timsTOF Pro Mass Spectrometer

timsTOF Pro 质谱仪

• 批准号: 10173053
• 负责人: RADOSLAV GOLDMAN
• 金额: $ 60万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10173053
• 关键词: Area; Behavior; Clinical; Data Analyses; Detection; District of Columbia; Funding; Glycopeptides; Glycoproteins; Laboratories; Mass Spectrum Analysis; National Center for Research Resources; Peptides; Proteins; Research; Research Personnel; Resolution; Services; Speed; Universities; analytical method; glycoproteomics; ion mobility; mass analyzer; mass spectrometer; tool

ABSTRACT Analysis of glycoproteins is a specialized field of protein mass spectrometry. Behavior of peptides differs substantially from the behavior of glycosylated peptides whose analysis requires adjusted analytical methods. Few laboratories provide services in glycoproteomics and glycoprotein characterization because of the need for adjustments of the analytical technics and data interpretation tools. Researchers at the Clinical and Translational Glycoscience Research Center (CTGRC) at Georgetown University have documented expertise in analytical glycoproteomics and the CTGRC serves as a major hub for glycoproteomics in the Washington DC area. Here we request funds for a timsTOF Pro mass spectrometer to enable glycoproteomic projects supported by the CTGRC. This mass spectrometer has an exceptional combination of trapped ion mobility with high speed and resolution QTOF mass analyzer. The newly optimized workflows for efficient separation and fragmentation of glycopeptides on the timsTOF Pro allow unprecedented sensitivity in the detection, resolution and quantification of glycopeptides. The support from NCRR will enable us to provide expert support, currently in high demand and short supply, to funded projects at Georgetown University, in the Washington DC area, and at the National level.

抽象的 糖蛋白分析是蛋白质质谱分析的一个专业领域。肽的行为不同 基本上来自糖基化肽的行为，其分析需要调整分析方法。 由于需要，很少有实验室提供糖蛋白组学和糖蛋白表征服务 用于调整分析技术和数据解释工具。临床和 乔治城大学转化糖科学研究中心 (CTGRC) 拥有记录的专业知识 CTGRC 是华盛顿糖蛋白质组学的主要中心 直流地区。在此，我们请求资金购买 timsTOF Pro 质谱仪，以支持糖蛋白组项目 得到CTGRC的支持。该质谱仪将俘获离子淌度与 高速、高分辨率 QTOF 质量分析仪。新优化的工作流程可实现高效分离和 timsTOF Pro 上的糖肽碎片可实现前所未有的检测灵敏度、分辨率 和糖肽的定量。 NCRR 的支持将使我们能够提供专家支持，目前 华盛顿特区乔治敦大学的资助项目需求量大但供应不足， 以及国家层面。