Full Spectrum Cell Sorter Flow Cytometer

全光谱细胞分选仪流式细胞仪

• 批准号: 10625118
• 负责人: ZUHAIR K. BALLAS
• 金额: $ 52.58万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-15 至 2024-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10625118
• 关键词: Administrator; Biohazardous Substance; Biomedical Research; Cell Separation; Cells; Color; Computer software; Contract Services; Disadvantaged; Disease; Ensure; Equus caballus; Experimental Designs; Failure; Family; Flow Cytometry; Funding; Goals; Health; Human; Institution; Iowa; Maintenance; Principal Investigator; Provider; Request for Proposals; Research; Research Personnel; Research Project Grants; Retirement; Safety; Sales; Sampling; Sorting; Speed; Time; United States National Institutes of Health; Universities; Update; Work; college; experimental study; improved; instrument; instrumentation; working group

PROJECT SUMMARY/ABSTRACT The University of Iowa Flow Cytometry Facility was established in 1979 and served 140 principal investigators spanning 33 departments across four colleges in 2021. This group works on diverse biomedical research projects and requires instruments capable of accommodating a wide range of demands, including high- parameter high-speed cell sorting in a biologically safe manner. Our Facility currently operates two heavily used cell sorters, a Becton Dickinson (BD) FACSAria Fusion SORP and a BD FACS Aria II/SORP. Prior to acquiring a second cell sorter in 2010, the Facility had a minimum of a one-month backlog for sorting experiments, which interfered with the ability of investigators to complete projects efficiently, putting them at a competitive disadvantage. Given the number of users and complexity of the research being conducted at our facility, two cell sorters are necessary to meet research demands of NIH-funded investigators at this institution. The Facility’s FACS Aria II/SORP was installed in May 2010 and serves as a work horse for a broad range of cell sorting experiments. However, over time it has become more difficult to support. BD discontinued sales of the FACS Aria II/SORP family of instruments in 2017 and stopped offering service contracts in early 2021. Instrument software cannot be updated without removing the administrator profile, which effectively ends software support. Third party suppliers do not offer FACS Aria II/SORP parts. BD no longer sells customer preventative maintenance kits. With parts and support difficult to obtain, it is increasingly likely that an unrecoverable failure will render the instrument unusable. Notably, our facility is the sole cell-sorting provider on campus. The only other publicly accessible cell sorter in the state of Iowa is housed at Iowa State University, approximately 130 miles away. The inevitable retirement or failure of the FACS Aria II/SORP will have a significant adverse impact on the timely completion of the research projects of numerous investigators. This proposal requests funds for a Cytek Aurora CS cell sorter to replace our BD FACS Aria II/SORP. We are also requesting funds to operate this cell sorter in a biohazard safety cabinet, as the Facility’s current cell sorters are housed in biosafety cabinets, and thus investigators have incorporated the ability to sort biohazardous samples into their experimental designs. The addition of the Cytek Aurora CS will increase the number of colors, sensitivity, and capacity provided to investigators for cell sorting experiments and will allow for continued support and enhancement of research projects carried out at the University of Iowa and in the State of Iowa. This will ensure the Facility continues to meet its overall goal of providing state-of-the-art instrumentation that supports biomedical research to improve our understanding of human health and disease.

项目摘要/摘要 爱荷华大学流式细胞术设施成立于1979年，为140名主要研究人员提供服务 2021年将覆盖四所大学的33个系。这个小组从事不同的生物医学研究 项目，并需要能够满足广泛需求的工具，包括高 参数以生物安全的方式进行高速细胞分选。我们的设施目前运行着两个重型 使用细胞分选机，Becton Dickinson(BD)FACSAria Fusion SORP和BD FACS Aria II/SORP。在.之前 在2010年购买了第二台细胞分类器后，该设施至少有一个月的积压时间来进行分类 实验干扰了研究人员高效完成项目的能力，使他们处于一种 竞争劣势。考虑到用户的数量和正在进行的研究的复杂性 为了满足NIH资助的研究人员在该机构的研究需求，两台细胞分选机是必要的。 该设施的FACS Aria II/SORP于2010年5月安装，是广泛的 细胞分选实验。然而，随着时间的推移，支持它变得更加困难。屋宇署停止销售 FACS Aria II/SORP系列仪器于2017年推出，并于2021年初停止提供服务合同。 在不删除管理员配置文件的情况下无法更新仪器软件，这实际上会结束 软件支持。第三方供应商不提供FACS Aria II/SORP部件。BD不再销售客户 预防性维护套件。由于很难获得部件和支持，越来越有可能出现 不可恢复的故障将使仪器无法使用。值得注意的是，我们的设施是唯一的细胞分类提供商 在校园里。爱荷华州唯一另一个可公开访问的细胞分类器位于爱荷华州 大学，大约130英里外。FACS Aria II/SORP将不可避免地退役或失败 对众多调查人员及时完成研究项目造成重大不利影响。 这项提案要求为Cytek Aurora CS细胞分选机提供资金，以取代我们的BD FACS Aria II/SORP。我们是 还申请资金在生物危害安全柜中操作这个细胞分类器，作为该设施的当前细胞 分类器被安置在生物安全柜中，因此调查人员已经纳入了分类的能力 将对生物有害的样本纳入他们的实验设计。Cytek Aurora CS的加入将增加 为调查人员提供用于细胞分选实验的颜色、灵敏度和容量的数量，并将允许 继续支持和加强在爱荷华大学和 爱荷华州。这将确保该基金继续实现其总体目标，即提供最先进的 支持生物医学研究的仪器，以提高我们对人类健康和疾病的理解。