Sony Cell Sorter SH800 System

索尼细胞分选仪 SH800 系统

基本信息

  • 批准号:
    9075724
  • 负责人:
  • 金额:
    $ 29.56万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-05-01 至 2017-04-30
  • 项目状态:
    已结题

项目摘要

 DESCRIPTION (provided by applicant): The long-term goal of the College of Veterinary Medicine (CVM) Flow Cytometry Core is to have a flow cytometry facility complete with analytical instruments and one instrument for cell-sorting. This equipment grant proposal seeks to expand its capacity for cell-sorting to obtain lymphocyte and leukocyte subsets to >95% cell purity. The Sony SH800 Cell Sorter, proposed herein, will fulfill this shortcoming of having a user-friendly system for high- speed cell-sorting for use by a diverse, but highly interactive grou of mucosal immunologists with a strong focus in one-health investigations. With the recent expansion of mucosal immunologists in CVM, the College of Medicine (CoM), and the College of Dentistry (CoD) at the University of Florida (UF), this group desires a cost- effective instrument not requiring a dedicated operator, and an instrument that can be supported by this core. Given that the greatest concentration of mucosal immunologists has been in CVM, the proposed Sony SH800 will be housed in CVM, but readily accessible to all major users within the three colleges as well as to other investigators within CVM having a need for cell-sorting for their health-related studies. With the availability of the Sony SH800, we envision it will immediately support, at the minimum, of 8 major users and one minor user. Cell- sorting will be used to fulfill study objectives in currently NIH-funded programs for development of mucosal vaccines for brucellosis, anthrax, botulism, enteric pathogens, plague, tuberculosis, and norovirus. In addition, this instrument will facilitate studies understanding the basic mechanisms of aryl hydrocarbon receptor signaling for the development of mucosal Th17 cells and regulatory T cells in colitis; studies understanding the TCR usage in animals and humans with Sjögren's Syndrome; studies understanding the influences of the gut microbiota upon intestinal epithelial and lymphoid cells in the normal and inflamed gut; and studies understanding how TLR signaling via the oral mucosa impacts lymphocyte responses to periodontal disease and oral neoplasms. Included in this proposal is the Baker Biological Containment Cabinet specifically designed to house the Sony SH800 to contain aerosols produced during cell-sorting. Since this collective group of investigators work with infected animal and human tissue samples, this barrier provides a needed additional level of safety for both the operators and samples processed. In summary, having the capacity for high-speed cell-sorting at a reasonable cost will further the current collaborations amongst these mucosal immunologists, help them meet their study objectives for ongoing NIH-funded research, and expand their ongoing effectiveness in infectious and autoimmune disease research.
 描述(由申请人提供):兽医学院(CVM)流式细胞术核心的长期目标是拥有一套完整的分析仪器和一台用于细胞分选的仪器。这项设备拨款提案旨在扩大其细胞分选能力,以获得细胞纯度达到95%的淋巴细胞和白细胞亚群。在此提出的Sony SH800细胞分选器将弥补这一缺点,即拥有一个用户友好的高速细胞分选系统,供各种高度互动的粘膜免疫学家使用,重点放在单一健康调查中。随着最近CVM、医学院(COM)和佛罗里达大学(UF)牙科学院(CoD)黏膜免疫学家的扩大,这一群体希望获得一种不需要专门操作员的经济高效的工具,以及一种可以由该核心支持的工具。鉴于黏膜免疫学家最集中在CVM,拟议的Sony SH800将安装在CVM中,但三所学院内的所有主要用户以及CVM内需要进行与健康相关研究的细胞分类的其他研究人员都可以随时使用。随着索尼SH800的上市,我们预计它将立即支持至少8个主要用户和1个次要用户。细胞分选将用于实现目前由NIH资助的开发布鲁氏菌病、炭疽病、肉毒杆菌中毒、肠道病原体、鼠疫、结核病和诺沃克病毒黏膜疫苗的研究目标。此外,该仪器还将有助于研究了解芳烃受体信号对结肠炎黏膜Th17细胞和调节性T细胞发育的基本机制;了解TCR在动物和患有干燥综合征的人类中的使用;帮助研究了解肠道微生物区系对正常和发炎肠道的肠道上皮细胞和淋巴样细胞的影响;以及了解TLR信号通过口腔粘膜如何影响淋巴细胞对牙周病和口腔肿瘤的反应。这项提案包括贝克生物安全柜,专门设计用于容纳索尼SH800,以容纳在细胞分选过程中产生的气雾剂。由于这一集体调查小组处理的是受感染的动物和人类组织样本,这一屏障为操作员和处理的样本提供了必要的额外安全水平。总而言之,拥有以合理成本进行高速细胞分选的能力将促进这些粘膜免疫学家之间目前的合作,帮助他们实现正在进行的NIH资助研究的研究目标,并扩大他们在传染病和自身免疫性疾病研究中的持续有效性。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

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David W Pascual其他文献

David W Pascual的其他文献

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{{ truncateString('David W Pascual', 18)}}的其他基金

Snodgrassella alvi as an attenuated live vaccine against Neisseria gonorrhoeae
Snodgrassella alvi 作为针对淋病奈瑟菌的减毒活疫苗
  • 批准号:
    10263891
  • 财政年份:
    2020
  • 资助金额:
    $ 29.56万
  • 项目类别:
Naso-oropharyngeal Brucella Infections and Mucosal Immune Protection
鼻口咽布鲁氏菌感染与粘膜免疫保护
  • 批准号:
    9751725
  • 财政年份:
    2017
  • 资助金额:
    $ 29.56万
  • 项目类别:
Regulatory Cell Therapy for Sjogrens Syndrome
干燥综合征的调节细胞疗法
  • 批准号:
    10898203
  • 财政年份:
    2017
  • 资助金额:
    $ 29.56万
  • 项目类别:
Naso-oropharyngeal Brucella Infections and Mucosal Immune Protection
鼻口咽布鲁氏菌感染与粘膜免疫保护
  • 批准号:
    10213597
  • 财政年份:
    2017
  • 资助金额:
    $ 29.56万
  • 项目类别:
Naso-oropharyngeal Brucella Infections and Mucosal Immune Protection
鼻口咽布鲁氏菌感染与粘膜免疫保护
  • 批准号:
    9977090
  • 财政年份:
    2017
  • 资助金额:
    $ 29.56万
  • 项目类别:
Mucosal Vaccines for Brucellosis
布鲁氏菌病粘膜疫苗
  • 批准号:
    9079710
  • 财政年份:
    2016
  • 资助金额:
    $ 29.56万
  • 项目类别:
Fimbriae Countermeasures for Type 1 Diabetes
1 型糖尿病的菌毛对策
  • 批准号:
    9242580
  • 财政年份:
    2016
  • 资助金额:
    $ 29.56万
  • 项目类别:
"Subunit Vaccines for Brucella Pathogens"
“布鲁氏菌病原体亚单位疫苗”
  • 批准号:
    8651868
  • 财政年份:
    2011
  • 资助金额:
    $ 29.56万
  • 项目类别:
"Subunit Vaccines for Brucella Pathogens"
“布鲁氏菌病原体亚单位疫苗”
  • 批准号:
    8827663
  • 财政年份:
    2011
  • 资助金额:
    $ 29.56万
  • 项目类别:
"Subunit Vaccines for Brucella Pathogens"
“布鲁氏菌病原体亚单位疫苗”
  • 批准号:
    8076096
  • 财政年份:
    2011
  • 资助金额:
    $ 29.56万
  • 项目类别:

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