Cytometry by Time of Fight (CyTOF) for High-speed Single-cell Analysis

用于高速单细胞分析的战斗时间细胞计数 (CyTOF)

• 批准号: 9075728
• 负责人: Fei Wen
• 金额: $ 60万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-04-01 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075728
• 关键词: 6 year old; Address; Advisory Committees; Antibodies; Area; Award; Bioinformatics; Biological; Biology; Biomedical Engineering; Cancer Biology; Cells; Clinical Research; Complex; Cytometry; Detection; Elements; Ensure; Equipment; Funding; Goals; Health; Immunologic Monitoring; Immunology; Label; Mass Spectrum Analysis; Metals; Michigan; Neurology; Pathology; Population; Research; Research Activity; Research Infrastructure; Research Personnel; Schools; Speed; System; Technology; Therapeutic; Time; Training; United States National Institutes of Health; Universities; base; bone; college; experience; fighting; fundamental research; improved; instrument; operation; pre-clinical; programs; ranpirnase; research study; single cell analysis

 DESCRIPTION (provided by applicant): The objective of this proposal is to request $600,000 to purchase a CyTOF, a mass-spectrometry-based cytometer, for multi-investigator use at the University of Michigan. CyTOF is a cutting edge technology only ~6 years old. There are ~50 CyTOF instruments available in the US, and currently there is none available in the entire State of Michigan. The key feature of CyTOF is the use of metal element-tagged antibodies that enables detection of up to 100 markers on a single cell, as compared to ~12 parameters for the staple flow cytometery technology that uses fluorescently labeled antibodies. This exquisite multiplexed detection capability of the CyTOF enables a much more comprehensive assessment of complex biological/immunological systems with both breadth (detect many cell populations at once) and depth (detect many markers on each of the populations). This systematic approach is critical for advancing our understanding of these complex systems, for expanding the questions that can be addressed in multiple investigative directions, and for achieving fundamental and clinical research goals in many fields. As such, this proposal supports a group of 24 investigators from 10 Departments in 4 Schools and Colleges at the University of Michigan, who see the great potential of the use of CyTOF in accelerating and advancing their research programs. These 24 majors users have signed up for 85% of the equipment time for experiments in 28 R01s, 5 R21s, 4 R03s, one project in P01, two projects in P30, three projects in U19, one NIH Director's Award, one U54 and two R56 awards, a total of 47 NIH-funded projects. These projects are directly related to NIH goals of improving health and include studies in diverse areas of cancer biology and therapeutics, pathology, bone biology, immunology, neurology, immuno-bioengineering, etc. Thus, this proposal will clearly support the infrastructure of NIH-funded research at the University of Michigan, and represent an important endeavor to establish the state-of-the-art facility for complex cellular network studies. The CyTOF will be supported by the Immunologic Monitoring Core (IMC), experienced technical staff (1.0 FTE), bioinformatics experts, an Internal Advisory Committee, and the PI, who has extensive research and technical experience with the CyTOF. A system to attract and train new users is in place to ensure accessibility to other NIH-funded investigators. Strong institutional supports have been committed to the space, acquisition ($30,000), and long-term operation (a total of $119,500 per year for a period of four years) of the CyTOF. A user-driven recharge system will be set up by the IMC for the continued long-term use of the CyTOF. In summary, availability of the CyTOF at the University of Michigan will clearly have a broad impact on the basic, preclinical, and translational NIH-funded research activities of many investigators (24 already identified); and we have strong infrastructural, administrative, fiscal, technical, and dat supports for establishing a successful and vibrant CyTOF program.

 描述(由申请人提供)：这项提案的目标是要求600,000美元购买一台CyTOF，一种基于质谱学的细胞仪，用于密歇根大学的多名调查员使用。CyTOF是一项仅有6年历史的尖端技术。美国有大约50种CyTOF仪器，但目前整个密歇根州还没有一种。CyTOF的主要特点是使用金属元素标记的抗体，能够在单个细胞上检测多达100个标记物，而使用荧光标记抗体的主要流式细胞术技术只有~12个参数。CyTOF的这种精致的多重检测能力使我们能够更全面地评估复杂的生物/免疫系统，包括广度(同时检测多个细胞群体)和深度(检测每个群体上的多个标记)。这种系统化的方法对于促进我们对这些复杂系统的理解，对于扩大可以在多个研究方向上解决的问题，以及在许多领域实现基础和临床研究目标至关重要。因此，这项提案支持一个由来自密歇根大学4所学院10个系的24名研究人员组成的小组，他们看到了使用CyTOF加速和推进他们的研究计划的巨大潜力。这24个专业用户已在28个R01、5个R21、4个R03、P01 1个项目、P30 2个项目、U19 3个项目、1个NIH主任奖、1个U54和2个R56奖项中签约了85%的设备时间，共计47个NIH资助项目。这些项目与NIH改善健康的目标直接相关，包括癌症生物学和治疗学、病理学、骨生物学、免疫学、神经学、免疫生物工程等多个领域的研究。因此，这项提议将明显支持NIH资助的密歇根大学研究的基础设施，并代表着为建立复杂细胞网络研究的最先进设施所做的重要努力。免疫监测核心(IMC)、经验丰富的技术人员(1.0FTE)、生物信息学专家、内部咨询委员会以及对细胞周期监测具有广泛研究和技术经验的PI将为细胞周期监测提供支持。一个吸引和培训新用户的系统已经到位，以确保其他由NIH资助的调查人员能够接触到。一直致力于提供强有力的机构支助，以支持该基金的空间、购置(30,000美元)和长期运作(为期四年，每年总共119,500美元)。IMC将建立一个由用户驱动的充值系统，以继续长期使用CyTOF。综上所述，在密歇根大学提供CyTOF显然将对许多研究人员(已确定的24人)的基础、临床前和转化性研究活动产生广泛影响；我们拥有强大的基础设施、管理、财政、技术和DAT支持，可以建立一个成功和充满活力的CyTOF计划。