Sony Cell Sorter SH800 System

索尼细胞分选仪 SH800 系统

• 批准号: 9075724
• 负责人: David W Pascual
• 金额: $ 29.56万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075724
• 关键词: Aerosols; Animals; Anthrax disease; Applications Grants; Aryl Hydrocarbon Receptor; Autoimmune Diseases; Biological Containment; Botulism; Brucellosis; Cell Separation; Cells; Colitis; Collaborations; Communicable Diseases; Dentistry; Development; Effectiveness; Epithelial Cells; Equipment; Florida; Flow Cytometry; Funding; Goals; Health; Housing; Human; Immunologist; Intestines; Investigation; Leukocytes; Lymphocyte; Lymphoid Cell; Medicine; Minor; Mouth Neoplasms; Norovirus; Oral mucous membrane structure; Periodontal Diseases; Plague; Process; Program Development; Regulatory T-Lymphocyte; Research; Research Personnel; Safety; Sampling; Signal Transduction; Sjogren' s Syndrome; Speed; System; Tissue Sample; Tuberculosis; United States National Institutes of Health; Universities; Veterinary Medicine; Work; animal tissue; college; cost; cost effective; design; enteric pathogen; gut microbiota; human tissue; instrument; meetings; mucosal vaccine; response; user-friendly

 DESCRIPTION (provided by applicant): The long-term goal of the College of Veterinary Medicine (CVM) Flow Cytometry Core is to have a flow cytometry facility complete with analytical instruments and one instrument for cell-sorting. This equipment grant proposal seeks to expand its capacity for cell-sorting to obtain lymphocyte and leukocyte subsets to >95% cell purity. The Sony SH800 Cell Sorter, proposed herein, will fulfill this shortcoming of having a user-friendly system for high- speed cell-sorting for use by a diverse, but highly interactive grou of mucosal immunologists with a strong focus in one-health investigations. With the recent expansion of mucosal immunologists in CVM, the College of Medicine (CoM), and the College of Dentistry (CoD) at the University of Florida (UF), this group desires a cost- effective instrument not requiring a dedicated operator, and an instrument that can be supported by this core. Given that the greatest concentration of mucosal immunologists has been in CVM, the proposed Sony SH800 will be housed in CVM, but readily accessible to all major users within the three colleges as well as to other investigators within CVM having a need for cell-sorting for their health-related studies. With the availability of the Sony SH800, we envision it will immediately support, at the minimum, of 8 major users and one minor user. Cell- sorting will be used to fulfill study objectives in currently NIH-funded programs for development of mucosal vaccines for brucellosis, anthrax, botulism, enteric pathogens, plague, tuberculosis, and norovirus. In addition, this instrument will facilitate studies understanding the basic mechanisms of aryl hydrocarbon receptor signaling for the development of mucosal Th17 cells and regulatory T cells in colitis; studies understanding the TCR usage in animals and humans with Sjögren's Syndrome; studies understanding the influences of the gut microbiota upon intestinal epithelial and lymphoid cells in the normal and inflamed gut; and studies understanding how TLR signaling via the oral mucosa impacts lymphocyte responses to periodontal disease and oral neoplasms. Included in this proposal is the Baker Biological Containment Cabinet specifically designed to house the Sony SH800 to contain aerosols produced during cell-sorting. Since this collective group of investigators work with infected animal and human tissue samples, this barrier provides a needed additional level of safety for both the operators and samples processed. In summary, having the capacity for high-speed cell-sorting at a reasonable cost will further the current collaborations amongst these mucosal immunologists, help them meet their study objectives for ongoing NIH-funded research, and expand their ongoing effectiveness in infectious and autoimmune disease research.

 描述（由申请人提供）：兽医学院（CVM）流式细胞术核心的长期目标是拥有一个配有分析仪器和一台细胞分选仪器的流式细胞术设施。该设备拨款提案旨在扩大其细胞分选能力，以获得淋巴细胞和白细胞亚群，使细胞纯度达到 >95%。本文提出的索尼 SH800 细胞分选仪将弥补这一缺点，即拥有一个用户友好的系统，用于高速细胞分选，供不同但高度互动的粘膜免疫学家群体使用，重点关注单一健康研究。最近，随着佛罗里达大学 (UF) CVM、医学院 (CoM) 和牙科学院 (CoD) 粘膜免疫学家的规模不断扩大，该小组需要一种经济高效的仪器，不需要专门的操作员，并且可以由该核心支持的仪器。鉴于粘膜免疫学家最集中的地方是 CVM，拟议的索尼 SH800 将安装在 CVM 中，但三所学院内的所有主要用户以及 CVM 内需要进行细胞分选以进行健康相关研究的其他研究人员都可以轻松使用。随着索尼 SH800 的推出，我们预计它将立即支持至少 8 个主要用户和 1 个次要用户。细胞分选将用于实现当前 NIH 资助的针对布鲁氏菌病、炭疽病、肉毒杆菌病、肠道病原体、鼠疫、结核病和诺如病毒的粘膜疫苗开发计划的研究目标。此外，该仪器将有助于研究了解芳烃受体信号传导在结肠炎中粘膜 Th17 细胞和调节性 T 细胞发育的基本机制；研究了解 TCR 在患有干燥综合症的动物和人类中的应用；研究了解肠道微生物群对正常和发炎肠道中肠上皮细胞和淋巴细胞的影响；并研究了解通过口腔粘膜的 TLR 信号传导如何影响淋巴细胞对牙周病和口腔肿瘤的反应。该提案中包括贝克生物密封柜，专门用于容纳索尼 SH800，以容纳细胞分选过程中产生的气溶胶。由于这个研究人员集体研究受感染的动物和人体组织样本，因此这一屏障为操作员和处理的样本提供了所需的额外安全水平。总之，以合理的成本拥有高速细胞分选能力将进一步促进这些粘膜免疫学家之间的当前合作，帮助他们实现 NIH 资助的正在进行的研究的研究目标，并扩大他们在传染病和自身免疫性疾病研究中的持续有效性。