Development of a Low-Cost Therapy for Biological Toxins for Rural India

为印度农村地区开发低成本生物毒素疗法

• 批准号: 9208599
• 负责人: Claire Komives
• 金额: $ 10.35万
• 依托单位: SAN JOSE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-07 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9208599
• 关键词: Address; Air; Amino Acids; Antidotes; Antitoxins; Antivenins; Biological Response Modifier Therapy; Blood Circulation; C-terminal; Cell Extracts; Chemicals; Chromatography; Cleaved cell; Collaborations; Country; Crotalus; Development; Devices; Didelphidae; Dose; Environment; Equipment; Escherichia coli; Filtration; Formulation; Goals; Health Services Accessibility; Hemorrhage; Immobilization; Immune; In Situ; Inclusion Bodies; India; Industry; Inhalators; Institutes; Lead; Life; Membrane; Metalloproteases; Methods; Modeling; Modification; Morbidity - disease rate; Mus; Names; North American Opossum; Peptide Hydrolases; Peptides; Plasmids; Polymers; Powder dose form; Procedures; Process; Production; Proteins; Research; Rural; Saline; Series; Serum; Snake Bites; Snake Venoms; Snakes; System; Technology; Temperature; Testing; Texas; Therapeutic Intervention; Time; Toxin; Trypsin; Ultrafiltration; Universities; Venoms; anthrax lethal factor; cost; dosage; experimental study; farmer; inhibitor/antagonist; liquid chromatography mass spectrometry; mortality; multidisciplinary; potency testing; process optimization; rural setting; stability testing

Project Summary This project involves the development of a peptide formulation that could be used as a low cost therapy for hemorrhagic snake envenomation. The loss of life due to snake bite is higher in India than in any other country. Indeed, 60% of India's voters are farmers, working in fields where venomous snakes are most prevalent. The expression of a peptide that we have confirmed to neutralize hemotoxic snake venom will be carried out using a bacterial expression system. The peptide can be purified and tested for antivenom activities at the Texas A&M National Natural Toxins Research Center. The peptide will be characterized by LC/MS. Formulations of the peptide will be sought that have long term stability at room temperature and could be appropriate for delivery as a dry powder inhalable formulation or solubilized for alternative delivery method. The mutual expertise of Komives (SJSU) and Rathore (IIT Delhi) will enable the completion of this multidisciplinary project. Specifically, the expression and production of the peptide will be lead by Komives while the purification and process optimization will be lead by Rathore. Formulation will be carried out in both labs, but primarily in the lab of Komives. The project is anticipated to establish an ongoing collaboration between San Jose State University and the India Institute of Technology Delhi.

项目摘要 该项目涉及开发一种可作为低成本使用的多肽配方 出血性蛇毒的治疗。被蛇咬伤造成的生命损失在 印度比其他任何国家都多。事实上，印度60%的选民是农民，他们在田里干活 毒蛇最盛行的地方。我们拥有的一种多肽的表达 确认中和血毒性蛇毒将使用细菌表达 系统。这种多肽可以在德克萨斯A&M进行提纯和抗蛇毒活性测试。 国家天然毒素研究中心。用LC/MS对该多肽进行了表征。 将寻求在室温下具有长期稳定性的多肽配方和 可能适合作为干粉吸入制剂或溶解于 另一种交付方式。 Komives(SJSU)和Rathore(IIT德里)的共同专业知识将使完成 这个多学科的项目。具体地说，多肽的表达和生产将是 Komives将领导提纯和工艺优化，Rathore将领导提纯和工艺优化。 配方将在两个实验室进行，但主要在Komives的实验室进行。该项目是 预计将在圣何塞州立大学和 德里印度理工学院。