5600 TripleTOF Mass Spectrometry

描述(由申请人提供)：5600 TripleTOF是AB Sciex的一种新的质谱仪系列，据我们所知，在大华盛顿特区地区的任何实验室都无法获得。这项提案要求提供资金，用于购买一台5600 TripleTOF质谱仪(AB Sciex)，这是基于书面需求和彻底评估的结果。5600 TripleTOF将支持华盛顿特区乔治敦大学医学中心(包括NCI指定的综合癌症中心Lombardi癌症中心)资助的质谱学生物医学应用(NIH、DOD、NSF等)。乔治城大学慷慨支持蛋白质组研究，并承诺长期致力于质谱学资源的维护和操作。NCRR的支持将有助于我们在当前的经济环境下升级仪器，并将使我们有机会完成使用乔治敦大学目前可用的仪器无法有效执行的项目。质谱学服务集中在蛋白质和新陈代谢共享资源(PMSR)中，该资源的任务是将可用的技术提供给乔治敦大学医学中心的广泛研究社区。由Goldman博士指导的蛋白质组学服务涵盖三个主要研究领域：蛋白质组的2D-LC-MS/MS表征；定量蛋白质组学比较；以及在蛋白质糖基化分析中提供专门服务的蛋白质修饰的表征。5600的性能极大地改进了所有这些研究领域，也许最显著的是对复杂样品的定量比较。蛋白质糖型分析是我们的专业服务，用户需求迅速增长；5600 TripleTOF将极大地帮助我们开发这一总体上覆盖不足的研究领域的服务。在这份申请中，我们描述了几个已经资助的令人兴奋的研究项目，这些项目将通过我们的仪器升级到5600 TripleTOF而成为可能。

DESCRIPTION (provided by applicant): The 5600 TripleTOF is a new line of mass spectrometer from AB Sciex that is not yet available to our knowledge in any laboratory in the greater Washington DC area. This proposal requests funds for the purchase of a 5600 TripleTOF mass spectrometer (AB Sciex) based on documented need and thorough evaluation. The 5600 TripleTOF will support funded biomedical applications of mass spectrometry (NIH, DOD, NSF etc) at Georgetown University Medical Center, Washington DC including Lombardi Cancer Center, an NCI designated Comprehensive Cancer Center. Georgetown University has generously supported proteomic research, and promises long term commitment to the maintenance and operation of the mass spectrometric resources. The support from NCRR will be invaluable in helping us upgrade the instrumentation in the current economic climate, and will give us the opportunity to complete projects that cannot be efficiently carried out using the currently available instrumentation at Georgetown University. The mass spectrometric services are centralized in the Proteomic and Metabolomic Shared Resource (PMSR) which has the mandate to make the available technology available to a broad research community at the Georgetown University Medical Center. Proteomic services, directed by Dr. Goldman, cover three major areas of research: 2D-LC-MS/MS characterization of proteomes; quantitative proteomic comparisons; and characterization of protein modifications with specialty services in the analysis of protein glycosylation. The performance of the 5600 greatly improves all these areas of research and perhaps most dramatically the quantitative comparisons of complex samples. Analysis of protein glycoforms is our specialty service with rapidly growing user demand; the 5600 TripleTOF will help us significantly in the development of services in this, in general, insufficiently covered area of research. In this application, we describe several already-funded exciting research projects that will be made possible by the upgrade of our instrumentation to the 5600 TripleTOF.