Protein microarray antibody responses to P. falciparum in a human challenge model
人类攻击模型中对恶性疟原虫的蛋白质微阵列抗体反应
基本信息
- 批准号:9314780
- 负责人:
- 金额:$ 18.3万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2017
- 资助国家:美国
- 起止时间:2017-02-06 至 2022-01-31
- 项目状态:已结题
- 来源:
- 关键词:AffinityAmino Acid SequenceAmino AcidsAntibodiesAntibody Binding SitesAntibody FormationAntibody ResponseAntigensBerryBindingBiometryBiteCharacteristicsClinical ResearchCulicidaeDataDevelopmentDevelopment PlansDoctor of MedicineEpidemiologyEpitopesEvolutionExposure toFalciparum MalariaFundingGenetic PolymorphismGenetic VariationGoalsGrantHumanImmune responseImmune systemImmunityImmunoglobulin GImmunoglobulin MImmunologic MemoryIndividualInfectionKnowledgeLicensureMalariaMalaria VaccinesMalaria preventionMapsMarylandMeasuresMentorsMentorshipMicroarray AnalysisModelingMorbidity - disease rateOutcomeParasitesParticipantPatternPediatricsPeptidesPlasmodium falciparumPositioning AttributeProtein MicrochipsProteinsResearchResearch PersonnelScanningSerologicalSystemTrainingTranslational ResearchUniversitiesVaccine DesignVaccinesVariantVolunteer GroupWhole OrganismWorkcareer developmentcross reactivitydesignexperiencefield studygenetic makeupimprovedinsightmalaria infectionmedical schoolsmortalitypolyclonal antibodyprofessorresponsevaccine developmentvaccine efficacyvolunteer
项目摘要
PROJECT SUMMARY
This K23 application is submitted by Andrea A. Berry, M.D., an Assistant Professor of Pediatrics at the
University of Maryland School of Medicine. Dr. Berry's long term goal is to become an independent investigator
in the field of malaria translational research. Towards this goal, she proposes a mentored career development
plan that provides training in microarray analysis, biostatistics, and epidemiology.
An effective Plasmodium falciparum malaria vaccine will boost prospects for the control and eventual
eradication of malaria. However, the field is only beginning to understand the requirements and characteristics
of malaria protective immunity, which is key to vaccine development. A promising approach is serological
profiling on high-throughput protein microarrays, which can elucidate antibody responses to hundreds of P.
falciparum antigen variants simultaneously. The investigator's group uses protein microarrays containing
diverse variants of key P. falciparum antigens so that they can study antigen variants associated with cross-
reactive and cross-protective immune responses. However, a gap in knowledge is the ability to differentiate
between antibody binding due to cross-reactivity and antibody binding due to accumulated exposure to malaria
– on protein microarrays, both responses manifest as reactivity to multiple antigen variants. Differentiating
these two patterns of antibody binding is difficult in field studies because only limited data is available on
participants' previous exposure, and even for ongoing infections one cannot always know the exact genetic
makeup of the parasite or the exact timing of exposure. Elucidating the characteristics of cross-reactivity and
accumulated exposure is nonetheless critical to gaining a better understanding of the development of
protective immunity from malaria.
Controlled Human Malaria Infections, in which volunteers are exposed to malaria through the bites of infectious
mosquitoes, are an opportunity to study the effect of single-clone malaria infections on the immune response.
This proposed work will evaluate immune responses in malaria naïve volunteers who experience single and
repeated P. falciparum infections in order to inform the interpretation of immune responses in individuals living
in malaria endemic regions. Protein and peptide microarrays will be used to probe sera from initially naïve
volunteers after they have recovered from P. falciparum challenge.
Aim 1: Evaluate the effect of single infections on the immune response by comparing two groups of volunteers
after infection with two different P. falciparum strains.
Aim 2: Evaluate the effect of repeated infection on the immune response by following a group of volunteers
who experience four P. falciparum infections over two years.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Andrea Berry其他文献
Andrea Berry的其他文献
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{{ truncateString('Andrea Berry', 18)}}的其他基金
Multiplex analysis of IgA and IgG antibody responses to early childhood malaria infections to inform vaccine development
对儿童早期疟疾感染的 IgA 和 IgG 抗体反应进行多重分析,为疫苗开发提供信息
- 批准号:
10647960 - 财政年份:2023
- 资助金额:
$ 18.3万 - 项目类别:
Protein microarray antibody responses to P. falciparum in a human challenge model
人类攻击模型中对恶性疟原虫的蛋白质微阵列抗体反应
- 批准号:
10092899 - 财政年份:2017
- 资助金额:
$ 18.3万 - 项目类别:
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