Functions of G-proteins in Endothelial Cells

内皮细胞中 G 蛋白的功能

• 批准号: 9176372
• 负责人: Xin-Yun Huang
• 金额: $ 42.38万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9176372
• 关键词: Actins; Adult; Binding; Biochemical; Biological Assay; Biological Process; Blood Vessels; C-terminal; Capillary Endothelial Cell; Cardiovascular Diseases; Cause of Death; Cell Proliferation; Cell membrane; Cell physiology; Cells; Cessation of life; Crystallography; Cytoskeletal Modeling; DH Domain; Defect; Development; Embryo; Endothelial Cells; Fibroblasts; G-Protein-Coupled Receptors; GTP-Binding Proteins; Genetic study; Growth; Guanine Nucleotide Exchange Factors; Guanine Nucleotides; Health; Human; In Vitro; Knockout Mice; Lipids; Malignant Neoplasms; Membrane; Methods; Modification; Molecular; Mus; Nucleotides; Ovarian; Phosphotransferases; Physiologic Neovascularization; Physiological; Play; Proteins; Recruitment Activity; Regulation; Research; Retinal; Roentgen Rays; Role; Signal Pathway; Signal Transduction; Son; Stem cells; Study Section; Testing; Tube; Tumor Angiogenesis; Vascularization; Yolk Sac; angiogenesis; base; blood vessel development; cell type; combat; genetic regulatory protein; human disease; migration; novel; organ regeneration; postnatal; public health relevance

Project Description The main focus of this application is on the functions of G-proteins in endothelial cells. G-protein Gα13 is critical for blood vessel formation. This proposal builds on our discovery of a novel mechanism by which Gα13 functions through SOS, a direct regulator of Ras and the new observation that endothelial cell-specific deletion of Gα13 impairs organ regeneration. The first aim is to dissect the biochemical mechanism by which Gα13 regulates SOS in vitro. The second aim is to determine how Gα13 and G-protein- coupled receptors regulate SOS in cells. The third aim is to investigate the impact of endothelial cell-specific deletion of SOS1 on organ regeneration.

项目说明 这一应用的主要焦点是内皮细胞中G蛋白的功能。 G-蛋白G-α13对血管形成至关重要。这项建议建立在我们的 发现G-α13通过直接调节因子SOS发挥作用的新机制 RAS和内皮细胞特异性缺失Gα13损伤的新观察 器官再生。第一个目标是剖析生物化学机制，通过 G-α13在体外对SOS有调节作用。第二个目标是确定G-α13和G-蛋白是如何- 偶联受体调节细胞内的SOS。第三个目标是调查 血管内皮细胞特异性缺失SOS1对器官再生的影响。