Serial, non-invasive molecular analysis of exhaled breath condensate to define the pulmonary flora in critically injured, ventilated adults

 描述(由申请人提供)：这是一项创新性的建议，旨在确定呼气冷凝液过滤器(EBCF)的分子分析能力，以预测创伤重症监护病房患者的呼吸机相关性肺炎(VAP)。根据51名怀疑患有VAP的患者的初步数据，他们接受了支气管肺泡灌洗，我们先前已经证明了支气管肺泡灌洗与呼吸冷凝物定量聚合酶链式反应之间存在强烈的相关性。这项建议的目的是了解机械通气危重损伤成人肺部微生物群落的变化，以支持EBCF分子分析的发展，从而提供VAP的更早、更敏感和更特异的诊断，从而能够：1)更早地识别发展中的肺炎，2)有针对性的抗菌治疗，3)治疗反应的评估。

 DESCRIPTION (provided by applicant): This is an innovative proposal to determine the capacity of molecular analysis of exhaled breath condensate filters (EBCF) to predict ventilator associated pneumonia (VAP) in trauma intensive care unit patients. Based upon preliminary data of 51 patients suspected of having VAP, who underwent bronchoalveolar lavage, we have previously demonstrated a strong correlation between bronchoalveolar lavage and breath condensate quantitative PCR. The goal of this proposal is to understand the changes that occur in the pulmonary microbial community of mechanically ventilated, critically injured adults to support the development of molecular analysis of EBCF to provide an earlier, more sensitive and specific diagnosis of VAP, to enable: 1) earlier recognition of a developing pneumonia, 2) targeted antimicrobial therapy, and 3) assessment of treatment response.