neurons; Mus; Muscle; Muscle Fibers; Muscle function; Muscular Atrophy; Muscular Dystrophies; Mutation; Nerve; Nerve Endings; Neuromuscular Junction; Peripheral Nerves; Predisposition; Premature aging syndrome; Presynaptic Terminals; Quality of life; Research; Resources; Role; Signal Pathway; Signal Transduction; Skeletal Muscle; Synapses; System; Testing; Therapeutic Intervention; Work; age related; aged; axonal degeneration; base; keratinocyte growth factor; member; motor deficit; muscle aging; neuromuscular system; normal aging; novel; prevent; receptor; reinnervation; repaired; research study; therapeutic target; tool; tool development; transcriptome sequencing; wasting; young adult

 DESCRIPTION (provided by applicant): Motor function declines with aging resulting in impaired mobility, loss of independence and increased susceptibility to injury and diseases. Many studies have sought to determine the cellular and molecular factors that contribute to aging of the neuromuscular system. Recent findings strongly suggest that structural and molecular alterations at neuromuscular junctions occur before motor neurons and skeletal muscle fibers exhibit obvious age-related pathological changes. Thus, deleterious changes at the neuromuscular junction may trigger the slow but consistent loss of motor function during aging. While it is known that muscle-derived factors are required to maintain and repair the neuromuscular junction, the identity of such factors has remained elusive. To this end, we have taken varied, yet complementary, approaches to identify muscle- derived factors that function to maintain the structural and functional integrity of neuromuscular junctions from the ravages of aging. In this regard, we have gathered preliminary data suggesting that three members of the fibroblast growth factor (FGF) signaling pathway, FGF-7/10/22, and a FGF-binding protein (FGFBP1) are promising candidate molecules for protecting neuromuscular junctions from insults emanating from normal aging, ALS-causing mutations and injury to peripheral nerves. In mice, deletion of FGF-22 results in premature aging of the neuromuscular junction. It also delays reinnervation of skeletal muscles after injury. Similarly, a reduction in FGFBP1 accelerates age-associated changes at neuromuscular junctions and compromises motor function in young adult mice. Importantly, introducing FGFBP1 and FGF-22 into denervated muscles accelerates their reinnervation, further indicating that FGF-22 and FGFBP1 function to repair the neuromuscular junction. We strongly believe that the proposed experiments could lead to new molecular targets for developing therapeutic interventions to protect and repair the neuromuscular junction, and thus slow, prevent or even reverse aging of the motor system.

 描述（由申请人提供）：运动功能随着年龄的增长而下降，导致活动能力受损，丧失独立性，对损伤和疾病的易感性增加。许多研究试图确定导致神经肌肉系统老化的细胞和分子因素。最近的研究结果强烈地表明，在运动神经元和骨骼肌纤维表现出明显的与年龄相关的病理变化之前，神经肌肉接头的结构和分子改变就已经发生。因此，在神经肌肉接头的有害变化可能会触发缓慢但一致的运动功能在老化过程中的损失。虽然已知肌肉衍生因子是维持和修复神经肌肉接头所必需的，但这些因子的身份仍然难以捉摸。为此，我们已经采取了多种但互补的方法来鉴定肌肉源性因子，其功能是维持神经肌肉接头的结构和功能完整性免受衰老的破坏。在这方面，我们已经收集了初步的数据表明，成纤维细胞生长因子（FGF）信号通路的三个成员，FGF-7/10/22，和FGF结合蛋白（FGFBP 1）是有前途的候选分子，用于保护神经肌肉接头免受正常老化，ALS引起的突变和周围神经损伤的损害。在小鼠中，FGF-22的缺失导致神经肌肉接头的过早老化。它还延迟了骨骼肌损伤后的神经再支配。类似地，FGFBP 1的减少加速了神经肌肉接头的年龄相关变化，并损害了年轻成年小鼠的运动功能。重要的是，将FGFBP 1和FGF-22引入失神经肌肉加速了它们的神经再支配，进一步表明FGF-22和FGFBP 1具有修复神经肌肉接头的功能。我们坚信，拟议的实验可能会导致新的分子靶点，用于开发治疗干预措施，以保护和修复神经肌肉接头，从而减缓，预防甚至逆转运动系统的老化。