Investigation of chitosan-supported silver as a safe and effective broad-spectrum antimicrobial textile finish

壳聚糖负载银作为安全有效的广谱抗菌纺织品整理剂的研究

• 批准号: 10546228
• 负责人: Dana Oliver
• 金额: $ 25.42万
• 依托单位: CHITOZAN HEALTH, LLC
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-12 至 2023-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10546228
• 关键词: Affect; Amines; Antifungal Agents; Beds; Binding; Centers for Disease Control and Prevention (U.S.); Chitosan; Clothing; Color; Contact Inhibition; Data; Development; Diffuse; Digestion; Disinfection; Effectiveness; Event; Formulation; Foundations; Goals; Gossypium; Gram-Negative Bacteria; Gram-Positive Bacteria; Health; Healthcare; Hospitals; Inductively Coupled Plasma Mass Spectrometry; Investigation; Measurement; Medical Care Costs; Methods; Minor; Molds; Nitric Acid; Nosocomial Infections; Operative Surgical Procedures; Optics; Organism; Outcome; Patients; Polyesters; Powder dose form; Privacy; Procedures; Process; Production; Property; Research; Sampling; Silver; Testing; Text; Textiles; Time; Universities; Water; Weight; Work; Yeasts; antimicrobial; antimicrobial drug; base; combat; crosslink; innovation; pathogen; prevent; transmission process; vector; vector transmission

PROJECT SUMMARY Maximum of 30 lines of text Minimum of ½” margins Font size 11 pts or greater The overall research goal of the proposed work is to test the antimicrobial activity and durability of a chitosan-supported silver-based textile finish for incorporation into hospital privacy curtains. Furthermore, successful development of this textile finish for privacy curtains would set the foundation for development of additional antimicrobial textiles for incorporation in bed sheets, blankets, patient clothing, scrubs, and surgical sheets, for example. Antimicrobial textiles serve to combat hospital-acquired infections which affect approximately 2 million patients per year and incur annual direct medical costs of at least $28.4 billion in the U.S. In support of the overall research goal, Specific Aim 1 will determine the loading rate of the chitosan silver product on a variety of textiles relevant to the healthcare field given a manufacturing tolerance of 10%. The current chitosan silver product (1% chitosan and 0.15% silver) will be manufactured with a tolerance of 10% (0.9-1.1% chitosan and 0.14-0.16% silver) and used to finish cotton, polyester, spandex, and textile blends, with subsequent determination of the loading rate of the chitosan silver textile finish. Results of Specific Aim 1 will provde the basis for correlating antimicrobial activity with chitosan and silver concentrations on treated textiles. Specific Aim 2 will determine the antimicrobial activity of the textiles prepared in Specific Aim 1. Four organisms will be used in testing: a gram negative bacterium, a gram positive bacterium, a yeast, and a mold. Testing will determine the presence of diffusible antimicrobial agents, indicative of leaching of the chitosan silver finish, and further testing will determine contact inhibition of the treated textiles. Results of Specific Aim 2 will determine the primary mode of antimicrobial activity, which is anticipated to be via contact inhibition rather than leaching of the chitosan silver finish. Specific Aim 3 will demonstrate the durability of the textile finish during washing and disinfection. Textiles will be washed, with and without bleach, using typical cleaning conditions for healthcare textiles. Antimicrobial efficacy will be tested after 10, 50, and 100 washes using the same methods as described for Specific Aim 2. After each washing stage, silver remaining on the textile will be determined via nitric acid digestion and ICP-MS. Results of Specific Aim 3 will demonstrate the durability of the textile finish during typical cleaning procedures, and verify the retention of the chitosan silver finish. Specific Aim 4 is to determine how the chitosan silver textile finish alters textile properties, specifically, color, water repellency, and flammability. The treated textiles are anticipated to demonstrate minimal graying, no impact on water repellency, and reduced flammability. To demonstrate the utility of this chitosan silver finish for preparing antimicrobial textiles, the anticipated outcome is at least a 3-log reduction for all pathogens for all treated textiles, both unwashed and washed up to 50 times.

项目总结 最多30行文本 最小1/2英寸页边距 字体大小为11磅或更大 所提出的工作的总体研究目标是测试壳聚糖支持的抗菌活性和耐用性 银基纺织饰面，用于医院隐私窗帘。此外，这一点的成功开发 用于隐私窗帘的纺织品整理将为开发额外的抗菌纺织品奠定基础 例如，在床单、毯子、病人衣服、手术服和外科床单中使用。抗菌纺织品提供服务 抗击医院获得性感染，每年影响约200万名患者，并每年直接导致 美国至少284亿美元的医疗成本。为了支持总体研究目标，特定目标1将确定 壳聚糖银产品在与医疗保健领域相关的各种纺织品上的负载率 公差为10%。目前的壳聚糖银产品(1%壳聚糖和0.15%银)的制造将具有公差 10%(0.9-1.1%壳聚糖和0.14-0.16%银)，用于整理棉、涤纶、氨纶和纺织混纺织物， 随后测定了壳聚糖银织物整理剂的负载率。具体目标1的结果将证明 抗菌活性与处理过的纺织品上的壳聚糖和银浓度相关的基础。特定目标2将 测定针对特定目标制备的纺织品的抗菌活性1.在测试中将使用四种微生物： 革兰氏阴性菌、革兰氏阳性菌、酵母菌和霉菌。检测将确定是否存在可扩散的 抗菌剂，指示壳聚糖银表面的淋洗，以及进一步的测试将确定接触抑制 处理过的纺织品。特定目标2的结果将确定抗微生物活性的主要模式，即 预计将通过接触抑制而不是壳聚糖银表面的淋洗来实现。《特定目标3》将演示 纺织品整理在洗涤和消毒过程中的耐用性。用漂白剂和不用漂白剂洗涤纺织品 保健用纺织品的典型清洁条件。在洗涤10次、50次和100次后，使用 与为特定目的描述的方法相同2.在每个洗涤阶段后，残留在纺织品上的银将 采用硝酸消解和电感耦合等离子体质谱联用技术测定。特定目标3的结果将证明纺织品的耐用性 在典型的清洁程序中，请检查表面处理情况，并验证壳聚糖银表面处理的固位力。具体目标4是确定 壳聚糖银纺织品整理剂如何改变纺织品的性能，特别是颜色、憎水性和易燃性。这个 预计经过处理的纺织品将表现出最小的灰化，对防水没有影响，并降低易燃性。 为了证明这种壳聚糖银整理在抗菌纺织品中的应用，预期的结果是 对所有经过处理的纺织品的所有病原体至少减少3个对数，包括未经洗涤和最多50次洗涤。