Hybrid Nanopore Platform for DNA Sequencing

用于 DNA 测序的混合纳米孔平台

• 批准号: 8752109
• 负责人: Boyan Boyanov
• 金额: $ 29.57万
• 依托单位: ILLUMINA, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8752109
• 关键词: Address; Benchmarking; Biologic Characteristic; Biological; Caliber; Complex; DNA; DNA Sequence; Data; Engineering; Ensure; Goals; Hybrids; Length; Lipid Bilayers; Lipids; Methods; Morphology; Motivation; Pattern; Process; Proteins; Protocols documentation; Reading; Research; Route; Semiconductors; Shapes; Short Tandem Repeat; Speed; Structure; Surface; System; Tandem Repeat Sequences; Technology; Work; commercialization; cost; improved; nanodisk; nanofabrication; nanopore; porin; public health relevance; reconstitution; response; seal; silicon nitride; solid state; synthetic construct

DESCRIPTION (provided by applicant): The objective of the proposed research is to demonstrate hybrid nanopores combining top-down patterning and nanofabrication with bottom-up biological assembly. Existing nanopore sequencing, especially biological nanopores in lipid bilayers, achieve high speed and read length at the cost of decreased parallelization and data throughput. The fragility of the platform also makes it less practical in commercialization. The primary goal of this project is to establish a hybrid biological-solid state structure that can be self-assembled in a robust automated manner with high efficiency. Our preliminary research has identified plausible carriers of biological channels that can be assembled onto ~10nm solid-state nanopore. The proposed work will focus on demonstration of nanopore protein incorporation, self-limited channel insertion, and demonstration of biological nanopore activity with the hybrid platform. The proposed platform retains the speed and read length characteristics of biological nanopore systems while dramatically increasing the parallelization and system stability by leveraging established semiconductor technologies.

描述（由申请人提供）：拟议研究的目的是展示将​​自上而下的图案化和纳米加工与自下而上的生物组装相结合的混合纳米孔。现有的纳米孔测序，特别是脂质双层中的生物纳米孔，以降低并行化和数据吞吐量为代价实现了高速度和读长。该平台的脆弱性也使其在商业化方面不太实用。该项目的主要目标是建立一种混合生物固态结构，可以以稳健的自动化方式高效地进行自组装。我们的初步研究已经确定了可以组装到约 10 nm 固态纳米孔上的生物通道的合理载体。拟议的工作将集中于演示纳米孔蛋白质掺入、自限性通道插入以及利用混合平台演示生物纳米孔活性。所提出的平台保留了生物纳米孔系统的速度和读长特征，同时通过利用现有的半导体技术显着提高了并行化和系统稳定性。