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The ocular surface microbiome in potentially infectious ophthalmic disease

潜在传染性眼科疾病中的眼表微生物组

基本信息

批准号：
9185320
负责人：
Cecilia Sungmin Lee
金额：
$ 19.91万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-12-01 至 2019-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9185320
关键词：
Aftercare Archives Artificial Tears Biopsy Blindness Characteristics Clinical Clinical Research Clinical Trials Communicable Diseases Communities Complex Computer Simulation Conjunctivitis Cyclosporine DNA DNA sequencing Detection Development Disease Disease Outcome Dry Eye Syndromes Endophthalmitis Epidemic Keratoconjunctivitis Epidemiology Eye Eye Infections Eye diseases Goals Health Infection Institution K-Series Research Career Programs Karyotype determination procedure Keratitis Lead Master of Science Measures Mentors Mentorship Methods Microbiology Morbidity - disease rate Ophthalmology Organism Outcome Pathogenesis Patients Physicians Physiological Randomized Randomized Clinical Trials Research Research Personnel Resolution Retrospective Studies Role Sampling Scientist Severity of illness Signs and Symptoms Structure Swab Techniques Training Transfusion-Transmitted Virus Treatment outcome Viral Virus Visual Visual Acuity arm base career cohort experience microbial microbiome molecular diagnostics novel ocular surface pathogen prospective public health relevance treatment group treatment response

项目摘要

DESCRIPTION (provided by applicant): Ocular infectious diseases such as endophthalmitis and conjunctivitis remain a significant cause of visual morbidity and potential blindness. However, traditional microbial cultures often fail to identify causative organisms in a high proportion of clinically suspicious infections. Most pathogens responsible for ocular infections originate from the ocular surface. Thus, characterizing the ocular surface microbiome in different disease states may lead to a better understanding of the pathogenesis of these conditions. Our goal is to understand the community structure of ocular surface microbial flora that predisposes the eye to infection and influences various disease courses. Our overarching hypothesis is that the constituents of the ocular surface microbiome are involved in the pathogenesis of potentially infectious ophthalmic conditions. To explore this hypothesis, we propose to study the ocular surface microbiome, in particular a recently described virus, torque teno virus (TTV), in three diseases by using a novel, deep DNA sequencing technique. In specific aim 1, we propose one prospective and one retrospective study to determine the correlation between the ocular surface microbiome and clinical outcomes of epidemic keratoconjunctivitis and endophthalmitis. In specific aim 2, we propose a randomized clinical trial to study the ocular surface microbiome and its relationship to the treatment outcome in patients with dry eye syndrome. My ultimate career goal is to become an independent physician-scientist in the field of ophthalmology. I am committed to developing a clinical research-oriented career in major academic institutions and understand the need for me to pursue additional training and mentorship. During the Mentored Research Career Development Award period, I plan to obtain a Master of Science in Epidemiology (Clinical Research Track) and participate in several, focused didactic coursework in microbiology, fundamentals of clinical research, and molecular diagnostics. The additional training, research experience, and most importantly, mentorship by committed experts will be of uttermost importance in becoming a successful clinician-researcher.