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Flow Cytometry Cell Sorter

流式细胞仪细胞分选仪

基本信息

批准号：
7790807
负责人：
ZUHAIR K. BALLAS
金额：
$ 49.99万
依托单位：
UNIVERSITY OF IOWA
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-09-01 至 2010-08-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): The University of Iowa Flow Cytometry Facility, established in 1979, supports a broad base of campus research projects. The Facility has grown to include nine instruments used by 23 departments across five colleges. Serving a diverse group of investigators requires instruments capable of accommodating a wide range of experiments. High-speed, multi-color cell sorting is a major component of this service. The Facility has only housed three cell sorters over its 30- year history, each used consecutively. The instruments were replaced only when they became obsolete in terms of scientific merit or difficult to maintain because of lack of spare parts and/or manufacturer support. This indicates that public funds have been used responsibly and wisely in support of healthcare-related research at the University of Iowa. The Facility's current cell sorter, scheduled to be obsolete after 2010, is a 2002 Becton Dickinson FACS DiVa. It serves as a work horse for cloning, isolating cells expressing fluorescent protein markers, multi-color cell sorting of rare cell populations, sorting based on cell cycle, and stem cell sorting of side populations. This Shared Instrumentation Application requests funds for a Becton Dickinson FACS Aria high-speed, multi-color cell sorter to replace the current instrument and to increase the number of colors, sensitivity, and capacity it provides to investigators. The Facility's long-term goal is to remain as close to state of the art as possible in support of its investigators' research. A replacement cell sorter is absolutely necessary given the wide spectrum of institutions and investigators that will be impacted if there is no access to cell sorting. Becton Dickinson discontinued sales of the FACS Vantage SE/DiVa family of instruments in 2007, DiVa software upgrades in 2008, and service contracts after 2010 (see attached letter). When parts and software to support the instrument become difficult to obtain after 2010, the current instrument will be mothballed. Secondly, the other two publicly accessible cell sorters that were previously available in the state of Iowa were retired in 2008. One of those instruments was housed at Iowa State University, and the other was housed in the University Of Iowa Department Of Pathology. The Flow Cytometry Facility now has the only operational cell sorter at an Iowa Board of Regents institution and must provide service to a larger number of investigators. If this sorter is not replaced, investigators will not be able to execute their funded research. PUBLIC HEALTH RELEVANCE: University of Iowa researchers use flow cytometry, a fluorescence-based tool, to study human diseases such as prostate and breast cancer, lymphoma, cancer metastasis, autoimmune disease, neurodegenerative disease, influenza, SARS infection, cellular effects of PCB pollutants, and Stem cell therapies. The cells that may hold the key to understanding and treating these diseases are usually present in minuscule numbers and cannot be readily examined. Sorting by flow cytometry allows the investigators to obtain large amounts of these important cells which can then be examined.

描述(由申请者提供)：爱荷华大学流式细胞术设施成立于1979年，支持广泛的校园研究项目。该设施已发展到包括五所学院的23个系使用的9种仪器。为不同的研究人员群体服务需要能够容纳广泛实验的仪器。高速、多色细胞分选是这项服务的主要组成部分。该设施在其30年的历史中只容纳了三个细胞分类器，每个都连续使用。这些仪器只有在其科学价值变得过时或由于缺乏备件和/或制造商支持而难以维护时才被更换。这表明，在支持爱荷华大学与医疗保健相关的研究方面，公共资金得到了负责任和明智的使用。该设施目前的细胞分类器，计划在2010年后淘汰，是2002年的Becton Dickinson FACS DIVA。它是克隆、分离表达荧光蛋白标记物的细胞、稀有细胞群的多色细胞分选、基于细胞周期的分选以及副细胞群的干细胞分选的工作马。该共享仪器应用程序要求为Becton Dickinson FACS Aria高速多色细胞分选机提供资金，以取代当前的仪器，并增加为调查人员提供的颜色数量、灵敏度和容量。该设施的长期目标是尽可能接近最先进的技术，以支持其研究人员的研究。考虑到如果无法获得细胞分类将受到影响的机构和调查人员的广泛范围，更换细胞分选机是绝对必要的。Becton Dickinson在2007年停止了FACS Vantage SE/DIVA系列仪器的销售，在2008年停止了DIVA软件升级，并在2010年后停止了服务合同(见附信)。2010年后，当支持该仪器的部件和软件变得难以获得时，当前的仪器将被封存。其次，爱荷华州以前提供的另外两台可公开使用的细胞分类器已于2008年退役。其中一台仪器安装在爱荷华州立大学，另一台安装在爱荷华大学病理学系。流式细胞术设施现在拥有爱荷华州董事会机构中唯一可运行的细胞分选器，必须为更多的调查人员提供服务。如果不更换这个分类器，研究人员将无法执行他们资助的研究。公共卫生相关性：爱荷华大学的研究人员使用基于荧光的工具流式细胞术来研究人类疾病，如前列腺癌和乳腺癌、淋巴瘤、癌症转移、自身免疫性疾病、神经退行性疾病、流感、SARS感染、多氯联苯污染物的细胞效应和干细胞疗法。可能掌握着理解和治疗这些疾病的关键的细胞通常数量很少，不容易检查。通过流式细胞术进行分类，研究人员可以获得大量这些重要的细胞，然后进行检查。