Serial, non-invasive molecular analysis of exhaled breath condensate to define the pulmonary flora in critically injured, ventilated adults

 描述（由申请人提供）：这是一项创新提案，旨在确定呼出气冷凝液过滤器（EBCF）的分子分析能力，以预测创伤重症监护室患者的呼吸机相关性肺炎（VAP）。基于51例疑似VAP患者接受支气管肺泡灌洗的初步数据，我们之前已经证明了支气管肺泡灌洗和呼气冷凝液定量PCR之间的强相关性。本提案的目的是了解机械通气、危重受伤成人的肺部微生物群落发生的变化，以支持EBCF分子分析的发展，从而提供更早、更灵敏和更特异的VAP诊断，以实现：1）早期识别发展中的肺炎，2）靶向抗菌治疗，3）评估治疗反应。

 DESCRIPTION (provided by applicant): This is an innovative proposal to determine the capacity of molecular analysis of exhaled breath condensate filters (EBCF) to predict ventilator associated pneumonia (VAP) in trauma intensive care unit patients. Based upon preliminary data of 51 patients suspected of having VAP, who underwent bronchoalveolar lavage, we have previously demonstrated a strong correlation between bronchoalveolar lavage and breath condensate quantitative PCR. The goal of this proposal is to understand the changes that occur in the pulmonary microbial community of mechanically ventilated, critically injured adults to support the development of molecular analysis of EBCF to provide an earlier, more sensitive and specific diagnosis of VAP, to enable: 1) earlier recognition of a developing pneumonia, 2) targeted antimicrobial therapy, and 3) assessment of treatment response.