Animal Core

甲基苯丙胺滥用研究中心（MARC）动物核心将通过创建MA饮用的选定品系，维护这种遗传小鼠模型，协助中心所需的行为测试标准化，优先分配和分配小鼠，从商业供应商购买小鼠，以及维护与MARC生物统计学和遗传学核心维护的实验结果数据库结合使用的动物跟踪数据库。动物核心将由John Crabbe博士管理，他在开发成瘾相关性状的遗传动物模型方面经验丰富，并将根据需要监督MARC所有科学组成部分和新飞行员的小鼠维护和提供。动物核心产生的资源也将提供给外部研究者。Crabbe博士将监督一个经验丰富的工作人员;该工作人员已经合作或在Crabbe博士的实验室工作多年。所有动物将在有执照兽医的全时监督下饲养在AALAC认证的动物设施中。已制定了避免病毒和细菌污染的程序，并使用哨兵筛查程序快速识别污染并避免传播。为保持大量基因型而进行的仔细的遗传记录和鉴定做法已经实行了好几年。Animal Core的主要优势是与集中开发相关的成本节约;所选品系小鼠的行为测试和分布;组件8、9和10的行为测试标准化，而不是扩展到其他用户;受控的环境条件;遗传质量控制;从组件PI中去除动物生产负担。关于动物分配的决定将集中在一系列标准的基础上，包括申请日期，申请是否是MARC项目，科学进展以及最后一次分配给该项目的日期。核心将能够在项目之间分配同窝仔，以最大限度地减少研究中的窝仔效应，并增加跨实验关联分析的可能性。核心做法将加强机读目录产生的数据。

The Methamphetamine Abuse Research Center (MARC) Animal Core will serve the Center by creating selected lines for MA drinking, maintaining this genetic mouse model, assisting with the standardization of behavioral tests needed by the Center, prioritizing distribution and distributing mice, purchasing mice from commercial suppliers, and maintaining an animal tracking database to be used in conjunction with experimental results databases maintained by the MARC Biostatistics and Genetics Core. The Animal Core will be managed by Dr. John Crabbe, who is experienced in developing genetic animal models of addiction-related traits and will oversee maintenance and provision of mice for all Scientific Components of the MARC and for new pilots on an as needed basis. Animal Core generated resources will also be made available to outside investigators. Dr. Crabbe will supervise an experienced staff; members of that staff have been working collaboratively or within Dr. Crabbe's lab for many years. All animals will be housed in AALAC accredited animal facilities under the full-time supervision of a licensed veterinarian. Procedures are in place for the avoidance of viral and bacterial contamination, and a sentinel screening program is used to quickly identify contamination and avoid spread. Careful genetic record-keeping and identification practices for the maintenance of a large number of genotypes have been in place for several years. Key advantages of the Animal Core are cost savings associated with centralized development; behavioral testing and distribution of the selected line mice; standardization of behavioral testing across Components 8 and 9 and 10 than can be extended to other users; controlled environmental conditions; genetic quality control; removal of animal production burden from Component PIs. Decisions about animal distribution will be centralized and based on a number of criteria, including date of request, whether the request is for a MARC project, scientific progress, and date of last distribution to that project. The Core will be able to distribute littermates across projects to minimize litter effects within studies, and increase the potential for cross-experiment association analyses. Core practices will strengthen the data generated by the MARC.