Wide field of view 3D microscopy for calcium imaging and photostimulation

用于钙成像和光刺激的宽视场 3D 显微镜

基本信息

  • 批准号:
    9201872
  • 负责人:
  • 金额:
    $ 46.94万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-08-10 至 2018-01-31
  • 项目状态:
    已结题

项目摘要

Project Summary/Abstract Mental disease, including schizophrenia, depression and autism spectrum disorders, are still poorly understood, although it is clear that they mostly represent cortical disorders. The cortex is the primary site of higher mental functions, and despite extensive research, there is still no unified theory of how the cortex works. This is partly due to the fact that neuroscientists have traditionally relied on microelectrodes to record the activity of individual cells. However, cortical circuits are composed of millions of neurons and it is conceivable that single cell measurements alone will not be sufficient to unravel function of the brain. Optical imaging techniques tackle this emergent level of neuronal circuit activity and enable to image the activity of neuronal ensembles, in vitro and in vivo, while preserving single cell resolution, something that brain imaging techniques such as MRI or PET, cannot do. Moreover, the development of genetically encoded photosensitive proteins (optogenetics) and optochemical (caged) compounds offers the opportunity to not only image the activity of many neurons but also to optically control them. Advances in optogenetics has led to the development of two-photon microscopes combined with spatial light modulators (SLM), that allow the customer to split the laser beam into a holographic pattern that can be used to image activity or photoactivate neurons simultaneously in 3D. Multiple commercially available microscopes are now offered. Additionally researchers are customizing their own microscopes as a low cost alternative to purchasing new commercially available microscopes. The Meadowlark Optics (MLO) SLM is preferentially utilized due to the unprecedented response time a factor of 8 faster than competing SLMs, enabling more complex studies of brain function. However, the limited resolution of the SLM leads to studies over a smaller volume, and low fill factor leads to optical losses. To address these concerns MLO is proposing the development of a high speed (142 Hz to 500 Hz), high fill factor (95%), high resolution SLM. To reduce cost, and time to market MLO proposes to utilize an existing backplane, representing a total savings of $1M, and 1 year of development. Meadowlark will realize a prototype system within the Phase I, and will utilize internal funds to complete the product.
项目概要/摘要 包括精神分裂症、抑郁症和自闭症谱系障碍在内的精神疾病的治疗效果仍然很差 理解,尽管很明显它们主要代表皮质疾病。皮质是主要部位 更高的心理功能,尽管进行了广泛的研究,但仍然没有关于皮层如何工作的统一理论。 这部分是由于神经科学家传统上依靠微电极来记录 单个细胞的活动。然而,皮质回路是由数百万个神经元组成的,可以想象 仅单细胞测量不足以揭示大脑的功能。光学成像 技术解决了神经元回路活动的这种新兴水平,并能够对神经元的活动进行成像 体外和体内的整体,同时保留单细胞分辨率,这是大脑成像技术的特点 例如 MRI 或 PET,无法做到。此外,基因编码光敏蛋白的开发 (光遗传学)和光化学(笼式)化合物不仅提供了对以下物质的活动进行成像的机会 许多神经元,而且还可以对它们进行光学控制。 光遗传学的进步导致了双光子显微镜与空间显微镜相结合的发展 光调制器 (SLM),允许客户将激光束分割成全息图案,该图案可以 用于在 3D 中同时对活动进行成像或光激活神经元。多种市售 现在提供显微镜。此外,研究人员正在定制自己的显微镜,以降低成本 购买新的市售显微镜的替代方案。 Meadowlark Optics (MLO) SLM 是 由于前所未有的响应时间(比竞争对手的 SLM 快 8 倍)而得到优先利用, 能够对大脑功能进行更复杂的研究。然而,SLM 的有限分辨率导致研究 在较小的体积内,低填充因子会导致光学损耗。为了解决这些问题,MLO 提出 开发高速(142 Hz 至 500 Hz)、高填充因子(95%)、高分辨率的 SLM。减少 成本和上市时间 MLO 建议利用现有背板,总共节省 100 万美元, 和1年的开发。 Meadowlark 将在第一阶段实现原型系统,并将利用 内部资金来完成产品。

项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Anna Linnenberger其他文献

Anna Linnenberger的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Anna Linnenberger', 18)}}的其他基金

3D living neural networks
3D 活体神经网络
  • 批准号:
    8647789
  • 财政年份:
    2014
  • 资助金额:
    $ 46.94万
  • 项目类别:

相似海外基金

How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
  • 批准号:
    BB/Z514391/1
  • 财政年份:
    2024
  • 资助金额:
    $ 46.94万
  • 项目类别:
    Training Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
  • 批准号:
    2312555
  • 财政年份:
    2024
  • 资助金额:
    $ 46.94万
  • 项目类别:
    Standard Grant
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
  • 批准号:
    2327346
  • 财政年份:
    2024
  • 资助金额:
    $ 46.94万
  • 项目类别:
    Standard Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
  • 批准号:
    ES/Z502595/1
  • 财政年份:
    2024
  • 资助金额:
    $ 46.94万
  • 项目类别:
    Fellowship
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
  • 批准号:
    23K24936
  • 财政年份:
    2024
  • 资助金额:
    $ 46.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
  • 批准号:
    ES/Z000149/1
  • 财政年份:
    2024
  • 资助金额:
    $ 46.94万
  • 项目类别:
    Research Grant
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
  • 批准号:
    2901648
  • 财政年份:
    2024
  • 资助金额:
    $ 46.94万
  • 项目类别:
    Studentship
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
  • 批准号:
    488039
  • 财政年份:
    2023
  • 资助金额:
    $ 46.94万
  • 项目类别:
    Operating Grants
New Tendencies of French Film Theory: Representation, Body, Affect
法国电影理论新动向:再现、身体、情感
  • 批准号:
    23K00129
  • 财政年份:
    2023
  • 资助金额:
    $ 46.94万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The Protruding Void: Mystical Affect in Samuel Beckett's Prose
突出的虚空:塞缪尔·贝克特散文中的神秘影响
  • 批准号:
    2883985
  • 财政年份:
    2023
  • 资助金额:
    $ 46.94万
  • 项目类别:
    Studentship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了