Multiuser Advanced Confocal Microscope

多用户高级共焦显微镜

• 批准号: 9791445
• 负责人: Zhi Q. Yao
• 金额: --
• 依托单位: JAMES H QUILLEN VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2019-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9791445
• 关键词: Address; Advisory Committees; Basic Science; Cell Communication; Cells; Charge; Clinical; Clinical Sciences; Communicable Diseases; Confocal Microscopy; Contract Services; Core Facility; Data Analyses; Data Quality; Fees; Funding; Grant; HIV/HCV; Health; Home environment; Hour; Hybrids; Image; Imaging Techniques; Imaging technology; Immune; Immune System Diseases; Immunologics; Lasers; Maintenance; Manuals; Medical center; Medicine; Microscope; Minor; Noise; Optics; Population; Radial; Research; Research Personnel; Resolution; Sampling; Scanning; Scientist; Signal Transduction; Speed; Supervision; System; Technology; Time; Tissues; Translational Research; Universities; Veterans; base; chronic infection; cohort; college; confocal imaging; cost; data submission; detector; experience; experimental study; improved; in situ imaging; instrument; live cell imaging; programs; quantitative imaging; response; software systems; square foot; tissue culture; vaccine response

Abstract This request is for a Leica SP8 Confocal Microscope which will function as a multiuser instrument in a core facility at the Mountain Home Medical Center. The system will significantly enhance the research of major users who are conducting VA funded research and multiple minor users. While this microscope will primarily be used for VA funded research, instrument time will also be provided to new investigators and investigators seeking to obtain preliminary data for submission of VA grants. The Mountain Home VA Medical Center currently has no confocal microscope, and currently relies upon a fee-based instrument at the adjacent university. While that microscope continues to be used extensively by the investigators participating in this application, the VA researchers are not a priority for instrument use and the charges for its use are steep. Confocal technology has advanced dramatically in the last several years and the research questions that we are currently asking require that we employ state-of- the-art imaging techniques that are not available on our current instrument. We are clearly at the point where our current fee-based instrument does not meet the needs of VA funded investigators, with multiple VA Merit- and DOD-funded studies relying on confocal microscopy. There are no other confocal microscopes for use within a 200 mile radius of our VA. Therefore, this application addresses a significant research need. The Mountain Home VA has a strong institutional commitment to the acquisition and maintenance of the confocal microscope. A 500 sq. ft. room, that is appropriately outfitted to house the instrument, has been allocated. Provisions have been made to underwrite the service contract once the initial warranty period has expired. An advisory committee has been established. A doctoral level scientist, with experience in confocal microscopy, will supervise the facility with the assistance of an experienced research technician. This instrument will significantly improve the research efforts at Mountain Home VA aimed at improving veterans' health.

摘要 这项请求是为了一台徕卡SP8共聚焦显微镜，它将作为多用户使用 在山上之家医疗中心的核心设施中的仪器。该系统将显著地 加强对主要用户的研究，他们正在进行VA资助的研究和多项 次要用户。虽然这台显微镜将主要用于VA资助的研究，但仪器 还将为新的调查人员和寻求获得初步证据的调查人员提供时间 用于提交退伍军人管理局补助金的数据。山居退伍军人医疗中心目前没有 共聚焦显微镜，目前依赖于相邻的收费仪器 上大学。虽然这种显微镜继续被调查人员广泛使用 参与这项申请，退伍军人管理局的研究人员不是仪器使用的优先事项， 使用它的费用很高。在过去的几年中，共焦技术取得了巨大的进步 多年来，我们目前提出的研究问题要求我们使用最新的 最先进的成像技术，在我们目前的仪器上是不可用的。我们显然是在 我们目前的收费工具不能满足退伍军人管理局资助的需求 研究人员，依靠共聚焦显微镜进行了多项由退伍军人事务部和国防部资助的研究。 在退伍军人管理局方圆200英里的范围内，没有其他的共焦显微镜可供使用。因此， 这一应用解决了一个重要的研究需求。山居VA有很强的 机构对购置和维护共焦显微镜的承诺。A 500 平方。英国《金融时报》配备了适当设备以存放乐器的房间已经分配。 已作出规定，在初始保修期过后承保服务合同 已经过期了。已经成立了一个咨询委员会。博士级别的科学家，拥有 具有共焦显微镜经验，将在一名 经验丰富的研究技术员。这一工具将显著改善研究工作。 在退伍军人之家，弗吉尼亚州旨在改善退伍军人的健康。