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The Photoreceptor Cilium

光感受器纤毛

基本信息

批准号：
9350346
负责人：
DAVID S WILLIAMS
金额：
$ 38.5万
依托单位：
UNIVERSITY OF CALIFORNIA LOS ANGELES
依托单位国家：
美国
项目类别：
财政年份：
2001
资助国家：
美国
起止时间：
2001-09-30 至 2020-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9350346
关键词：
Address Animal Model Biochemistry Biological Carrier Proteins Cell Culture Techniques Cell Line Cell Survival Cell membrane Cell physiology Cells Cellular biology Cilia Diffusion Disease Elements Endocytosis Epithelial Cells Event Fluorescence Recovery After Photobleaching Genes Genetic Models Goals Golgi Apparatus Guanosine Triphosphate Phosphohydrolases Image Impairment Individual Kinesin Lateral MYO7A gene Measurement Measures Membrane Membrane Proteins Methods Microscopy Molecular Movement Mus Mutant Strains Mice Neck Opsin Pathogenesis Phenotype Photoreceptors Process Protein Sortings Proteins Research Retinal Degeneration Rhodopsin Role Route Site Study models System Testing Time Travel Treatment Efficacy Usher Proteins Vesicle Yeasts base ciliopathy improved innovation insight knock-down live cell imaging mutant mutant mouse model novel preclinical study prevent public health relevance retinal rods trafficking

项目摘要

DESCRIPTION (provided by applicant): The photoreceptor outer segment is an elaborate primary cilium. The delivery of opsin into and along the cilium is critical for photoreceptor cell function and viability. The overall goal of this study is to understand the cellular mechanisms involved in these delivery processes. A combination of cell biological methods, including imaging of protein movements in the cilium of live mouse photoreceptor cells, will be used. Studies will address questions about opsin entry into and transport along the cilium, and the membrane diffusion barrier of the photoreceptor cilium. The proposed research incorporates technical innovation, including the measurements of real-time movements of opsin along the cilium, using a ciliated epithelial cell line and mouse rod photoreceptor cells. It will test novel concepts of opsin trafficking and the organization of the photoreceptor cilium.

描述（由申请人提供）：光感受器外节是一个复杂的初级纤毛。将视蛋白递送到纤毛中并沿着纤毛递送对于感光细胞的功能和活力至关重要。这项研究的总体目标是了解这些传递过程中涉及的细胞机制。将使用细胞生物学方法的组合，包括活体小鼠感光细胞纤毛中蛋白质运动的成像。研究将解决有关视蛋白进入纤毛并沿纤毛运输以及感光器纤毛的膜扩散屏障的问题。拟议的研究融合了技术创新，包括使用纤毛上皮细胞系和小鼠视杆感光细胞测量视蛋白沿纤毛的实时运动。它将测试小说视蛋白运输和光感受器纤毛组织的概念。