DNA Sequencing Using Single Molecule Electronics

使用单分子电子学进行 DNA 测序

• 批准号: 9172062
• 负责人: Boyan Boyanov
• 金额: $ 58.46万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-16 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9172062
• 关键词: Affect; Base Pairing; Binding; Biochemical; Carbon; Charge; Collaborations; DNA; DNA Sequence; DNA-Directed DNA Polymerase; Data; Development; Devices; Discrimination; Disease; Electronics; Enzyme Kinetics; Enzymes; Event; Foundations; Generations; Goals; Health Care Research; Healthcare; Heart; Hybrids; Individual; Laboratory Research; Lead; Machine Learning; Marketing; Massive Parallel Sequencing; Methods; Modality; Modification; Molecular Models; Monitor; Motion; Mutation; Nanotechnology; Noise; Nucleotides; Optics; Performance; Polymerase; Process; Protein Engineering; Proteins; Publishing; Reading; Research; Research Project Grants; Resolution; Route; Scientist; Signal Transduction; Single-Stranded DNA; Site; Staging; Surface; System; Techniques; Technology; Temperature; Transistors; Variant; Work; base; collaborative environment; cost; enzyme activity; improved; molecular modeling; nanoelectronics; nanopore; nanoscale; new technology; novel; research study; response; scale up; single molecule; single walled carbon nanotube; solid state

PROJECT SUMMARY / ABSTRACT Progress in DNA sequencing has occurred through multiple stages of disruptive new technologies being introduced to the field, each of which has increased sequencing capabilities by lowering costs, improving throughput, and reducing errors. The goal of this research project is to investigate a new, all-electronic sequencing method that has the potential to become the next transformative step for DNA sequencing. This new method is based on single DNA polymerase molecules bound to nanoscale electronic transistors, a hybrid device that transduces the activity of a single polymerase molecule into an electronic signal. The goal of this research project is to determine whether these hybrid polymerase-transistors are truly applicable to DNA sequencing and the competitive environment of advanced sequencing technologies. To answer this question, the project teams the scientists who have developed the devices with Illumina, Inc., a worldwide leader in the DNA sequencing market. The experiments proposed here build on encouraging preliminary results, first to demonstrate accurate DNA sequencing and second to evaluate whether the new technique could become a competitive challenge to other sequencing methods. The interdisciplinary team will combine state-of-the-art techniques from protein engineering, nanoscale fabrication, and machine learning to customize polymerase's activity and its interactions with the electronic transistors. If successful, nanoscale solid-state devices like transistors provide one of the best opportunities for increasing sequencing capabilities while decreasing sequencing costs, so that DNA sequencing can become a standard technique in health care and disease treatment.

项目摘要/摘要 DNA测序的进步是通过多个阶段的颠覆性新技术实现的 引入现场，每一种都通过降低成本、改进 吞吐量，并减少错误。这项研究项目的目标是研究一种新型的、全电子的 有可能成为DNA测序的下一个变革性步骤的测序方法。这 新的方法是基于单个DNA聚合酶分子绑定到纳米级电子晶体管上，这是一种混合 将单个聚合酶分子的活性转化为电子信号的装置。 这项研究项目的目标是确定这些混合聚合酶晶体管是否真的 适用于DNA测序和先进测序技术的竞争环境。至 回答这个问题，该项目将与Illumina，Inc.合作开发设备的科学家组成团队 DNA测序市场的全球领导者。这里提出的实验建立在鼓舞人心的基础上 初步结果，首先要证明DNA测序准确，其次要评估新的 技术可能会成为对其他测序方法的竞争挑战。跨学科团队将 将蛋白质工程、纳米制造和机器学习等最先进的技术结合起来， 定制聚合酶的活性及其与电子晶体管的相互作用。如果成功，纳米级 像晶体管这样的固态设备为提高排序能力提供了最好的机会之一 同时降低测序成本，使DNA测序成为医疗卫生领域的标准技术 和疾病治疗。