Functions of G-proteins in Endothelial Cells

内皮细胞中 G 蛋白的功能

• 批准号: 9282797
• 负责人: Xin-Yun Huang
• 金额: $ 42.38万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-06-01 至 2020-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9282797
• 关键词: Actins; Adult; Binding; Biochemical; Biological Assay; Biological Process; Biophysics; Blood Vessels; C-terminal; Capillary Endothelial Cell; Cardiovascular Diseases; Cause of Death; Cell Proliferation; Cell membrane; Cell physiology; Cells; Cessation of life; Crystallography; Cytoskeletal Modeling; DH Domain; Defect; Development; Embryo; Endothelial Cells; Fibroblasts; G-Protein-Coupled Receptors; G-substrate; GTP-Binding Proteins; Genetic study; Growth Factor; Guanine Nucleotides; Health; Human; Impairment; In Vitro; Knockout Mice; Lipids; Malignant Neoplasms; Membrane; Methods; Modification; Molecular; Mus; Nucleotides; Ovarian; Pathologic; Phosphotransferases; Physiological; Play; Proteins; Recruitment Activity; Regulation; Research; Retinal; Roentgen Rays; Role; Signal Pathway; Signal Transduction; Son; Son of Sevenless Proteins; Stem cells; Study Section; Testing; Tube; Tumor Angiogenesis; Vascularization; Yolk Sac; angiogenesis; base; blood vessel development; cell type; combat; genetic regulatory protein; human disease; migration; novel; organ regeneration; postnatal; public health relevance; ras Guanine Nucleotide Exchange Factors

Project Description The main focus of this application is on the functions of G-proteins in endothelial cells. G-protein Gα13 is critical for blood vessel formation. This proposal builds on our discovery of a novel mechanism by which Gα13 functions through SOS, a direct regulator of Ras and the new observation that endothelial cell-specific deletion of Gα13 impairs organ regeneration. The first aim is to dissect the biochemical mechanism by which Gα13 regulates SOS in vitro. The second aim is to determine how Gα13 and G-protein- coupled receptors regulate SOS in cells. The third aim is to investigate the impact of endothelial cell-specific deletion of SOS1 on organ regeneration.

项目描述 本申请的主要焦点是G蛋白在内皮细胞中的功能。 G蛋白Gα13对血管形成至关重要。该提案建立在我们的 发现Gα13通过SOS（一种直接调节剂）发挥作用的新机制 和新的观察，即内皮细胞特异性Gα13缺失损害 器官再生第一个目的是剖析生物化学机制， Gα13在体外调节SOS。第二个目的是确定Gα13和G蛋白- 偶联受体调节细胞中的SOS。第三个目标是调查 内皮细胞特异性缺失SOS 1对器官再生的影响。