Characterization of a novel quorum quenching protein produced by S aureus

金黄色葡萄球菌产生的新型群体猝灭蛋白的表征

• 批准号: 9113500
• 负责人: Michael Eric Olson
• 金额: $ 10.74万
• 依托单位: SOUTHERN ILLINOIS UNIVERSITY SCH OF MED
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-20 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9113500
• 关键词: Acute; Adult; Antibiotic Resistance; Bacteria; Bacterial Physiology; Binding; Biological; Cessation of life; Chronic; Clinical; Community Hospitals; Cotton Rats; Data; Dependence; Disease; Down-Regulation; Environment; Enzymes; Genetic; Genetic Transcription; Genus staphylococcus; Gram-Positive Bacteria; Growth; Health; Healthcare; Homologous Gene; Human; Human Microbiome; Immunoblotting; Individual; Infection; Infection prevention; Iowa; Knowledge; Laboratories; Lead; Microbial Biofilms; Modeling; Molecular; Morbidity - disease rate; Nasal Epithelium; Natural regeneration; Nature; Nose; Nosocomial Infections; Paper; Pathogenesis; Peptide Hydrolases; Pharyngeal structure; Physiology; Positioning Attribute; Postdoctoral Fellow; Production; Property; Protein S; Proteins; Public Health; Rectum; Regulation; Regulatory Element; Research; Research Institute; Research Personnel; Role; Site; Skin; Staphylococcus aureus; Staphylococcus epidermidis; Surveys; System; Tissues; Toxin; Universities; Upper respiratory tract; Vagina; Work; experience; fitness; improved; insight; interest; microorganism interaction; mortality; new therapeutic target; novel; novel therapeutics; pathogen; prevent; quorum sensing; receptor; research study; resistant strain; tenure track

DESCRIPTION (provided by applicant): The principle investigator, (Dr. Olson), is currently a postdoctoral fellow at the University of Iowa. He has significant experience studying bacterial physiology, biofilm production and host colonization factors. Within the next 12 months, he plans on obtaining a tenure track academic position at a major research institute with a future research focus on understanding S. aureus physiology and pathogenesis. The long-term objectives of his laboratory will be to understand the molecular mechanisms involved in S. aureus colonization. His research interests involve understanding how the staphylococci interact with each other and with the host. The main project that he is pursuing involves the characterization of a novel quorum-quenching protein that S. aureus secretes. Current work involves elucidating the mechanism by which S. aureus is able to disrupt and alter genetic function of other bacteria, specifically, S. epidermidis. Overall, the understanding polymicrobial interactions and the effects of bacteria have on each other will be used to gain insight into both human colonization and the dynamics of the human microbiome. The specific aims of this proposal are: I) determine the extent, breadth and distribution of the quorum quenching protein, SqqA; II) define the mechanism by which SqqA interferes with S. epidermidis quorum sensing; and III) assess how this polymicrobial interplay modulates the colonization state of the human host. Dr. Olson's research plan has great relevance to public health as S. aureus is a major cause of infection and death in community and hospital associated infections. Findings from the proposed project will increase understanding of S. aureus colonization mechanisms and may lead to new therapeutic options for preventing S. aureus infections.

描述（由申请人提供）：主要研究者（Olson博士）目前是爱荷华州大学的博士后研究员。他在研究细菌生理学、生物膜产生和宿主定植因素方面具有丰富的经验。在接下来的12个月里，他计划在一家主要的研究机构获得终身教职，未来的研究重点是了解S。金黄色葡萄球菌生理学和发病机制。他的实验室的长期目标将是了解参与S。金黄色葡萄球菌定植。他的研究兴趣包括了解葡萄球菌如何相互作用以及与宿主的相互作用。他正在进行的主要项目涉及一种新的群体淬灭蛋白的特征，这种蛋白是S。金黄色葡萄球菌分泌。目前的工作包括阐明S。金黄色葡萄球菌能够破坏和改变其他细菌的遗传功能，特别是S。表皮总的来说，了解多微生物相互作用和细菌对彼此的影响将用于深入了解人类定植和人类微生物组的动态。这个建议的具体目标是：I）确定群体淬灭蛋白，SqqA的程度，广度和分布; II）定义SqqA干扰S的机制。III）评估这种多微生物相互作用如何调节人类宿主的定殖状态。奥尔森博士的研究计划与公共卫生有很大的相关性，因为S。金黄色葡萄球菌是社区和医院相关感染中感染和死亡的主要原因。该项目的研究结果将增加对S.金黄色葡萄球菌定植机制，并可能导致新的治疗选择，以防止S。金黄色葡萄球菌感染