Flow Cytometry Cell Sorter
流式细胞仪细胞分选仪
基本信息
- 批准号:8825322
- 负责人:
- 金额:$ 44.29万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-04-01 至 2016-03-31
- 项目状态:已结题
- 来源:
- 关键词:AerosolsBiohazardous SubstanceCell SeparationCellsComputer softwareContract ServicesDisadvantagedEquus caballusFailureFamilyFlow CytometryFundingHousingInstitutionIowaLasersMaintenanceProviderRequest for ProposalsResearchResearch PersonnelRetirementSalesSamplingServicesSorting - Cell MovementSpeedSystemUnited States National Institutes of HealthUniversitiesWaterWorkcollegeinstrumentresearch study
项目摘要
DESCRIPTION (provided by applicant): The University of Iowa Flow Cytometry Facility, established in 1979, was used by 22 departments across four colleges in 2013. Serving this diverse group of investigators requires instruments capable of accommodating a wide range of demands including multi-parameter high-speed cell sorting in a biologically safe manner. The Facility currently operates two cell sorters, a Becton Dickinson (BD) FACS Vantage/DiVa and a FACS Aria II/SORP, both of which are used heavily. There is no question that two cell sorters are necessary to fulfill the obligations of the NIH-funded investigators at this institution. Priorto acquiring the second cell sorter (the FACS Aria II) in 2010, the Facility had a minimum of a one month backlog for sorting experiments. The investigators made it clear that this long waiting period was interfering with their ability to complete their projects and put them at a competitive disadvantage. It is quite clear that this Facility needs two sorters for optimal service delivery. This proposal requests funds for a biologically contained BD FACS Aria SORP cell sorter to replace the BD FACS Vantage/DiVa. While the BD FACS Vantage/DiVa, installed in January 2002, has served as a work horse for a broad range of cell sorting experiments, it has become more difficult to support. BD discontinued sales of the FACS Vantage/DiVa family of instruments in 2007, ended DiVa software upgrades in 2008, and stopped offering service contracts after 2010. The three water-cooled main-frame lasers also require significant maintenance and expense to support. Third party suppliers do not offer Vantage/DiVa parts other than preventative maintenance kits. With parts and support difficult to obtain, there is increased likelihood that an unrecoverable failure that will render the instrument unusable. Moreover, while the Facility's newer BD FACS Aria II/SORP has an aerosol management system and is housed in a biosafety cabinet, the BD FACS Vantage/DiVa only utilizes an aerosol management system for biohazard protection. This has limited the types of samples that can be sorted and thus the projects that can be accommodated with the FACS DiVa. The University of Iowa Flow Cytometry Facility is the sole cell-sorting provider on campus. The only other publicly accessible cell sorter in the state of Iowa is housed at Iowa State University, approximately 130 miles away. The inevitable retirement of the BD FACS Vantage/DiVa will result in a significant adverse impact on the timely completion of the research of numerous investigators if not replaced with a new cell sorter.
描述(由申请人提供):爱荷华州大学流式细胞仪设施,成立于1979年,2013年被四所学院的22个部门使用。服务于这一多样化的研究者群体需要能够满足广泛需求的仪器,包括以生物安全的方式进行多参数高速细胞分选。该设施目前运行两台细胞分选机,一台Becton Dickinson(BD)FACS Vantage/DiVa和一台FACS Aria II/SORP,这两台分选机都被大量使用。毫无疑问,两个细胞分选仪是必要的,以履行NIH资助的研究人员在该机构的义务。在2010年获得第二台细胞分选机(FACS Aria II)之前,该设施至少有一个月的分选实验积压。调查人员明确表示,这一漫长的等待期妨碍了他们完成项目的能力,使他们处于竞争劣势。很明显,该设施需要两台分拣机来提供最佳服务。该提案要求为生物包含的BD FACS Aria SORP细胞分选仪提供资金,以取代BD FACS Vantage/DiVa。虽然2002年1月安装的BD FACS Vantage/DiVa已经作为广泛的细胞分选实验的工作马,但它变得更加难以支持。BD于2007年停止销售FACS Vantage/DiVa系列仪器,于2008年结束DiVa软件升级,并于2010年后停止提供服务合同。三台水冷主机激光器也需要大量的维护和费用支持。第三方供应商不提供预防性维护套件以外的Vantage/DiVa零件。由于难以获得部件和支持,因此发生不可恢复故障的可能性增加,导致手术工具无法使用。此外,虽然该设施较新的BD FACS Aria II/SORP具有气溶胶管理系统,并安装在生物安全柜中,但BD FACS Vantage/DiVa仅使用气溶胶管理系统进行生物危害防护。这就限制了可以分类的样本类型,从而限制了流式细胞仪DiVa可以容纳的项目。爱荷华州大学流式细胞仪是校园内唯一的细胞分选提供商。爱荷华州唯一一个可公开使用的细胞分类器位于大约130英里外的爱荷华州州立大学。如果不更换新的细胞分选仪,BD FACS Vantage/DiVa不可避免的退役将对许多研究者及时完成研究产生重大不利影响。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
ZUHAIR K. BALLAS其他文献
ZUHAIR K. BALLAS的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}