Developing Heparan Sulfate Glycan Array

开发硫酸乙酰肝素聚糖阵列

• 批准号: 9408339
• 负责人: JIAN LIU
• 金额: $ 14.99万
• 依托单位: GLYCAN THERAPEUTICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9408339
• 关键词: Antibodies; Bacterial Infections; Binding; Biological; Biological Assay; Biological Process; Blood coagulation; Budgets; Carbohydrates; Cells; Communities; Custom; Embryonic Development; Ensure; Equipment; Extracellular Matrix; Heparitin Sulfate; Human; Immobilization; Inflammatory Response; Label; Letters; Libraries; Licensing; Methods; Microarray Analysis; Monitor; North Carolina; Oligosaccharides; Physiological; Physiology; Play; Polysaccharides; Positioning Attribute; Preparation; Price; Process; Proteins; Reader; Research; Research Support; Role; Sampling; Services; Ships; Slide; Small Business Technology Transfer Research; Source; Structure; Structure-Activity Relationship; Surface; System; Techniques; Technology; Testing; Therapeutic; Tissues; United States National Institutes of Health; Universities; Unspecified or Sulfate Ion Sulfates; Validation; Viral; base; cost; cost effective; design; high throughput screening; improved; innovation; interest; milligram; new technology; phase 1 study; phase 2 study; response; screening; success; tool

Project title: Developing heparan sulfate glycan array This proposal is aimed at developing a high throughput method to study the interactions between heparan sulfate (HS) and proteins. HS is a highly sulfated polysaccharide displaying a wide range of biological functions. Glycan Therapeutics is a company specialized in HS related research products. In this proposal, an HS array technique will be constructed using both a microtiter plate format and a microarray format. The strength of the company is the capability of synthesizing structurally defined HS oligosaccharides using the chemoenzymatic method, allowing the synthesis of HS oligosaccharide in gram scale. This synthetic capability will ensure access to the oligosaccharide substrates at low cost and allows Glycan Therapeutics to have full control over the purity of the substrates. This unique strength will enable Glycan Therapeutics to produce high quality HS microtiter plates and HS micro-array chips at an affordable price. Aim 1 is designed to prepare HS microtiter plates based HS array. The major advantage of HS microtiter plate is that they are reusable and convenient. No additional equipment other than a microplate reader is needed. Furthermore, this method can be expanded to investigate the roles of HS in cell-based functional assays. Aim 2 is to prepare HS microarray technique. This technique will allow screening a very large number of HS oligosaccharides and proteins using very small amounts of carbohydrate and protein samples. The HS microarray technology will be readily integrated with existing carbohydrate-array methods in a centralized facility specialized in carbohydrate screening. In phase I studies, a library of 60 HS oligosaccharides will be affixed on a microtiter plate or on a microarray slide for biological screening. The HS microtiter plates or array slides will be tested thoroughly to ensure quality. In the phase II studies, the number of oligosaccharides on the microtiter plates and array slides will be expanded to 200 to fully represent the structures of HS isolated from natural sources. Success will lead to a convenient and reliable approach for improving research in glycosciences.

项目名称：开发硫酸乙酰肝素多聚糖阵列 这项建议旨在开发一种高通量方法来研究肝素之间的相互作用。 硫酸盐(HS)和蛋白质。HS是一种高度硫酸盐化的多糖，具有广泛的生物学活性 功能。葡聚糖治疗是一家专门从事HS相关研究产品的公司。在这项提案中， HS阵列技术将使用微滴定板格式和微阵列格式构建。这个 该公司的优势是有能力利用 化学酶法，可合成克级的HS寡糖。这种综合能力 将确保以低成本获得低聚糖底物，并允许葡聚糖治疗药物充分 控制底物的纯度。这种独特的力量将使葡聚糖治疗公司能够产生高 质优价廉的HS微滴定板和HS微阵列芯片。AIM 1旨在为HS做准备 基于微滴定板的HS阵列。HS微滴定板的主要优点是它们可重复使用和 很方便。除了微孔板阅读器外，不需要额外的设备。此外，该方法可以 以研究HS在基于细胞的功能分析中的作用。目标2：制备HS微阵列 技术。这项技术将允许筛选非常大量的HS寡糖和蛋白质 非常少量的碳水化合物和蛋白质样本。HS微阵列技术将很容易 与现有的碳水化合物阵列方法集成在专门研究碳水化合物的集中式设施中 放映。 在第一阶段的研究中，60个HS寡糖的文库将被固定在微滴定板或微阵列上 用于生物筛选的载玻片。HS微量板或阵列载玻片将进行彻底测试，以确保 质量。在第二阶段的研究中，微量平板和阵列载玻片上的寡糖数量将为 扩展到200以完全表示从自然来源分离的HS的结构。成功将带来一个 改进糖科学研究的方便和可靠的方法。