Serial, non-invasive molecular analysis of exhaled breath condensate to define the pulmonary flora in critically injured, ventilated adults

对呼出气冷凝物进行连续、非侵入性分子分析，以确定重伤、通气成人的肺部菌群

• 批准号: 8937571
• 负责人: Wonder P. Drake
• 金额: $ 31.01万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2016-04-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8937571
• 关键词: Accounting; Adult; Antibiotic Therapy; Antibiotics; Bacterial DNA; Bronchoalveolar Lavage; Bronchoalveolar Lavage Fluid; Chest; Clinical; Clinical Management; Communicable Diseases; Coughing; Critical Care; Critical Illness; DNA; Data; Development; Diagnosis; Early Diagnosis; Early identification; Environmental air flow; Equilibrium; Exhalation; Fever; Functional disorder; Goals; Growth; Healthcare; Heating; Hour; Immune; Immune response; Infection; Intensive Care Units; Length of Stay; Leukocytosis; Liquid substance; Lung; Manuscripts; Measures; Mechanical ventilation; Microbial Genetics; Molecular; Molecular Analysis; Morbidity - disease rate; Nature; Non-Invasive Cancer Detection; Outcome; Oxygen; Patients; Pneumonia; Predisposition; Procedures; Property; Pulmonary alveolar structure; Recombinant DNA; Regression Analysis; Relative (related person); Research; Resolution; Risk; Sampling; Severities; Signs and Symptoms; Specimen; Sputum; Symptoms; Testing; Therapeutic; Time; Trauma; Trauma patient; Tube; Universities; Virulence; Work; aerosolized; antimicrobial; base; clinical Diagnosis; cohort; cost; diagnosis standard; endotracheal; injured; innovation; microbial; microbial community; novel; pathogen; predictive modeling; public health relevance; response; statistics; targeted delivery; tool; treatment response; ventilator-associated pneumonia

 DESCRIPTION (provided by applicant): This is an innovative proposal to determine the capacity of molecular analysis of exhaled breath condensate filters (EBCF) to predict ventilator associated pneumonia (VAP) in trauma intensive care unit patients. Based upon preliminary data of 51 patients suspected of having VAP, who underwent bronchoalveolar lavage, we have previously demonstrated a strong correlation between bronchoalveolar lavage and breath condensate quantitative PCR. The goal of this proposal is to understand the changes that occur in the pulmonary microbial community of mechanically ventilated, critically injured adults to support the development of molecular analysis of EBCF to provide an earlier, more sensitive and specific diagnosis of VAP, to enable: 1) earlier recognition of a developing pneumonia, 2) targeted antimicrobial therapy, and 3) assessment of treatment response.