Comprehensive Genomics Shared Facility

分子分析/翻译研究小组 综合基因组学共享设施(CGSF) 摘要 综合基因组学共享设施(CGSF)的目标是为癌症中心的研究人员提供 获得尖端基因组资源和旨在促进和加强质量的方法 癌症相关研究中的遗传学、基因组和分子研究。共享的全面基因组学 设施通过两个相互关联的组件实现这一目标：园区内的UAB下一代和 桑格测序(NGSS)ARM和哈德逊阿尔法生物技术研究所(HAIB)的下一代 和临床测序臂。这一安排为UAB癌症中心成员提供了前所未有的 获得最先进的遗传和基因组分析技术，用于基础研究和临床研究。 每个组件都为中心成员带来了宝贵的资源。NGSS提供现场咨询和 为需要不同类型基因组技术的各种项目提供服务。其中包括标准 Sanger测序、人类细胞系鉴定(包括癌细胞系)、SNP基因分型分析和 下一代测序服务(RNA-Seq、ChIP-Seq、MicroRNA-Seq、Exome-Seq、定制扩增、 微生物组、元基因组学和线粒体测序)。HAIB组件提供非常高的吞吐量 为生产级全基因组测序提供服务，并为开发和常规处理提供支持 需要CLIA环境或受益于CLIA环境的临床样本。CGSF支持基础研究人员 探讨肿瘤发生、发展、转移的机制及临床意义 研究人员追求精确医学方法，并提供他们所需的工具，以高效和 有效地为癌症研究领域贡献了重大发现。

MOLECULAR ANALYSIS / TRANSLATION RESEARCH GROUP COMPREHENSIVE GENOMICS SHARED FACILITY (CGSF) ABSTRACT The goal of the Comprehensive Genomics Shared Facility (CGSF) is to provide Cancer Center investigators access to cutting-edge genomic resources and methodologies designed to facilitate and strengthen the quality of genetic, genomic, and molecular studies in cancer related research. The Comprehensive Genomics Shared Facility accomplishes this goal through two interrelated components: the on campus UAB Next-Generation and Sanger Sequencing (NGSS) arm, and the HudsonAlpha Institute for Biotechnology (HAIB) Next-Generation and Clinical Sequencing arm. This arrangement provides UAB Cancer Center members unprecedented access to state-of-the-art technologies for genetic and genomic analysis for basic research and clinical studies. Each component brings valuable resources to center members. The NGSS provides onsite consultation and service for various projects that require different types of genomic technologies. These include standard Sanger sequencing, human cell line identification (including cancer cell lines), SNP genotyping assays and Next-Generation Sequencing services (RNA-Seq, ChIP-Seq, microRNA-Seq, Exome-Seq, Custom amplicons, Microbiome, Metagenomics, and mitochondrial sequencing). The HAIB component offers very high throughput services for production-level whole genome sequencing and support for development and routine processing of clinical samples that require or benefit from a CLIA environment. The CGSF supports basic researchers investigating the mechanisms of cancer development, progression and metastasis as well as clinical researchers pursuing precision medicine approaches and provides the tools they need to efficiently and effectively contribute significant findings to the field of cancer research.