Scientific Core B Cardiac Imaging Core

Scientific Core B 心脏成像核心

• 批准号: 9208532
• 负责人: MICHAEL RUBART-VAN DER LOHE
• 金额: $ 39万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-02-15 至 2022-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9208532
• 关键词: Address; Area; Cardiac; Cardiac Myocytes; Cardiac development; Cardiomyopathies; Computer software; Confocal Microscopy; Congenital Heart Defects; Data; Defect; Detection; Development; Dimensions; Disease; Doctor of Medicine; Echocardiography; Embryo; Equipment; Evaluation; Frequencies; Functional Imaging; Goals; Growth; Health; Heart; Human; Image; Image Analysis; Lasers; Longitudinal Studies; Microscope; Microscopy; Mission; Morphogenesis; Mus; Mutation; National Heart, Lung, and Blood Institute; Optics; Pattern; Performance; Prevention; Primitive Streaks; Publishing; Research; Research Proposals; Resolution; Scanning; Services; Shunt Device; Structure; Techniques; Technology; Tissue imaging; Transducers; Ultrasonography; United States National Institutes of Health; Ventricular; base; cardiovascular visualization; detector; experience; fetal; fluorescence imaging; heart imaging; hemodynamics; in vivo; postnatal; programs; public health relevance; quantitative imaging; success

ABSTRACT The Cardiac Imaging Core (Core B) will provide service to all 3 projects with the goals of generating confocal microscopy- and in vivo ultrasound – based analyses of cardiac development, structure, and function. Core B will be directed by Michael Rubart, M.D., who will provide expertise in confocal imaging of tissues. Dr. Rubart will be assisted by Ronald Payne, M.D., who will provide expertise in ultrasound imaging of embryonic and postnatal cardiac development and function. Core B's services enhance the efficiency of the Projects by providing consistent expertise in the capture and evaluation of images from tissue sections and hearts in vivo, using confocal microscopy and echocardiography, respectively. There are 2 Specific Aims for the Core: Aim 1 will provide high-quality, unbiased confocal fluorescence imaging of the pre- and postnatal heart, as well as quantitative image analyses. Approaches utilized in Aim 1 will quantitatively assess (i) primitive streak formation and node polarity (Project 1), (ii) sarcomeric organization and cardiomyocyte polarity (Project 1-3), and (iii) ventricular wall trabeculation and compaction (Project 3). Imaging will be performed using a laser scanning confocal microscope equipped with multiple laser excitation lines and detector channels, as well as a variety of high/low magnification, high/low numerical aperture objectives. Aim 2 will provide high-resolution in vivo ultrasound imaging of the pre- and postnatal heart in Projects 1-3 in order to generate quantitative data of structure, function, and flow relative to genetic alterations in cardiac development. In addition, ultrasound imaging will allow the study of longitudinal structural and functional data. Ultrasound imaging will utilize probes with frequency bands from 13 to 70 MHz, and linear array transducer technology, enabling imaging of hearts at different stages of development and frame acquisition at high rates, respectively. Available analysis software will enable quantification of cardiac contractile performance, chamber dimensions, and flow pattern, including detection and size estimation of intracardiac shunts. Overall, technologies utilized in the Cardiac Imaging Core will enable unbiased, quantitative assessments of changes in cardiac structure and function as a result of genetic alterations studied in each of the three Projects of this application.

摘要 心脏成像核心(核心B)将为所有3个项目提供服务，目标是产生共焦 基于显微镜和体内超声的心脏发育、结构和功能分析。核心B 将由迈克尔·鲁巴特医学博士指导，他将提供组织共焦成像方面的专业知识。鲁巴特博士 将由罗纳德·佩恩医学博士协助，他将提供胚胎和 出生后心脏发育和功能。核心B的服务通过以下方式提高项目的效率 在活体组织切片和心脏图像的捕获和评估方面提供一致的专业知识， 分别使用共聚焦显微镜和超声心动图。核心有两个具体目标：目标1 将为出生前和出生后的心脏提供高质量、无偏的共聚焦荧光成像，以及 定量图像分析。目标1中使用的方法将定量评估(I)原始条纹 形成和结极性(项目1)，(2)肌节组织和心肌细胞极性(项目1-3)， 和(Iii)室壁小梁和致密化(项目3)。成像将使用激光进行 配备多条激光激发线和探测器通道的扫描共聚焦显微镜，以及 各种高/低放大倍率、高/低数值孔径物镜。AIM 2将提供高分辨率 在项目1-3中对出生前和出生后的心脏进行活体超声成像，以便产生定量数据 心脏发育中与遗传变化相关的结构、功能和血流。此外，超声波 成像将允许研究纵向结构和功能数据。超声成像将利用探头 具有13至70 MHz的频段，以及线性阵列换能器技术，使心脏成像能够在 不同的发展阶段和高速率的帧获取。可用的分析软件 将能够量化心脏收缩性能、心腔尺寸和血流模式，包括 心内分流的检测和大小估计。总体而言，心脏成像核心中使用的技术 将能够对心脏结构和功能的变化进行公正、定量的评估 本申请的三个项目中的每个项目都研究了基因改变。