Investigation of chitosan-supported silver as a safe and effective broad-spectrum antimicrobial textile finish

壳聚糖负载银作为安全有效的广谱抗菌纺织品整理剂的研究

• 批准号: 10825963
• 负责人: Dana Oliver
• 金额: $ 5.5万
• 依托单位: CHITOZAN HEALTH, LLC
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-12 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10825963
• 关键词: Affect; Amines; Antifungal Agents; Beds; Binding; Chitosan; Clothing; Color; Contact Inhibition; Data; Development; Digestion; Disinfection; Effectiveness; Event; Formulation; Foundations; Goals; Gossypium; Gram-Negative Bacteria; Gram-Positive Bacteria; Health; Healthcare; Hospitals; Inductively Coupled Plasma Mass Spectrometry; Investigation; Measurement; Medical Care Costs; Methods; Minor; Molds; Nitric Acid; Nosocomial Infections; Operative Surgical Procedures; Optics; Organism; Outcome; Patients; Polyesters; Powder dose form; Privacy; Procedures; Process; Production; Property; Research; Sampling; Silver; Testing; Text; Textiles; Universities; Water; Weight; Work; Yeasts; antimicrobial; antimicrobial drug; combat; crosslink; innovation; manufacture; pathogen; prevent; transmission process; vector; vector transmission

PROJECT SUMMARY Maximum of 30 lines of text Minimum of ½” margins Font size 11 pts or greater The overall research goal of the proposed work is to test the antimicrobial activity and durability of a chitosan-supported silver-based textile finish for incorporation into hospital privacy curtains. Furthermore, successful development of this textile finish for privacy curtains would set the foundation for development of additional antimicrobial textiles for incorporation in bed sheets, blankets, patient clothing, scrubs, and surgical sheets, for example. Antimicrobial textiles serve to combat hospital-acquired infections which affect approximately 2 million patients per year and incur annual direct medical costs of at least $28.4 billion in the U.S. In support of the overall research goal, Specific Aim 1 will determine the loading rate of the chitosan silver product on a variety of textiles relevant to the healthcare field given a manufacturing tolerance of 10%. The current chitosan silver product (1% chitosan and 0.15% silver) will be manufactured with a tolerance of 10% (0.9-1.1% chitosan and 0.14-0.16% silver) and used to finish cotton, polyester, spandex, and textile blends, with subsequent determination of the loading rate of the chitosan silver textile finish. Results of Specific Aim 1 will provde the basis for correlating antimicrobial activity with chitosan and silver concentrations on treated textiles. Specific Aim 2 will determine the antimicrobial activity of the textiles prepared in Specific Aim 1. Four organisms will be used in testing: a gram negative bacterium, a gram positive bacterium, a yeast, and a mold. Testing will determine the presence of diffusible antimicrobial agents, indicative of leaching of the chitosan silver finish, and further testing will determine contact inhibition of the treated textiles. Results of Specific Aim 2 will determine the primary mode of antimicrobial activity, which is anticipated to be via contact inhibition rather than leaching of the chitosan silver finish. Specific Aim 3 will demonstrate the durability of the textile finish during washing and disinfection. Textiles will be washed, with and without bleach, using typical cleaning conditions for healthcare textiles. Antimicrobial efficacy will be tested after 10, 50, and 100 washes using the same methods as described for Specific Aim 2. After each washing stage, silver remaining on the textile will be determined via nitric acid digestion and ICP-MS. Results of Specific Aim 3 will demonstrate the durability of the textile finish during typical cleaning procedures, and verify the retention of the chitosan silver finish. Specific Aim 4 is to determine how the chitosan silver textile finish alters textile properties, specifically, color, water repellency, and flammability. The treated textiles are anticipated to demonstrate minimal graying, no impact on water repellency, and reduced flammability. To demonstrate the utility of this chitosan silver finish for preparing antimicrobial textiles, the anticipated outcome is at least a 3-log reduction for all pathogens for all treated textiles, both unwashed and washed up to 50 times.

项目摘要 最多30行文字 最低½”边距 字体尺寸11分或更高 拟议工作的总体研究目标是测试壳聚糖支持的抗菌活性和耐用性 用于保险的银色纺织品进入医院的隐私窗帘。此外，成功发展 隐私窗帘的纺织品饰面将为开发其他抗菌纺织品的基础 例如，在床单，毯子，耐心的衣服，磨砂膏和手术床单中掺入。抗菌纺织品服务 为了打击每年影响约200万患者并导致年度直接感染的医院获得感染 美国以支持总体研究目标的医疗费用至少为284亿美元，具体AIM 1将确定 鉴于制造业 公差为10％。当前的壳聚糖银产品（1％的壳聚糖和0.15％的银）将以公差制造 有10％（0.9-1.1％的壳聚糖和0.14-0.16％银），用于完成棉花，聚酯，氨纶和纺织品混合物， 随后确定壳聚糖银色纺织品饰面的加载速率。特定目标1的结果将导致 将抗菌活性与壳聚糖和银浓度相关的基础。具体目标2将 确定在特定目标1中制备的纺织品的抗菌活性。测试将使用四种生物：A 革兰氏阴性细菌，革兰氏阳性细菌，酵母和霉菌。测试将确定存在的存在 抗菌剂，指示壳聚糖银色饰面行走，进一步测试将确定接触抑制 处理的纺织品。特定目标2的结果将确定抗菌活性的主要模式，即 预计会通过接触抑制而不是提起壳聚糖银色饰面。特定目标3将证明 洗涤和消毒期间纺织品饰面的耐用性。纺织品将在没有漂白的情况下洗涤 医疗保健纺织品的典型清洁条件。使用10、50和100洗涤后，将测试抗菌效率 与特定目标2所述的方法相同的方法。每个洗涤阶段之后，纺织品上保留的银将是 通过硝酸消化和ICP-MS确定。特定目标3的结果将证明纺织品的耐用性 在典型的清洁过程中完成，并验证壳聚糖银色饰面的保留。特定目标4是确定 壳聚糖银色纺织品如何改变纺织特性，具体来说，颜色，驱动性和易燃性。这 预计经过处理的纺织品将证明最少的灰色，对水的驱排量没有影响，并且易燃性降低。 为了证明这种壳聚糖银色表面效用以准备抗菌纺织品，预期的结果是 对于所有处理过的纺织品，所有病原体的所有病原体都至少减少3型病原体，这些纺织品的未洗和洗涤高达50次。