Using Quantitative Proteomics to Elucidate the Signaling Consequences of Microtubule Disruption

使用定量蛋白质组学阐明微管破坏的信号传导后果

基本信息

  • 批准号:
    9051090
  • 负责人:
  • 金额:
    $ 3.72万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-03-01 至 2019-02-28
  • 项目状态:
    已结题

项目摘要

 DESCRIPTION (provided by applicant): Microtubules are large cellular structures that grow and shrink on the order of seconds to minutes. Chemical disruption of microtubule dynamics typically leads to death in both cycling and non-cycling cells, though the mechanisms responsible for this are not well characterized. Interestingly, microtubule drugs stimulate kinase- mediated stress signaling prior to cell death, which suggests that cells can detect and respond to microtubule disruption. I propose to address the questions surrounding this intriguing stress phenomenon. First, I would like to understand the general impact that microtubule perturbations have on cellular signaling. Given the precedent of phosphorylation stress signals in response to microtubule disruption, I propose to generate highly quantitative mass spectrometry measurements of protein phosphorylation that occurs immediately following microtubule drug treatment. Second, I want to determine whether different microtubule perturbations (e.g. stabilization and destabilization) have different effects on cellular signaling. To do this, I willuse different drugs to perturb microtubules and compare effects on protein phosphorylation. Finally, there is currently very little knowledge regarding how microtubules might directly regulate signaling, especially in response to chemical insults. I hope to elucidate mechanisms by which signaling proteins (e.g. kinases, phosphatases) are regulated by microtubule dynamics and activated in response to microtubule disruption. To this end, I will use immunofluorescence imaging, conventional transfection techniques, and statistical inference methods.


项目成果

期刊论文数量(0)
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Javier Jesus Pineda其他文献

Javier Jesus Pineda的其他文献

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{{ truncateString('Javier Jesus Pineda', 18)}}的其他基金

Using Quantitative Proteomics to Elucidate the Signaling Consequences of Microtubule Disruption
使用定量蛋白质组学阐明微管破坏的信号传导后果
  • 批准号:
    9237119
  • 财政年份:
    2016
  • 资助金额:
    $ 3.72万
  • 项目类别:

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