Small Silencing RNA Function in Genome Maintenance and Gamete Development

核心摘要 生物信息学核心将作为数据交换中心，提供标准化质量 为这笔赠款的所有四个项目提供保证、序列数据处理和可视化。通过 这样做将确保兼容和一致的数据格式、参数优化 和集中存储。现有的UMMS生物信息管道将进行优化，以 提供并标准化阅读对齐、对齐处理(高峰呼叫、文字记录 组装、小RNA作图等、富集化分析(基因本体富集化， 等)，以及数据集集成(例如，在不同的 条件)。因此，项目数据的集中存储、处理和分析 显著增强所有四个项目组成部分之间的集成。 相关性 生物信息学核心(核心C)提供的服务对研究是必不可少的 由所有四个项目提出。核心将提供存储、质量控制和 三个实验项目(I-III)高通量数据的初步分析， 并将这些数据提供给计算项目以供深入研究(项目四)。

Core Summary The bioinformatics core will serve as a data clearinghouse, offering standardized quality assurance, sequence data handling and visualization to all four projects of this grant. By doing so it will ensure compatible and consistent data formats, parameter optimization and centralized storage. Existing UMMS bioinformatics pipelines will be optimized to provide and standardize read alignment, alignment processing (peak calling, transcript assembly, small RNA mapping, etc., enrichment analysis (Gene Ontology enrichments, etc.), and dataset integration (e.g. differential expression of piRNAs in different conditions). The centralized storage, processing and analysis of project data will thus significantly enhance integration between all four project components. Relevance The services provided by the Bioinformatics Core (Core C) are essential to research proposed by all four Projects. The core will provide storage, quality control, and preliminary analysis of high-throughput data from the three experimental projects (I-III), and make this data available to the computational project for in depth study (Project IV).