ShEEP Proposal for a Multiuser Advanced Biosafe Flow Cytometer

摘要 这项请求是为了BD FACSAria Fusion流式细胞仪，它将作为多用户使用 在山上之家医疗中心的核心设施中的仪器。该系统将显著地 加强对主要用户的研究，他们正在进行VA资助的研究和多项 次要用户。虽然这台细胞仪将主要用于VA资助的研究，但仪器时间 还将提供给新的调查人员和寻求获得初步数据的调查人员 用于提交退伍军人管理局补助金。山居退伍军人医疗中心目前没有可靠的 多用户流式细胞仪，目前使用的是陈旧的FacsCalibur流式细胞仪 于2000年购买，并由奎伦医学院拥有和运营；而 参与调查的研究人员继续广泛使用细胞仪。 应用程序，它的颜色选择有限，没有分拣能力，而且必须收费。所有的 弗吉尼亚大学和大学的研究人员依赖于这种流式细胞仪。细胞检测技术 在过去的几年里有了巨大的进步，我们正在研究的问题 目前的要求要求我们使用最先进的细胞学技术，而不是 在我们目前的仪器上可用。很明显，我们目前的细胞仪 不满足退伍军人管理局资助的调查人员的需求，他们中的许多人不仅需要分类 功能齐全，生物安全柜一体化，满足BioProtection的要求 在他们的翻译研究中与生俱来。此外，没有可与之媲美的流式细胞仪 本申请中要求的BD FACSAria Fusion，距离退伍军人管理局方圆200英里。 因此，这一应用解决了一个重大的研究需求。山上之家退伍军人管理局 坚定的机构承诺购买和维护流式细胞仪。一个 500平方英尺英国《金融时报》配备了适当设备以存放乐器的房间已经分配。 已作出规定，在初始保修期过后即可承保服务合同 已经过期了。已经成立了一个咨询委员会。博士级别的科学家，拥有 在流式细胞仪方面有经验，将在有经验的人的协助下监督设施 研究技术员。这一工具将极大地改进Mountain的研究工作 退伍军人之家旨在改善退伍军人的健康状况。

Abstract This request is for a BD FACSAria Fusion flow cytometer which will function as a multiuser instrument in a core facility at the Mountain Home Medical Center. The system will significantly enhance the research of major users who are conducting VA funded research and multiple minor users. While this cytometer will primarily be used for VA funded research, instrument time will also be provided to new investigators and investigators seeking to obtain preliminary data for submission of VA grants. The Mountain Home VA Medical Center currently has no reliable multiuser flow cytometer, and currently utilizing an aged FacsCalibur flow cytometer that was purchased in 2000 and which is owned and operated at the Quillen College of Medicine; while the cytometer continues to be used extensively by the investigators participating in this application, it has limited color options, no sorting capacity, and must be fee-based. All of the VA and University investigators are dependent on this flow cytometer. Cytometric technology has advanced dramatically in the last several years and the research questions that we are currently asking require that we employ state-of-the-art cytometric techniques that are not available on our current instrument. We are clearly at the point where our current cytometer does not meet the needs of VA funded investigators, many of whom require not only sorting capabilities but also an integrated biosafety cabinet to meet the requirements of bioprotection inherent in their translational studies. Furthermore, there are no flow cytometers comparable to the BD FACSAria Fusion, requested in this application, within a 200 mile radius of our VA. Therefore, this application addresses a significant research need. The Mountain Home VA has a strong institutional commitment to the acquisition and maintenance of the flow cytometer. A 500 sq. ft. room, that is appropriately outfitted to house the instrument, has been allocated. Provisions have been made to underwrite the service contract once the initial warranty period has expired. An advisory committee has been established. A doctoral level scientist, with experience in flow cytometry, will supervise the facility with the assistance of an experienced research technician. This instrumental will significantly improve the research efforts at Mountain Home VA aimed at improving veterans' health.