Orbitrap Fusion Lumos ETD

摘要 糖蛋白分析是蛋白质质谱学的一个专门领域。肽的行为不同 基本上来自糖基化肽的行为，糖基化肽的分析需要调整分析方法。 很少有实验室提供糖蛋白组学和糖蛋白表征服务，因为需要 用于调整分析技术和数据解释工具。戈德曼博士的实验室 在分析糖蛋白质组学方面的专业知识，并作为糖蛋白质组学的主要中心， 华盛顿特区。在这里，我们请求资金用于Orbitrap Fusion Lumos ETD质谱仪， 我们的糖科学研究计划支持的糖蛋白组学项目。该混合质量分析仪具有 功能的特殊组合，有助于糖蛋白质组学分析。新优化的ETD 裂解增强了糖肽的CID/HCD裂解，离子阱的MSn能力 能够实现糖肽和聚糖片段的结构解析，以及前体和 碎片离子允许糖肽的可靠鉴定。NCRR的支持将使我们能够 为乔治敦大学和合作机构的资助项目提供专家支持;这种支持 目前在华盛顿地区甚至在国家一级都没有。支持的必要性 糖科学最近得到了美国国家科学院的认可，该提案旨在 缩小生物医学研究界糖蛋白组学解决方案可用性的差距。

ABSTRACT Analysis of glycoproteins is a specialized field of protein mass spectrometry. Behavior of peptides differs substantially from the behavior of glycosylated peptides whose analysis requires adjusted analytical methods. Few laboratories provide services in glycoproteomics and glycoprotein characterization because of the need for adjustments of the analytical technics and data interpretation tools. The laboratory of Dr. Goldman has documented expertize in analytical glycoproteomics and serves as a major hub for glycoproteomics in the Washington DC area. Here we request funds for an Orbitrap Fusion Lumos ETD mass spectrometer to enable glycoproteomic projects supported by our glycoscience research program. This hybrid mass analyzer has an exceptional combination of functionalities that facilitates glycoproteomic analyses. The newly optimized ETD fragmentation enhances the CID/HCD fragmentation of glycopeptides, the MSn capabilities of the ion trap enable structural resolution of the glycopeptide and glycan fragments, and the high resolution of precursor and fragment ions allows confident identification of the glycopeptides. The support from NCRR will enable us to provide expert support to funded projects at Georgetown University and collaborating institutions; such support is currently not available in the Washington DC area or even at the National level. The need for support of glycoscience was recently recognized by the National Academy of Sciences and this proposal intends to narrow the gap in the availability of glycoproteomic solutions for the biomedical research community.