timsTOF Pro Mass Spectrometer

摘要 糖蛋白分析是蛋白质质谱学的一个专门领域。肽的行为不同 基本上来自糖基化肽的行为，糖基化肽的分析需要调整分析方法。 很少有实验室提供糖蛋白组学和糖蛋白表征服务，因为需要 用于调整分析技术和数据解释工具。临床研究人员和 乔治城大学的转化糖科学研究中心（CTGRC）拥有记录在案的专业知识 CTGRC是华盛顿糖蛋白组学的主要中心 DC地区在这里，我们申请资金用于timsTOF Pro质谱仪，以实现糖蛋白组学项目 由CTGRC支持。该质谱仪具有捕获离子迁移率与 高速、高分辨率QTOF质谱分析仪。新优化的工作流程可实现高效分离， 在timsTOF Pro上的糖肽片段允许在检测、分辨率、 和糖肽的定量。NCRR的支持将使我们能够提供专家支持， 在高需求和短缺，资助项目在乔治敦大学，在华盛顿地区， 在国家一级。

ABSTRACT Analysis of glycoproteins is a specialized field of protein mass spectrometry. Behavior of peptides differs substantially from the behavior of glycosylated peptides whose analysis requires adjusted analytical methods. Few laboratories provide services in glycoproteomics and glycoprotein characterization because of the need for adjustments of the analytical technics and data interpretation tools. Researchers at the Clinical and Translational Glycoscience Research Center (CTGRC) at Georgetown University have documented expertise in analytical glycoproteomics and the CTGRC serves as a major hub for glycoproteomics in the Washington DC area. Here we request funds for a timsTOF Pro mass spectrometer to enable glycoproteomic projects supported by the CTGRC. This mass spectrometer has an exceptional combination of trapped ion mobility with high speed and resolution QTOF mass analyzer. The newly optimized workflows for efficient separation and fragmentation of glycopeptides on the timsTOF Pro allow unprecedented sensitivity in the detection, resolution and quantification of glycopeptides. The support from NCRR will enable us to provide expert support, currently in high demand and short supply, to funded projects at Georgetown University, in the Washington DC area, and at the National level.