Sony Cell Sorter SH800 System

 描述（由申请人提供）：兽医学院（CVM）流式细胞术核心的长期目标是拥有一个流式细胞术设施，配备分析仪器和一台细胞分选仪器。该设备赠款提案旨在扩大其细胞分选能力，以获得淋巴细胞和白细胞亚群，使细胞纯度>95%。本文提出的Sony SH 800细胞分选仪将满足具有用于高速细胞分选的用户友好系统的缺点，以供不同但高度互动的格鲁粘膜免疫学家群体使用，这些粘膜免疫学家强烈关注单一健康研究。随着最近在佛罗里达大学（UF）的CVM、医学院（CoM）和牙科学院（CoD）中粘膜免疫学家的扩展，这一群体期望一种不需要专门操作者的成本有效的仪器，以及一种可以由该核心支持的仪器.鉴于粘膜免疫学家最集中的地方是CVM，拟议的Sony SH 800将被安置在CVM中，但三所学院内的所有主要用户以及CVM内需要进行细胞分选以进行健康相关研究的其他研究人员都可以随时使用。随着索尼SH 800的推出，我们预计它将立即支持至少8个主要用户和一个次要用户。细胞分选将用于实现目前NIH资助的用于开发布鲁氏菌病、炭疽、肉毒杆菌中毒、肠道病原体、鼠疫、结核病和诺如病毒的粘膜疫苗的项目中的研究目的。此外，这一工具将有助于开展研究，了解芳香烃受体信号传导对结肠炎中粘膜Th 17细胞和调节性T细胞发育的基本机制;了解患有舍格伦综合征的动物和人类中TCR使用情况的研究;了解肠道微生物群对正常和发炎肠道中肠上皮细胞和淋巴细胞影响的研究;并研究了解TLR信号如何通过口腔粘膜影响淋巴细胞对牙周病和口腔肿瘤的反应。本提案中包括专门设计用于容纳Sony SH 800的Baker生物密闭柜，以容纳细胞分选过程中产生的气溶胶。由于这一研究人员集体使用受感染的动物和人体组织样本，因此该屏障为操作员和处理的样本提供了所需的额外安全水平。总之，以合理的成本进行高速细胞分选的能力将进一步促进这些粘膜免疫学家之间的合作，帮助他们实现正在进行的NIH资助研究的研究目标，并扩大他们在传染病和自身免疫性疾病研究中的持续有效性。

 DESCRIPTION (provided by applicant): The long-term goal of the College of Veterinary Medicine (CVM) Flow Cytometry Core is to have a flow cytometry facility complete with analytical instruments and one instrument for cell-sorting. This equipment grant proposal seeks to expand its capacity for cell-sorting to obtain lymphocyte and leukocyte subsets to >95% cell purity. The Sony SH800 Cell Sorter, proposed herein, will fulfill this shortcoming of having a user-friendly system for high- speed cell-sorting for use by a diverse, but highly interactive grou of mucosal immunologists with a strong focus in one-health investigations. With the recent expansion of mucosal immunologists in CVM, the College of Medicine (CoM), and the College of Dentistry (CoD) at the University of Florida (UF), this group desires a cost- effective instrument not requiring a dedicated operator, and an instrument that can be supported by this core. Given that the greatest concentration of mucosal immunologists has been in CVM, the proposed Sony SH800 will be housed in CVM, but readily accessible to all major users within the three colleges as well as to other investigators within CVM having a need for cell-sorting for their health-related studies. With the availability of the Sony SH800, we envision it will immediately support, at the minimum, of 8 major users and one minor user. Cell- sorting will be used to fulfill study objectives in currently NIH-funded programs for development of mucosal vaccines for brucellosis, anthrax, botulism, enteric pathogens, plague, tuberculosis, and norovirus. In addition, this instrument will facilitate studies understanding the basic mechanisms of aryl hydrocarbon receptor signaling for the development of mucosal Th17 cells and regulatory T cells in colitis; studies understanding the TCR usage in animals and humans with Sjögren's Syndrome; studies understanding the influences of the gut microbiota upon intestinal epithelial and lymphoid cells in the normal and inflamed gut; and studies understanding how TLR signaling via the oral mucosa impacts lymphocyte responses to periodontal disease and oral neoplasms. Included in this proposal is the Baker Biological Containment Cabinet specifically designed to house the Sony SH800 to contain aerosols produced during cell-sorting. Since this collective group of investigators work with infected animal and human tissue samples, this barrier provides a needed additional level of safety for both the operators and samples processed. In summary, having the capacity for high-speed cell-sorting at a reasonable cost will further the current collaborations amongst these mucosal immunologists, help them meet their study objectives for ongoing NIH-funded research, and expand their ongoing effectiveness in infectious and autoimmune disease research.