Mouse Genetic Models for Alcohol Excessive Drinking and Dependence

 描述（由申请人提供）：我们保留了大量独特的基因型小鼠用于酒精研究。这些动物用于多个R 01、U 01和多个VA Merit Review赠款支持的研究。这些动物已经被波特兰、俄勒冈州和世界各地许多其他地点的研究人员用于研究。对这些基因型的部分支持来自波特兰酒精研究中心（PARC）P60补助金，Crabbe博士的VA Merit Review补助金，并已在雇用它们的主要赠款内预算。成本膨胀以及拨款周期和实际使用之间缺乏同步性，导致我们在支持这些小鼠的能力方面越来越落后。我们无法跟上的部分原因是通货膨胀超过了NIH COLA的限制，部分原因是几个主要支持赠款（P60，U 01和VA Merit Reviews）的预算上限。此外，PARC P60补助金在2016年1月1日更新时将不再使用这些基因型，因此这种费用分摊来源将失去。拟议的R24寻求适度的支持，以维持多种基因型，在每种情况下，与使用费和父母赠款的维持支持分摊费用。有四个目标：目标1。帮助维持长期选择品系及其遗传异质对照品系（WSP-1、WSP-2、WSR-1、WSR-2、WSC、HDID-1、HDID-2和HS/Npt）的核心育种菌落。目标二。帮助保持核心育种群体的同类株系和品系以及转基因或敲除株系，用于数量性状位点（QTL）基因定位研究。目标3.部分支付长期选择品系、同源品系和转基因品系的冷冻保存费用。冷冻保存防止关键基因型的灾难性损失，以及为潜在的未来使用提供途径[选择的品系WSP-1、WSP-2、WSR-1、WSR-2、HDID-1、HDID-2和敲除B6.GIRK3-/-]。目标4。部分支付维持乙醇蒸汽吸入核心产生身体依赖的费用。这个核心支持多个赠款和调查人员。PARC P60赠款的费用分摊将不再提供，因为PARC在更新中的研究将不使用身体依赖。所有涉及的基因型都提供给感兴趣的研究人员。不需要资金来支持分发，因为这种支持是在支持各种基因型的基本赠款下提供的。这里也不要求提供资金来支持额外动物的繁殖和测试，而是在父母赠款下提供。

 DESCRIPTION (provided by applicant): We maintain a large number of unique genotypes of mice for alcohol research studies. These animals are used in research supported by multiple R01s, U01s, and several VA Merit Review Grants. These animals have been used for research by investigators in Portland, Oregon and at many other sites around the world. Partial support for these genotypes has come from the Portland Alcohol Research Center (PARC) P60 grant, Dr. Crabbe's VA Merit Review grant, and has been budgeted within the primary grants employing them. Cost inflation and the lack of synchrony between grant cycles and actual use has led us to fall further and further behind in our ability to support these mice. Our inability t keep up is due partly to inflation exceeding the NIH COLA limits, and partly to budget caps on several of the principal supporting grants (P60, U01s, and VA Merit Reviews). Furthermore, the PARC P60 grant will no longer be using these genotypes when it renews Jan 1 2016, so this source of cost-sharing will be lost. The proposed R24 seeks modest support to maintain multiple genotypes, in each case cost-shared with user fees and maintenance support from the parent grants. There are four aims: Aim 1. Help maintain core breeding colonies of long-term selected lines and their genetically heterogeneous control lines (WSP-1, WSP-2, WSR-1, WSR-2, WSC, HDID-1, HDID-2, and HS/Npt). Aim 2. Help maintain core breeding colonies of congenic strains and lines and transgenic or knockout strains, for quantitative trait locus (QTL) gene mapping studies. Aim 3. Partially defray costs for cryopreservation of long term selected lines and congenic and transgenic strains. Cryopreservation protects against catastrophic loss of crucial genotypes as well as providing access for potential future use [selected lines WSP-1, WSP-2, WSR-1, WSR-2, HDID-1, HDID-2, and the knockout B6.GIRK3-/-]. Aim 4. Partially defray costs of maintaining an ethanol vapor inhalation core for producing physical dependence. This core supports multiple grants and investigators. Cost-sharing from the PARC P60 grant will no longer be available as PARC studies in the renewal will not employ physical dependence. All genotypes covered are made available to interested investigators. Funds to support distribution are not requested, as this support is provided under the primary grants supporting the various genotypes. Funds to support breeding and testing of additional animals are also not requested here, but rather under the parent grants.