Kinesin in photoreceptors cells
Kinesin in photoreceptors cells
批准号:
7382723
负责人:
DAVID S WILLIAMS
金额:
$38.5万
依托单位国家:
美国
项目类别:
财政年份:
2009
资助国家:
美国
项目状态:
已结题
起止时间:
2009-09-30 至 2011-09-29
关键词:
Cell Culture TechniquesCell DeathCell physiologyCellsCellular biologyCharacteristicsCiliaComplexDevelopmentDistalGenesGeneticGenetic ModelsGoalsGrowthIn VitroKinesinKineticsKnockout MiceLeadLifeLightingLinkMembrane ProteinsMicrotubulesModelingMolecularMotorMovementMusMutant Strains MiceOpsinPhotoreceptorsPhysiologicalProtein translocationProteinsRNA InterferenceRetinal DiseasesRoleSuggestionSystemTestingTransport Processbasecellular imagingexperiencein vivoinherited retinal degenerationinsightkinetosomeprotein complexprotein transportresearch studyresponseskillstool
中文摘要
描述(由申请人提供):驱动蛋白是微管马达蛋白,产生许多基本细胞过程所必需的细胞内运动。本项目的长期目标是了解驱动蛋白-2在光感受器纤毛中的功能。目前的应用是基于三个新的发展:(1)一个新建立的模型,视蛋白运输的纤毛,可以通过活细胞成像研究;(2)的发现,已知的视网膜疾病基因链接驱动蛋白-2和纤毛运输;(3)完善的遗传模型,使损失的驱动蛋白-2可以研究感光细胞死亡之前。我们建议利用这些新的发现和发展:(1)测试两个相互竞争的假设,驱动蛋白-2如何产生视蛋白运输沿着纤毛,并测试不同的蛋白质的作用,在运输的视蛋白沿着感光纤毛。(2)确定驱动蛋白-2在与感光器纤毛相关的不同(非视蛋白)运输过程中的贡献。这些研究的结果将导致更好地了解感光器纤毛中的关键细胞过程,从而为一组主要的遗传性视网膜变性提供重要的新见解。
英文摘要
DESCRIPTION (provided by applicant): Kinesins are microtubule motor proteins that generate intracellular movement essential for many fundamental cellular processes. The long-term goal of this project is to understand the function of kinesin-2 in the photoreceptor cilium. The current application is based on three new developments: (1) A newly established model of opsin transport in cilia that can be studied by live-cell imaging; (2) Findings that link known retinal disease genes to kinesin-2 and ciliary transport; and (3) Refinement of the genetic model so that loss of kinesin-2 can be studied prior to photoreceptor cell death. We propose to capitalize upon these new findings and developments by: (1) Testing two competing hypotheses for how kinesin-2 generates opsin transport along the cilium, and testing the roles of different proteins in the transport of opsin along the photoreceptor cilium. (2) Determining the contribution of kinesin-2 in different (non-opsin) transport processes related to the photoreceptor cilium. The results of these studies will lead to a better understanding of critical cellular processes in the photoreceptor cilium, and thus provide important new insight into a major group of inherited retinal degenerations.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Cellular Mechanisms of Photoreceptor Disk Morphogenesis
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批准号:10599986
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项目类别:
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资助金额:$45.89万
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财政年份:2022
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负责人:DAVID S WILLIAMS
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依托单位:
Cellular Mechanisms of Photoreceptor Disk Morphogenesis
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批准号:10442259
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项目类别:
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资助金额:$45.89万
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财政年份:2022
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负责人:DAVID S WILLIAMS
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Exploring the relationship of water flow across the RPE and mutant-MYO7A/Usher 1B
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批准号:9886098
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项目类别:
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资助金额:$23.4万
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财政年份:2020
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负责人:DAVID S WILLIAMS
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依托单位:
RPE cell biology, aging, and disease
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批准号:9765536
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项目类别:
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资助金额:$30.0万
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财政年份:2018
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负责人:DAVID S WILLIAMS
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依托单位:
RPE cell biology, aging, and disease
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批准号:10165720
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项目类别:
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资助金额:$44.92万
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财政年份:2017
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负责人:DAVID S WILLIAMS
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依托单位:
Kinesin in photoreceptors cells
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批准号:7935221
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项目类别:
-
资助金额:$38.5万
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财政年份:2009
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负责人:DAVID S WILLIAMS
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依托单位:
The Photoreceptor Cilium
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批准号:8440269
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项目类别:
-
资助金额:$38.5万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
The Photoreceptor Cilium
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批准号:9350346
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项目类别:
-
资助金额:$38.5万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
KINESIN IN PHOTORECEPTOR CELLS
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批准号:6799946
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项目类别:
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资助金额:$27.83万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
KINESIN IN PHOTORECEPTOR CELLS
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批准号:6654910
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项目类别:
-
资助金额:$27.83万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
KINESIN IN PHOTORECEPTOR CELLS
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批准号:6318855
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项目类别:
-
资助金额:$27.83万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
KINESIN IN PHOTORECEPTOR CELLS
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批准号:6525004
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项目类别:
-
资助金额:$27.83万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
Microscopy and Imaging Analysis Core
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批准号:10430209
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项目类别:
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资助金额:$25.68万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Administrative Core
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批准号:10655341
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项目类别:
-
资助金额:$2.38万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Stein Eye Institute Core Grant for Vision Research
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批准号:9310248
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项目类别:
-
资助金额:$59.05万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Microscopy and Imaging Analysis Core
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批准号:10020827
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项目类别:
-
资助金额:$25.68万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Vision Research Core at UCLA
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批准号:10239003
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项目类别:
-
资助金额:$78.0万
-
财政年份:1997
-
负责人:DAVID S WILLIAMS
-
依托单位:
Microscopy and Imaging Analysis Core
-
批准号:10655342
-
项目类别:
-
资助金额:$25.68万
-
财政年份:1997
-
负责人:DAVID S WILLIAMS
-
依托单位:
Microscopy and Imaging Analysis Core
-
批准号:10239005
-
项目类别:
-
资助金额:$25.68万
-
财政年份:1997
-
负责人:DAVID S WILLIAMS
-
依托单位:
Administrative Core
-
批准号:10430208
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项目类别:
-
资助金额:$2.38万
-
财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
国内基金
海外基金
炎性反应中巨噬细胞激活诱导死亡(activation-induced cell death,AICD)的机理研究
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批准号:30330260
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项目类别:重点项目
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资助金额:105.0万元
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批准年份:2003
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负责人:顾军
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依托单位: