IGF::OT::IGF R&D BIOMEDICAL RESEARCH

液体活检的可用性，如血液取样来测量关键的疾病生物标志物，可能有助于检测已知的赋予癌症治疗耐药性的血液循环肿瘤DNA （ctDNA）。例如，表皮生长因子（EGFR）的T790M突变占fda批准的EGFR靶向治疗获得性耐药的50%。检测这种突变对于评估疾病复发和指导选择替代疗法以获得更好的治疗效果非常重要。因此，鉴定含有egfr特异性耐药突变的ctdna需要是非侵入性的。然而，检测ctdna具有挑战性，因为它们只占血液中正常循环DNA总数的一小部分。目前的检测方法依赖于基于数字pcr的技术，由于成本高、需要训练有素的操作人员、需要耗时的样品纯化步骤、样品到答案的时间慢以及对大量生物标记物进行并行分析的能力有限，这些技术受到限制。为了满足这一需求，Giner公司提出开发一种电化学生物传感器，用于多重检测与egfr靶向治疗获得性耐药相关的ctDNA生物标志物。该传感器将被设计用于实现人类血清中ctDNA的选择性和敏感性检测

The availability of liquid biopsies, such as sampling of blood to measure key disease biomarkers, could aid in the detection of blood-circulating tumor DNA (ctDNA) known to confer resistance to cancer therapy. For example, the T790M mutation in the epidermal growth factor (EGFR) accounts for 50% of acquired resistance to FDA-approved EGFR targeted therapies. Detection of this mutation is important to evaluate disease relapse and guide the selection of alternative therapies for better treatment outcome. Therefore, identification of ctDNAs that harbor EGFR-specific drug resistant mutations needs to be noninvasive. However, detection of ctDNAs is challenging because they constitute a small fraction of the total number of normal circulating DNA in blood. Current detection methods rely on digital PCR-based techniques, which are limited because of high cost, need for highly trained operators, requirement for time-consuming sample purification steps, slow sample-to-answer time, and limited ability for parallel analysis of a large number of biomarkers. To address this need, Giner, Inc. proposes to develop an electrochemical biosensor for multiplexed detection of ctDNA biomarkers associated with acquired resistance to EGFR-targeted therapies. This sensor will be designed to achieve both selective and sensitive detection of ctDNA in human serum at physiological relevant concentrations.