Full Spectrum Cell Sorter Flow Cytometer

全光谱细胞分选仪流式细胞仪

• 批准号: 10625118
• 负责人: ZUHAIR K. BALLAS
• 金额: $ 52.58万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-15 至 2024-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10625118
• 关键词: Administrator; Biohazardous Substance; Biomedical Research; Cell Separation; Cells; Color; Computer software; Contract Services; Disadvantaged; Disease; Ensure; Equus caballus; Experimental Designs; Failure; Family; Flow Cytometry; Funding; Goals; Health; Human; Institution; Iowa; Maintenance; Principal Investigator; Provider; Request for Proposals; Research; Research Personnel; Research Project Grants; Retirement; Safety; Sales; Sampling; Sorting; Speed; Time; United States National Institutes of Health; Universities; Update; Work; college; experimental study; improved; instrument; instrumentation; working group

PROJECT SUMMARY/ABSTRACT The University of Iowa Flow Cytometry Facility was established in 1979 and served 140 principal investigators spanning 33 departments across four colleges in 2021. This group works on diverse biomedical research projects and requires instruments capable of accommodating a wide range of demands, including high- parameter high-speed cell sorting in a biologically safe manner. Our Facility currently operates two heavily used cell sorters, a Becton Dickinson (BD) FACSAria Fusion SORP and a BD FACS Aria II/SORP. Prior to acquiring a second cell sorter in 2010, the Facility had a minimum of a one-month backlog for sorting experiments, which interfered with the ability of investigators to complete projects efficiently, putting them at a competitive disadvantage. Given the number of users and complexity of the research being conducted at our facility, two cell sorters are necessary to meet research demands of NIH-funded investigators at this institution. The Facility’s FACS Aria II/SORP was installed in May 2010 and serves as a work horse for a broad range of cell sorting experiments. However, over time it has become more difficult to support. BD discontinued sales of the FACS Aria II/SORP family of instruments in 2017 and stopped offering service contracts in early 2021. Instrument software cannot be updated without removing the administrator profile, which effectively ends software support. Third party suppliers do not offer FACS Aria II/SORP parts. BD no longer sells customer preventative maintenance kits. With parts and support difficult to obtain, it is increasingly likely that an unrecoverable failure will render the instrument unusable. Notably, our facility is the sole cell-sorting provider on campus. The only other publicly accessible cell sorter in the state of Iowa is housed at Iowa State University, approximately 130 miles away. The inevitable retirement or failure of the FACS Aria II/SORP will have a significant adverse impact on the timely completion of the research projects of numerous investigators. This proposal requests funds for a Cytek Aurora CS cell sorter to replace our BD FACS Aria II/SORP. We are also requesting funds to operate this cell sorter in a biohazard safety cabinet, as the Facility’s current cell sorters are housed in biosafety cabinets, and thus investigators have incorporated the ability to sort biohazardous samples into their experimental designs. The addition of the Cytek Aurora CS will increase the number of colors, sensitivity, and capacity provided to investigators for cell sorting experiments and will allow for continued support and enhancement of research projects carried out at the University of Iowa and in the State of Iowa. This will ensure the Facility continues to meet its overall goal of providing state-of-the-art instrumentation that supports biomedical research to improve our understanding of human health and disease.

项目总结/摘要 爱荷华州大学的流式细胞术设施成立于1979年，为140名主要研究者服务 在2021年跨越四个学院的33个部门。该小组致力于多样化的生物医学研究 项目，并要求文书能够满足广泛的需求，包括高， 以生物安全的方式进行参数高速细胞分选。我们的设施目前经营两个沉重的 使用细胞分选仪，Becton Dickinson（BD）FACSAria Fusion SORP和BD FACS Aria II/SORP。之前 在2010年购买了第二台细胞分选机后，该设施至少有一个月的分选积压 实验，这干扰了调查人员有效完成项目的能力，使他们处于 竞争劣势。考虑到用户的数量和我们正在进行的研究的复杂性， 设施，两个细胞分选机是必要的，以满足NIH资助的研究人员在该机构的研究需求。 2010年5月安装了该设施的流式细胞仪Aria II/SORP， 细胞分选实验。然而，随着时间的推移，它变得越来越难以支持。BD停止销售 该公司于2017年收购了FACS Aria II/SORP系列仪器，并于2021年初停止提供服务合同。 如果不删除管理员配置文件，则无法更新仪器软件， 软件支持。第三方供应商不提供FACS Aria II/SORP部件。BD不再销售客户 预防性维护套件。由于难以获得零件和支持， 不可恢复的故障将使仪器无法使用。值得注意的是，我们的设施是唯一的细胞分选供应商 在校园里在爱荷华州，唯一的另一个可公开访问的细胞分类器位于爱荷华州 大学，大约130英里远。不可避免的退役或失败的流式细胞仪阿里亚二/SORP将 对许多调查人员及时完成研究项目产生重大不利影响。 该提案要求为Cytek Aurora CS细胞分选机提供资金，以取代我们的BD FACS Aria II/SORP。我们 还要求资金在生物危害安全柜中操作该细胞分选机，作为该设施的当前细胞 分类器被安置在生物安全柜中，因此研究人员已经将分类能力 生物危害性样本加入到他们的实验设计中Cytek Aurora CS的加入将增加 提供给研究人员进行细胞分选实验的颜色数量、灵敏度和容量， 继续支持和加强在爱荷华州大学和 爱荷华州。这将确保该基金继续实现其提供最先进的 支持生物医学研究的仪器，以提高我们对人类健康和疾病的理解。