A new approach to quantitative, point-of-care serology

定量、护理点血清学的新方法

基本信息

批准号：
9306748
负责人：
Kevin W Plaxco
金额：
$ 35.8万
依托单位：
UNIVERSITY OF CALIFORNIA SANTA BARBARA
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2019-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9306748
关键词：
Affinity Antibodies Antibody Repertoire Antigens Appearance Bedside Testings Binding Biological Assay Biological Markers Blood Blood specimen Clinic Clinical Clinical Sensitivity Communicable Diseases Complex DNA Detection Development Devices Diagnosis Diagnostic Disease Effectiveness Electrodes Electronics Elements Epitopes Fingers Fluorescence Polarization Generations Goals Gold HIV HIV Antibodies HIV Seropositivity HIV-1 Hand Health Personnel Herpes Simplex Infections Home environment Human Immune response Infection Laboratories Lateral Length Life Measurable Measurement Measures Medical Methods Military Personnel Modification Molecular Weight Monitor Nucleic Acid Probes Outcome Oxidation-Reduction Patients Peptides Performance Physicians Predictive Value Prevalence Proteins Public Health Reagent Reporter Reporting Reproducibility Research Sampling Sensitivity and Specificity Serodiagnoses Serologic tests Serological Serum Sexually Transmitted Diseases Side Signal Transduction Speed Syphilis Technology Temperature Testing Validation Venous Whole Blood Work World Health Organization analog base clinically actionable clinically relevant cost cost effective design diagnostic biomarker diagnostic panel glucose monitor improved molecular diagnostics multiplex detection novel strategies point of care portability pre-clinical programs protein biomarkers public health relevance research clinical testing response sensor standard of care success

项目摘要

DESCRIPTION (provided by applicant): We have recently demonstrated a reagentless, electrochemical method - termed E-DNA sensors - that achieves the detection of anti-HIV antibodies directly in undiluted, unprocessed human blood serum at concentrations orders of magnitude lower than those seen in HIV-positive patient samples. The approach is rapid (sub-10 min), single-step, and quantitative, thus improving on the convenience and/or clinical value of existing point-of-care molecular diagnostics. Further speaking to its potential value, the approach is supported on micron-scale electrodes and can thus be multiplexed to the level of measuring dozens of diagnostic antibodies in a single finger-prick sample. Given these attributes, our technology appears well suited for applications that would derive value from the ability to measure quantitatively the levels of multiple antibodies outside of central laboratories The focus of the proposed research program is to test this hypothesis by performing the initial, pre-clinical validation of the E-DNA antibody detection platform. Specifically, we will fabricate E DNA arrays for the measurement of one to two dozen antibodies diagnostic of a panel of three sexually transmitted infections, and perform side-by-side comparison of these against current gold standard laboratory approaches when both are challenged using authentic human samples.

描述（由申请人提供）：我们最近证明了一种无试剂，电化学方法 - 称为E-DNA传感器 - 在未稀释的未稀释的，未经处理的人血清中直接在HIV阳性患者样品中观察到的抗HIV抗体的检测直接在未稀释的未经处理的人类血清中检测。该方法是快速（低于10分钟），单步和定量性的，因此可以提高现有护理分子诊断的便利性和/或临床价值。进一步谈到了其潜在值，该方法在微米级电极上得到了支持，因此可以将其多重到单个手指式式样品中测量数十种诊断抗体的水平。鉴于这些属性，我们的技术似乎非常适合应用程序，这些应用可以从定量测量中央实验室以外的多种抗体水平的能力中获得价值。拟议的研究计划的重点是通过对EE-DNA抗体检测平台进行初始的，临床前验证来检验该假设。具体来说，我们将捏造E DNA阵列用于测量三种性传播感染的面板的一到二十抗抗体，并在使用正宗人类样品挑战时与当前的金标准实验室方法进行并排比较。