Investigation of chitosan-supported silver as a safe and effective broad-spectrum antimicrobial textile finish

壳聚糖负载银作为安全有效的广谱抗菌纺织品整理剂的研究

• 批准号: 10825963
• 负责人: Dana Oliver
• 金额: $ 5.5万
• 依托单位: CHITOZAN HEALTH, LLC
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-12 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10825963
• 关键词: Affect; Amines; Antifungal Agents; Beds; Binding; Chitosan; Clothing; Color; Contact Inhibition; Data; Development; Digestion; Disinfection; Effectiveness; Event; Formulation; Foundations; Goals; Gossypium; Gram-Negative Bacteria; Gram-Positive Bacteria; Health; Healthcare; Hospitals; Inductively Coupled Plasma Mass Spectrometry; Investigation; Measurement; Medical Care Costs; Methods; Minor; Molds; Nitric Acid; Nosocomial Infections; Operative Surgical Procedures; Optics; Organism; Outcome; Patients; Polyesters; Powder dose form; Privacy; Procedures; Process; Production; Property; Research; Sampling; Silver; Testing; Text; Textiles; Universities; Water; Weight; Work; Yeasts; antimicrobial; antimicrobial drug; combat; crosslink; innovation; manufacture; pathogen; prevent; transmission process; vector; vector transmission

PROJECT SUMMARY Maximum of 30 lines of text Minimum of ½” margins Font size 11 pts or greater The overall research goal of the proposed work is to test the antimicrobial activity and durability of a chitosan-supported silver-based textile finish for incorporation into hospital privacy curtains. Furthermore, successful development of this textile finish for privacy curtains would set the foundation for development of additional antimicrobial textiles for incorporation in bed sheets, blankets, patient clothing, scrubs, and surgical sheets, for example. Antimicrobial textiles serve to combat hospital-acquired infections which affect approximately 2 million patients per year and incur annual direct medical costs of at least $28.4 billion in the U.S. In support of the overall research goal, Specific Aim 1 will determine the loading rate of the chitosan silver product on a variety of textiles relevant to the healthcare field given a manufacturing tolerance of 10%. The current chitosan silver product (1% chitosan and 0.15% silver) will be manufactured with a tolerance of 10% (0.9-1.1% chitosan and 0.14-0.16% silver) and used to finish cotton, polyester, spandex, and textile blends, with subsequent determination of the loading rate of the chitosan silver textile finish. Results of Specific Aim 1 will provde the basis for correlating antimicrobial activity with chitosan and silver concentrations on treated textiles. Specific Aim 2 will determine the antimicrobial activity of the textiles prepared in Specific Aim 1. Four organisms will be used in testing: a gram negative bacterium, a gram positive bacterium, a yeast, and a mold. Testing will determine the presence of diffusible antimicrobial agents, indicative of leaching of the chitosan silver finish, and further testing will determine contact inhibition of the treated textiles. Results of Specific Aim 2 will determine the primary mode of antimicrobial activity, which is anticipated to be via contact inhibition rather than leaching of the chitosan silver finish. Specific Aim 3 will demonstrate the durability of the textile finish during washing and disinfection. Textiles will be washed, with and without bleach, using typical cleaning conditions for healthcare textiles. Antimicrobial efficacy will be tested after 10, 50, and 100 washes using the same methods as described for Specific Aim 2. After each washing stage, silver remaining on the textile will be determined via nitric acid digestion and ICP-MS. Results of Specific Aim 3 will demonstrate the durability of the textile finish during typical cleaning procedures, and verify the retention of the chitosan silver finish. Specific Aim 4 is to determine how the chitosan silver textile finish alters textile properties, specifically, color, water repellency, and flammability. The treated textiles are anticipated to demonstrate minimal graying, no impact on water repellency, and reduced flammability. To demonstrate the utility of this chitosan silver finish for preparing antimicrobial textiles, the anticipated outcome is at least a 3-log reduction for all pathogens for all treated textiles, both unwashed and washed up to 50 times.

项目摘要 最多30行文本 最小半”边距 字体大小11磅或以上 所提出的工作的总体研究目标是测试壳聚糖支撑的抗微生物活性和耐久性。 用于结合到医院隐私窗帘中的基于银的纺织品整理。此外，成功开发这一 隐私窗帘的纺织品整理将为开发其他抗菌纺织品奠定基础， 例如，可以将其结合到床单、毯子、病人衣服、手术服和手术床单中。抗菌纺织品服务 防治每年影响约200万患者并导致每年直接 在美国，医疗费用至少为284亿美元。为了支持总体研究目标，具体目标1将确定 壳聚糖银产品在与保健领域相关的各种纺织品上的负载率 公差为10%。当前壳聚糖银产品（1%壳聚糖和0.15%银）将在公差范围内生产 10%（0.9-1.1%壳聚糖和0.14-0.16%银），用于整理棉、聚酯、涤纶和纺织品混纺织物， 随后测定脱乙酰壳多糖银织物整理剂的负载率。具体目标1的结果将证明 将抗微生物活性与经处理的纺织品上的壳聚糖和银浓度相关联的基础。具体目标2将 确定在具体目标1中制备的纺织品的抗微生物活性。试验中将使用四种微生物：a 革兰氏阴性菌、革兰氏阳性菌、酵母菌和霉菌。测试将确定是否存在可扩散的 抗菌剂，指示脱乙酰壳多糖银整理的浸出，并且进一步测试将确定接触抑制 经过处理的纺织品。特定目标2的结果将确定抗菌活性的主要模式，即 预期是通过接触抑制而不是脱乙酰壳多糖银整理剂的浸出。第3章将证明 在洗涤和消毒过程中织物整理的耐久性。纺织品将被洗涤，有或没有漂白剂，使用 卫生保健纺织品的典型清洁条件。将在使用10次、50次和100次洗涤后测试抗菌功效 与具体目标2所述的方法相同。在每个洗涤阶段之后，织物上残留的银将被 具体目标3的结果将证明纺织品的耐久性 在典型的清洁过程中完成，并验证壳聚糖银饰面的保留。具体目标4是确定 壳聚糖银织物整理剂如何改变织物性能，特别是颜色、防水性和易燃性。的 预期经处理的纺织品表现出最小的变灰、对防水性没有影响和降低的可燃性。 为了证明这种壳聚糖银整理剂用于制备抗菌纺织品的实用性，预期的结果是在 对于所有处理过的纺织品，无论是未洗涤的还是洗涤多达50次的，所有病原体都至少减少3个对数。