timsTOF Pro Mass Spectrometer

摘要 糖蛋白分析是蛋白质质谱学的一个专门领域。多肽的行为不同 基本上是由于糖基化多肽的行为，其分析需要调整分析方法。 由于需要，很少有实验室提供糖蛋白组学和糖蛋白鉴定方面的服务 用于分析技术和数据解释工具的调整。临床和临床研究人员 乔治敦大学的翻译糖科学研究中心(CTGRC)已经记录了专业知识 在分析糖蛋白组学方面，CTGRC是华盛顿糖蛋白组学的主要枢纽 华盛顿特区。在这里，我们申请了一台TimsTOF Pro质谱仪的资金，以支持糖蛋白质组项目 由CTGRC支持。该质谱计具有捕获离子迁移率和 高速、高分辨率的QTOF质量分析仪。新优化的工作流可实现高效分离和 TimsTOF Pro上糖肽的碎裂使检测、分辨率 和糖肽的定量。NCRR的支持将使我们能够提供专家支持，目前 在供不应求的情况下，为乔治城大学华盛顿特区地区的项目提供资金， 在国家层面上。

ABSTRACT Analysis of glycoproteins is a specialized field of protein mass spectrometry. Behavior of peptides differs substantially from the behavior of glycosylated peptides whose analysis requires adjusted analytical methods. Few laboratories provide services in glycoproteomics and glycoprotein characterization because of the need for adjustments of the analytical technics and data interpretation tools. Researchers at the Clinical and Translational Glycoscience Research Center (CTGRC) at Georgetown University have documented expertise in analytical glycoproteomics and the CTGRC serves as a major hub for glycoproteomics in the Washington DC area. Here we request funds for a timsTOF Pro mass spectrometer to enable glycoproteomic projects supported by the CTGRC. This mass spectrometer has an exceptional combination of trapped ion mobility with high speed and resolution QTOF mass analyzer. The newly optimized workflows for efficient separation and fragmentation of glycopeptides on the timsTOF Pro allow unprecedented sensitivity in the detection, resolution and quantification of glycopeptides. The support from NCRR will enable us to provide expert support, currently in high demand and short supply, to funded projects at Georgetown University, in the Washington DC area, and at the National level.