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Aging and Transgenic Animal Core (Core C)

衰老和转基因动物核心（核心C）

基本信息

批准号：
9147551
负责人：
Fernando Macian
金额：
$ 30.63万
依托单位：
ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
依托单位国家：
美国
项目类别：
财政年份：
2009
资助国家：
美国
起止时间：
2009-02-15 至
项目状态：
未结题

项目摘要

The Aging and Transgenic Animal Core (Core C) will continue to support the need for in vivo experimental mouse models of the four projects of this Program Project. The long-term goals of this Core is to provide the projects with a wide repertoire of age-controlled animal models with modified autophagic function in different tissues and to develop new mouse models for the study of autophagy in a whole organisms. These animals have been and will keep being essential for performing studies contained in all four projects that will characterize the consequences of the age-related changes in autophagy on different aspects of cell biology and organ and system function. The specific aims of the core are: 1) to generate and maintain genetically modified animals with altered autophagy in different tissues; 2) to maintain age-controlled colonies of wild-type and genetically modified mice; 3) to serve as a link among the investigators in the Program Project by coordinating the sacrifice of animals to maximize their use and by integrating the information obtained from the different animal models by each investigator; 4) to facilitate uniformity in the mouse interventions incorporated in all the projects during this new period; 5) to collect life-span information on some of the transgenic mouse models shared by all projects and integrate the heath-span information from each project in the same animals; 6) to work closely with Core B in the characterization of the novel CMA reporter mouse models. The services offered by the Core include: maintenance of the mouse colonies, coordination of genotyping, administration and monitoring of animal treatments (diets and pharmacological compounds), animal dissection, collection of tissue samples for storage or histopathological analysis and continuous access to a searchable animal data base. The key personnel of the Core are the director, who is assisted by the animal technician. The director supervises all the activities of the Core, approves animal use by the PP members, establishes breeding schemes to accommodate the project needs and oversees animal information input in the database. The technician performs all of the activities related to the maintenance of the animal colonies, genotyping, treatments, tissue collection, behavioral testing and inputs information into the database.

老龄和转基因动物核心(核心C)将继续支持体内实验的需要本项目四个项目的鼠标模型。这个核心的长期目标是为项目提供广泛的年龄控制动物剧目不同组织中具有改良自噬功能的模型和建立新的小鼠模型进行研究整个生物体中的自噬。这些动物过去是，将来也将继续是表演的必需品包含在所有四个项目中的研究将表征与年龄相关的变化的后果细胞生物学以及器官和系统功能的不同方面的自噬。核心的具体目标是：1)产生和维护转基因动物不同组织中的自噬；2)维持野生型和转基因的年龄控制的菌落 MICE；3)作为项目调查人员之间的纽带，协调牺牲通过整合从不同动物模型获得的信息来最大限度地利用动物每名调查员；4)促进纳入所有项目的老鼠干预措施的一致性在这一新时期；5)收集有关一些转基因小鼠模型的生命周期信息通过所有项目，并将来自每个项目的健康跨度信息整合到同一动物中；6)工作与Core B密切相关的新型CMA报告小鼠模型的表征。中心提供的服务包括：维持小鼠群体，协调基因分型，管理和监测动物治疗(饲料和药理化合物)、动物解剖、收集组织样本以进行存储或组织病理学分析，并连续获取可搜索的动物数据库。核心的关键人员是主任，他由动物技术员协助。导演监督核心的所有活动，批准PP成员使用动物，建立育种计划以适应项目需要，并监督数据库中动物信息的输入。这个技术人员执行与动物群体维护、基因分型、治疗、组织收集、行为测试，并将信息输入数据库。