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Transcripts and Functions Targeted by Non-coding RNAs in Palate Development

上颚发育中非编码 RNA 靶向的转录本和功能

基本信息

批准号：
9165356
负责人：
Junichi Iwata
金额：
$ 23.1万
依托单位：
UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-01 至 2018-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9165356
关键词：
Accounting Affect Anterior Base Sequence Bioinformatics Biological Cell Count Cell Proliferation Cells Cleft Palate Code Complex DNA Sequence Data Data Analyses Data Set Databases Development Diagnosis Disease Early Diagnosis Embryo Environmental Risk Factor Ethnic Origin Etiology Event Family Future Gender Gene Expression Gene Expression Regulation Gene Mutation Gene Targeting Genes Genetic Genetic Transcription Genetic study Genome Goals Health Hour Human Image In Situ Hybridization Individual Internet Lateral Lead Linear Models Link Maps Medial Mesenchymal Messenger RNA Meta-Analysis MicroRNAs Morphogenesis Mus Organ Palate Pattern Phenotype Play Population Prevalence Prevention Process Proteins Quality Control RNA RNA-Protein Interaction Reading Reverse Transcriptase Polymerase Chain Reaction Role Scanning Electron Microscopy Specificity Staging Structure Technology Testing Therapeutic Tissues Transact Transcript Translating Untranslated RNA Variant base design differential expression genetic approach genetic information genome-wide human genome sequencing insight overexpression palatogenesis prevent public health relevance research study systematic review tool transcriptome transcriptome sequencing

项目摘要

ABSTRACT The cause of cleft palate is influenced by multiple complex genetic and environmental factors. During palate morphogenesis, hundreds of gene expressions dramatically change at each embryonic stage. However, it is still largely unknown how these gene expressions are regulated. Recent studies reveal that non-coding RNAs (ncRNAs: 98% of transcriptome) affect the expression of genes encoded by coding RNAs (aka messenger RNAs, mRNAs) as well as the phenotype without altering the DNA sequence, and contribute to a number of diseases. Although most genetic information is transacted by proteins through mRNAs, the majority of the genome is transcribed into ncRNAs including microRNAs (miRs) and long non-coding RNAs (lncRNAs). We hypothesize that the proper control of ncRNAs, which play important roles in palate development, is crucial for the regulation of genes, due to the fact that their disruption causes cleft palate. To identify ncRNAs involved in palate development, we will analyze 1) FaceBase1 miR sequencing (miR-seq) datasets; 2) FaceBase1 mRNA microarray datasets; and 3) FaceBase1 RNA sequencing (RNA-seq) datasets, from the developing mouse palate. In addition, to predict ncRNAs involved in cleft palate from previous genetic studies in humans and mice, we will perform systematic review and meta-analyses for cleft palate causative genes. The findings by bioinformatics analyses will then be validated experimentally. Our specific aims are to 1) determine the genes and their functions regulated by miRs during palate development; 2) identify lncRNAs involved in palate development; and 3) determine the direct biological link of ncRNAs to the phenotype. This study will provide insight into the role of ncRNAs in palate development and suggest possible strategies for the diagnosis and prevention of cleft palate.

摘要