Cellular Mechanisms of Photoreceptor Disk Morphogenesis
Cellular Mechanisms of Photoreceptor Disk Morphogenesis
批准号:
10442259
负责人:
DAVID S WILLIAMS
金额:
$45.89万
依托单位国家:
美国
项目类别:
财政年份:
2022
资助国家:
美国
项目状态:
未结题
起止时间:
2022-05-01 至 2026-03-31
关键词:
AddressAreaC-terminalCell membraneCellsCellular biologyCiliaDefectDiseaseGatekeepingGenetic studyGoalsGrowthHealthHumanImpairmentKnowledgeLengthMaintenanceMembraneMethodsMicroscopyMolecularMorphogenesisMusOpsinPathogenesisPharmacology StudyPhenotypePhotoreceptorsProcessProteinsResearchResolutionRetinal DegenerationRetinal DiseasesRhodopsinRoleShapesSiteTestingThree-dimensional analysisTissuesVertebrate PhotoreceptorsVisualexperimental studyin vivonovelperipherinphotoreceptor discprotein functionretinal rodsrhotomographytool
中文摘要
项目摘要
光感受器纤毛是所有初级纤毛中最精细的。它的质膜广泛地
被放大以形成含有非常高浓度的视觉感受器的盘膜的堆叠,
视蛋白这一堆圆盘形成了感光器外节,其组织对于视觉至关重要
灵敏度和空间分辨率。拟议研究的总体目标是了解细胞
在磁盘膜的形态发生机制。
该研究计划建立在分子工具的应用基础上,使用先进的显微镜,
包括EM断层扫描和活细胞超分辨率显微镜,用于高分辨率,3-D分析。的
这项研究的长期目标是从分子水平上了解感光盘
形态发生,包括将必需蛋白质递送到膜生长位点的机制。
具体目标将解决视蛋白的睫状定位的未知方面,以及磁盘的作用
形态发生蛋白在启动板层生长中的作用,
带有非特化纤毛的细胞培养物。我们的研究结果将提供一个关键领域的理解,
感光细胞生物学它们也将是我们理解视网膜病变发病机制的基础。
由于盘膜形态发生的紊乱而引起的疾病。
英文摘要
Project Summary
The photoreceptor cilium is the most elaborate of all primary cilia. Its plasma membrane is extensively
amplified to form a stack of disk membranes that contain a very high concentration of the visual receptor,
opsin. This stack of disks forms the photoreceptor outer segment, and its organization is central to visual
sensitivity and spatial resolution. The overall goal of the proposed research is to understand the cellular
mechanisms involved in the morphogenesis of the disk membranes.
The research plan is built on the application of molecular tools, with the use of advanced microscopy,
including EM tomography and live-cell super-resolution microscopy, for high resolution, 3-D analysis. The
long-term goal of the proposed research is to provide a molecular understanding of photoreceptor disk
morphogenesis, including the mechanisms for delivering essential proteins to the site of membrane growth.
The Specific Aims will address unknown aspects of the ciliary localization of opsin, and the roles of disk
morphogenetic proteins in initiating lamellar growth, using both mouse rod photoreceptors in vivo and
cultures of cells bearing unspecialized cilia. Our findings will provide understanding of a key area of
photoreceptor cell biology. They will also be fundamental to our understanding of the pathogenesis of retinal
disease that ensues from perturbations of disk membrane morphogenesis.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Cellular Mechanisms of Photoreceptor Disk Morphogenesis
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批准号:10599986
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项目类别:
-
资助金额:$45.89万
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财政年份:2022
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负责人:DAVID S WILLIAMS
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依托单位:
Exploring the relationship of water flow across the RPE and mutant-MYO7A/Usher 1B
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批准号:9886098
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项目类别:
-
资助金额:$23.4万
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财政年份:2020
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负责人:DAVID S WILLIAMS
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依托单位:
RPE cell biology, aging, and disease
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批准号:9765536
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项目类别:
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资助金额:$30.0万
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财政年份:2018
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负责人:DAVID S WILLIAMS
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依托单位:
RPE cell biology, aging, and disease
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批准号:10165720
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项目类别:
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资助金额:$44.92万
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财政年份:2017
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负责人:DAVID S WILLIAMS
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依托单位:
Kinesin in photoreceptors cells
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批准号:7382723
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项目类别:
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资助金额:$38.5万
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财政年份:2009
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负责人:DAVID S WILLIAMS
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依托单位:
Kinesin in photoreceptors cells
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批准号:7935221
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项目类别:
-
资助金额:$38.5万
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财政年份:2009
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负责人:DAVID S WILLIAMS
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依托单位:
The Photoreceptor Cilium
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批准号:8440269
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项目类别:
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资助金额:$38.5万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
The Photoreceptor Cilium
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批准号:9350346
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项目类别:
-
资助金额:$38.5万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
KINESIN IN PHOTORECEPTOR CELLS
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批准号:6799946
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项目类别:
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资助金额:$27.83万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
KINESIN IN PHOTORECEPTOR CELLS
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批准号:6654910
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项目类别:
-
资助金额:$27.83万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
KINESIN IN PHOTORECEPTOR CELLS
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批准号:6318855
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项目类别:
-
资助金额:$27.83万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
KINESIN IN PHOTORECEPTOR CELLS
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批准号:6525004
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项目类别:
-
资助金额:$27.83万
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财政年份:2001
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负责人:DAVID S WILLIAMS
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依托单位:
Microscopy and Imaging Analysis Core
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批准号:10430209
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项目类别:
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资助金额:$25.68万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Administrative Core
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批准号:10655341
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项目类别:
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资助金额:$2.38万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Stein Eye Institute Core Grant for Vision Research
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批准号:9310248
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项目类别:
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资助金额:$59.05万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Microscopy and Imaging Analysis Core
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批准号:10020827
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项目类别:
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资助金额:$25.68万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Vision Research Core at UCLA
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批准号:10239003
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项目类别:
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资助金额:$78.0万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Microscopy and Imaging Analysis Core
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批准号:10655342
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项目类别:
-
资助金额:$25.68万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Microscopy and Imaging Analysis Core
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批准号:10239005
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项目类别:
-
资助金额:$25.68万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
Administrative Core
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批准号:10430208
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项目类别:
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资助金额:$2.38万
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财政年份:1997
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负责人:DAVID S WILLIAMS
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依托单位:
国内基金
海外基金
层出镰刀菌氮代谢调控因子AreA 介导伏马菌素 FB1 生物合成的作用机理
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批准号:2021JJ40433
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项目类别:省市级项目
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资助金额:--
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批准年份:2021
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负责人:孙磊
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依托单位:
寄主诱导梢腐病菌AreA和CYP51基因沉默增强甘蔗抗病性机制解析
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批准号:32001603
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项目类别:青年科学基金项目
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资助金额:24.0万元
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批准年份:2020
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负责人:段真珍
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依托单位:
AREA国际经济模型的移植.改进和应用
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批准号:18870435
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项目类别:面上项目
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资助金额:2.0万元
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批准年份:1988
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负责人:史树中
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依托单位: