20(OH) Vit D3, T Cells, and Arthritis

20(OH) 维生素 D3、T 细胞和关节炎

• 批准号: 9036329
• 负责人: ANDREW H KANG
• 金额: $ 33万
• 依托单位: UNIVERSITY OF TENNESSEE HEALTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9036329
• 关键词: Affect; Animal Model; Arthritis; Attenuated; Autoimmune Diseases; Biochemical; CTLA4-Ig; Cell Lineage; Cells; Cholecalciferol; Collagen Type II; Collagen-Induced Arthritis; Confocal Microscopy; DBA/1 Mouse; Development; Disease; Distal; Dose; Down-Regulation; Effectiveness; Enzyme-Linked Immunosorbent Assay; Etiology; Flow Cytometry; Frequencies; Genetic Transcription; Histology; Human; Immunization; Immunodominant Epitopes; Immunosuppressive Agents; Inflammatory; Inflammatory Response; Interferons; Interleukin-10; Interleukin-17; Interleukin-4; Intervention; Joints; Kinetics; Ligands; Location; Lymphoid; MAPK14 gene; MAPK8 gene; Methods; Microfluidics; Mitogen-Activated Protein Kinases; Modality; Modeling; Molecular; Mus; Pathogenesis; Pathway interactions; Pattern; Peptides; Peripheral Blood Mononuclear Cell; Phenotype; Production; Regulatory T-Lymphocyte; Reporter; Reverse Transcriptase Polymerase Chain Reaction; Rheumatoid Arthritis; Risk; Role; Severities; Signal Pathway; Signal Transduction; Staining method; Stains; T-Cell Activation; T-Lymphocyte; Testing; Therapeutic; Up-Regulation; Vitamin D; Vitamin D Analog; Vitamin D Deficiency; Vitamin D supplementation; Vitamin D3 Receptor; Western Blotting; Work; analog; autoimmune arthritis; cytokine; effective therapy; inhibitor/antagonist; peptide analog; public health relevance; research study; response; success; transcription factor; treatment response; treatment strategy

DESCRIPTION (provided by applicant): Studies citing vitamin D deficiency associated with rheumatoid arthritis (RA) have indicated that vitamin D supplementation might reduce the risk of or enhance therapeutic approaches to treat this disease. The discovery of a new secosteroidogenic pathway initiated by P450scc that produces vitamin D3 hydroxyderivatives has opened new options in treatment for RA. This first and main product of the pathway, 20(OH)D3, is non-toxic at concentrations as high as 30?g/kg. We have approached the molecular pathogenesis of RA under the thesis that RA is an autoimmune disorder characterized by T cell dysregulation and that the use of vitamin D analogs can enhance the immunomodulatory effects of T cells. For example, T cells activated by altered peptide ligand (APL) peptide of the immunodominant epitope of type II collagen (CII), ie A9, CII245-270 (A260, B261, N263), upregulate the expression of the vitamin D receptor (VDR) so that both 1,25(OH)2D3 and 20(OH)D3 enhance the suppression of the inflammatory responses by inhibiting Th1 and Th17 responses while increasing the production of Th2 cytokines and IL- 10. We feel that the net result of combining therapies with both analog peptides and vitamin D will be a synergistic downregulation of the severity of arthritis at lower and safer doses than either therapy alone. Yet the mechanism of this suppression remains unknown. Our central hypothesis is that T inhibitory cells induced by the APL upregulate the Vitamin D receptor (VDR) allowing vitamin D3 to act directly on T cells to enhance both the suppression of inflammatory cytokines and the secretion of Th2/regulatory cytokines ultimately leading to suppression of autoimmune arthritis; and that the noncalcemic 20(OH)D3, will be as effective and less toxic than the classical form of vitamin D3 [1,25(OH) 2D3]. To understand the mechanism by which vitamin D enhances this response we propose the following specific aims: Aim 1) To test the hypothesis that redirecting the T cell cytokine profile is a mechanism by which 20(OH)D3 enhances the effectiveness of an APL in attenuating collagen-induced arthritis, Aim 2) To test the hypothesis that modulation of autoimmune arthritis can be enhanced by combining two interventions[ 20(OH)D3 and an APL], and Aim 3) To test the hypothesis that 20(OH)D3 intersects the alternate T cell signaling pathway and affects APL driven T cell cytokine production. Successful completion of these experiments will demonstrate if vitamin D and analog peptides work in synergy to suppress autoimmune arthritis and whether the noncalcemic form of vitamin D, 20(OH)D3 is as effective as 1,25(OH)2D3.

描述（由申请人提供）：引用与类风湿性关节炎（RA）相关的维生素D缺乏症的研究表明，维生素D补充剂可能会降低这种疾病的风险或增强治疗方法。由P450 SCC启动的产生维生素D3羟基衍生物的新开环类固醇生成途径的发现为RA的治疗开辟了新的选择。这第一个和主要产品的途径，20（OH）D3，是无毒的浓度高达30？g/kg。我们探讨了RA的分子发病机制，认为RA是一种自身免疫性疾病，其特征是T细胞失调，使用维生素D类似物可以增强T细胞的免疫调节作用。例如，由II型胶原蛋白（CII）的免疫显性表位（即A9，CII 245 -270）的改变的肽配体（APL）肽激活的T细胞（A260，B261，N263），上调维生素D受体（VDR）的表达，使得1，25（OH）2D 3和20（OH）D3通过抑制Th 1和Th 17应答同时增加Th 2细胞因子和IL- 10的产生来增强对炎症应答的抑制。我们认为，将类似肽和维生素D联合治疗的净结果将是以比单独治疗更低和更安全的剂量协同下调关节炎的严重程度。然而，这种抑制的机制仍然未知。我们的中心假设是由APL诱导的T抑制性细胞上调维生素D受体（VDR），使维生素D3直接作用于T细胞，以增强对炎性细胞因子的抑制和Th 2/调节细胞因子的分泌，最终导致自身免疫性关节炎的抑制;并且非钙离子20（OH）D3将与维生素D3的经典形式[1，25（OH）2D 3]一样有效且毒性更小。为了了解维生素D增强这种反应的机制，我们提出了以下具体目标：目的1）为了验证以下假设：重定向T细胞细胞因子谱是20（OH）D3增强APL在减轻胶原诱导的关节炎中的有效性的机制，目的2）为了检验通过结合两种干预[ 20（OH）D3和APL]可以增强自身免疫性关节炎的调节的假设，目的3）验证20（OH）D3与T细胞交替信号通路交叉并影响APL驱动的T细胞细胞因子产生的假说。这些实验的成功完成将证明维生素D和类似肽是否协同作用以抑制自身免疫性关节炎，以及维生素D的非钙形式20（OH）D3是否与1，25（OH）2D 3一样有效。