5600 TripleTOF Mass Spectrometry

描述（由申请人提供）：5600 TripleTOF是AB Sciex的新系列质谱仪，据我们所知，在大华盛顿地区的任何实验室都无法使用。根据文件需求和全面评估，本提案要求为购买5600 TripleTOF质谱仪（AB Sciex）提供资金。5600 TripleTOF将支持华盛顿特区乔治城大学医学中心的质谱医学应用（NIH， DOD， NSF等），包括NCI指定的综合癌症中心伦巴第癌症中心。乔治城大学慷慨地支持蛋白质组学研究，并承诺长期致力于质谱资源的维护和运行。NCRR的支持对于帮助我们在当前的经济环境下升级仪器将是无价的，并将使我们有机会完成使用乔治敦大学现有仪器无法有效开展的项目。质谱服务集中在蛋白质组学和代谢组学共享资源（PMSR）中，该资源的任务是将可用的技术提供给乔治城大学医学中心的广泛研究社区。蛋白质组学服务由Goldman博士指导，涵盖三个主要研究领域：蛋白质组的2D-LC-MS/MS表征；定量蛋白质组学比较；在蛋白质糖基化分析中具有特殊服务的蛋白质修饰的表征。5600的性能大大改善了所有这些研究领域，也许最显著的是复杂样品的定量比较。蛋白糖型分析是我们的专业服务，用户需求快速增长；5600 TripleTOF将极大地帮助我们在这个总体上没有充分覆盖的研究领域开发服务。在本应用程序中，我们描述了几个已经获得资助的令人兴奋的研究项目，这些项目将通过升级我们的仪器到5600 TripleTOF而成为可能。

DESCRIPTION (provided by applicant): The 5600 TripleTOF is a new line of mass spectrometer from AB Sciex that is not yet available to our knowledge in any laboratory in the greater Washington DC area. This proposal requests funds for the purchase of a 5600 TripleTOF mass spectrometer (AB Sciex) based on documented need and thorough evaluation. The 5600 TripleTOF will support funded biomedical applications of mass spectrometry (NIH, DOD, NSF etc) at Georgetown University Medical Center, Washington DC including Lombardi Cancer Center, an NCI designated Comprehensive Cancer Center. Georgetown University has generously supported proteomic research, and promises long term commitment to the maintenance and operation of the mass spectrometric resources. The support from NCRR will be invaluable in helping us upgrade the instrumentation in the current economic climate, and will give us the opportunity to complete projects that cannot be efficiently carried out using the currently available instrumentation at Georgetown University. The mass spectrometric services are centralized in the Proteomic and Metabolomic Shared Resource (PMSR) which has the mandate to make the available technology available to a broad research community at the Georgetown University Medical Center. Proteomic services, directed by Dr. Goldman, cover three major areas of research: 2D-LC-MS/MS characterization of proteomes; quantitative proteomic comparisons; and characterization of protein modifications with specialty services in the analysis of protein glycosylation. The performance of the 5600 greatly improves all these areas of research and perhaps most dramatically the quantitative comparisons of complex samples. Analysis of protein glycoforms is our specialty service with rapidly growing user demand; the 5600 TripleTOF will help us significantly in the development of services in this, in general, insufficiently covered area of research. In this application, we describe several already-funded exciting research projects that will be made possible by the upgrade of our instrumentation to the 5600 TripleTOF.