Food safe pathogen surrogates based on encapsulated short DNA sequences for rapid verification of sanitation in food processing

基于封装短 DNA 序列的食品安全病原体替代物，用于快速验证食品加工中的卫生状况

• 批准号: 9464652
• 负责人: Laurie Marie Clotilde
• 金额: $ 53.53万
• 依托单位: SAFETRACES, INC.
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-15 至 2019-10-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9464652
• 关键词: Food; safe; pathogen; surrogates; based

Food Safe Pathogen Surrogates for Rapid Verification of Sanitation in Food Processing Phase II Project Summary Food safety is a major issue with global impact. The CDC estimates that in the U.S. alone, 3,000 deaths are caused each year due to foodborne illnesses. It is estimated that a total of 76 million food-related illness cases occurred in 2013, with the U.S. spending $40 billion on treatment rather than prevention (Food Quality and Safety Magazine, 2015). Even though foodborne illnesses are often attributed to meat, dairy, and seafood, outbreaks due to contaminated produce have been on the rise (Harris et al., 2003; Lynch et al., 2009; Callejòn et al., 2015). Fresh fruits and vegetables are mostly grown outdoors, leaving them susceptible to potential environmental contamination. Washing is the primary method employed by processors to ensure the safety of the produce they supply. However, washing is not always effective in removing microbial contaminants (decontamination). If the wash system is improperly managed, cross contamination can covert a small contamination event into an outbreak event. SafeTraces is proposing the development of SafeTracers™, a reliable, repeatable, continuous, fast, on-site test performed on finished product, to ensure appropriate washing by assessing decontamination and risk of cross-contamination of fresh produce. SafeTracers™ are edible (Generally Recognized As Safe) microparticles, tagged with non-living, non-viable DNA, that behave like pathogens under wash conditions. They will enable validation, verification, and continuous monitoring of decontamination and cross-contamination in wash systems. SafeTracers™ will be easily retrofitted on any wash system, and will be robust and simple to operate. During Phase II, we will compare SafeTracers™ to prominent pathogens for their attachment, detachment, transfer, and inactivation on abiotic (glass and stainless steel) and on produce (spinach) surfaces. We will develop a simple workflow to sample finished product, measure SafeTracers™ levels, and assess the effectiveness of the wash process, all in less than 30 minutes. Furthermore, we will develop a SafeTracers™-based method to assess the risk of E. coli cross contamination for leafy produce commodities in both one-pass and flume wash systems using chlorinated water wash. Finally, we will complete validation studies for the two SafeTracers™-based methods aimed at assessing the risks for insufficient decontamination and cross contamination in wash systems operating under validated conditions and processing leafy greens.

食品安全病原体替代物用于食品加工卫生快速验证 二期 项目摘要 食品安全是具有全球影响的重大问题。CDC估计，仅在美国， 每年有3,000人死于食源性疾病。据估计，共有76名 2013年发生了100万例与食物有关的疾病，美国在治疗上花费了400亿美元 而不是预防（食品质量和安全杂志，2015）。尽管食源性 疾病通常归因于肉类、奶制品和海鲜， 一直在上升（Harris等人，2003; Lynch等人，2009; Callejn等人，2015年）的报告。 新鲜水果和蔬菜大多在户外种植， 潜在的环境污染。洗涤是加工商采用的主要方法 以确保他们供应的产品的安全。然而，洗涤并不总是有效的， 去除微生物污染物（净化）。如果洗涤系统管理不当， 交叉污染可以将小的污染事件转变为爆发事件。 SafeTraces建议开发SafeTracers™，这是一种可靠、可重复、 对成品进行连续、快速、现场测试，以确保适当的清洗， 评估新鲜农产品的去污和交叉污染风险。SafeTracers™是 可食用的（通常认为是安全的）微粒，用无生命的、无活力的DNA标记， 在洗涤条件下表现得像病原体。它们将使验证、核实和 持续监测清洗系统中的去污和交叉污染。 SafeTracers™可在任何清洗系统上轻松改装，并且坚固耐用，操作简单。 手术。 在第二阶段，我们将比较SafeTracers™与主要病原体的 在非生物（玻璃和不锈钢）和非生物（玻璃和不锈钢）上的附着、分离、转移和灭活 产生（菠菜）表面。我们将开发一个简单的工作流程来对成品进行采样， 测量SafeTracers™水平，并评估洗涤过程的有效性，所有这些都在不到 30分钟此外，我们将开发一种基于SafeTracers™的方法来评估E. 在单程和水槽洗涤系统中，叶类农产品的大肠杆菌交叉污染 使用氯化水洗涤。最后，我们将完成对这两个项目的验证研究。 基于SafeTracers™的方法旨在评估去污不足的风险， 在经验证的条件下运行的清洗系统中的交叉污染， 绿色。