The human temporal bone laboratory resource for basic and applied inner ear sciences

用于基础和应用内耳科学的人类颞骨实验室资源

• 批准号: 9537912
• 负责人: Akira Ishiyama
• 金额: $ 15.5万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-01 至 2021-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9537912
• 关键词: Animal Model; Animals; Archives; Auditory; Automobile Driving; Autopsy; Basic Science; Biological Preservation; Biology; Bone Tissue; Celloidin; Chicago; Clinical Research; Collaborations; Collection; Communities; Databases; Detection; Development; Ear; Edema; Funding; Future; Goals; Guidelines; Harvest; Histopathology; Human; Human Resources; Image; Imagery; Immunofluorescence Immunologic; Improve Access; In Situ; In Situ Hybridization; Institution; Instruction; Laboratories; Labyrinth; Liquid substance; Magnetic Resonance Imaging; Maintenance; Medical center; Messenger RNA; Methodology; Methods; Microdissection; Modernization; Molecular; National Institute on Deafness and Other Communication Disorders; Otolaryngology; Pathology; Pharmacotherapy; Preparation; Process; Production; Proteins; Proteomics; Protocols documentation; Registries; Research; Research Personnel; Research Proposals; Resolution; Resources; Scanning Electron Microscopy; Science; Scientist; Site; Specimen; Specimen Handling; Staining method; Stains; Structure; Students; Techniques; Temporal bone structure; Time; Tissue Banks; Tissue membrane; Tissues; Training; Transmission Electron Microscopy; Universities; Update; bone imaging; cost; graduate student; human tissue; improved; in vivo; interest; medical schools; middle ear; new technology; skills; tissue preparation; tool; translational study; transmission process

ABSTRACT In the present proposal we will preserve, process and make available human ear tissue specimens for inner ear basic science researchers such that they may be able to compare with their animal models. The use of celloidin and microdissected human temporal bone tissue for immunohistochemical and quantitative techniques will be instrumental in driving basic animal inner ear research forward towards understanding of human ear pathology and has high potential for the development of effective drug therapies for restoring auditory and vestibular function. In Specific Aim 1) we will improve access for the auditory and vestibular research community to human ear tissues from temporal bone specimens. We will improve and promote timely procurement, preparing and processing of new specimens, and appropriate re-processing of archived specimens. We will distribute a limited number of well-prepared laboratory tissues to funded researchers who lack adequate access to facilities for human temporal bone preparation. We will also exploit use of the Registry for information about currently archived tissues and their access by the community, and promote collaborations between researchers seeking to validate animal models for human relevance. Specific Aim 2) We will optimize tissue preparation techniques. We will include molecular and cellular marking and visualization techniques that allow direct comparisons of fresh and archived human tissues with those from animal models. We will disseminate protocols for procurement, preservation and preparation of human ear tissues from temporal bones. By doing this we will encourage animal researchers to include human tissues into their analyses. Specific Aim 3) We will use high resolution MRI to evaluate the fluid-filled inner ear structures, and ex vivo MRI to evaluate the inner ear structures post-mortem and make comparisons with celloidin- embedded histopathology. We will exploit new technologies and methods to image and visualize human middle and inner ears in situ with the aim of visualizing soft cellular tissues, membranes and fluids, as well as hard bony tissues. Specific Aim 4) We will provide technical instruction with hands-on training will be available for investigators, technicians otolaryngology residents, medical school and graduate students interested in inner ear biology in the temporal bone processing techniques and skills needed for processing human ear tissues from temporal bones. The materials generate in this proposal will be mutually beneficial for researchers in basic scientific studies of the inner ear, as well as to temporal bone scientists wishing to apply high levels of molecular and cellular techniques to the human temporal bone. These collaborative efforts hold promise to rapidly accelerate the science of temporal bone processing and the understanding of human otopathologies.

摘要 在本提案中，我们将保存、处理和提供人耳组织标本， 耳基础科学研究人员，使他们能够与他们的动物模型进行比较。使用 火棉胶和显微解剖的人颞骨组织用于免疫组织化学和定量 技术将有助于推动基础动物内耳研究， 人耳病理学，并具有很高的潜力，发展有效的药物治疗， 听觉和前庭功能。在具体目标1）中，我们将改善听觉和前庭的通道 从颞骨标本中提取人耳组织。我们将及时改进和推广 新标本的采购、制备和处理，以及存档标本的适当再处理 标本我们将向受资助的研究人员分发数量有限的精心制备的实验室组织， 缺乏足够的人颞骨准备设施。我们还将利用 登记处，了解目前存档的组织及其社区访问的信息，并促进 研究人员之间的合作，旨在验证动物模型与人类的相关性。具体目标2） 我们将优化组织制备技术。我们将包括分子和细胞标记， 可视化技术，允许直接比较新鲜和存档的人体组织与来自 动物模型我们将传播获取、保存和准备人耳的规程， 颞骨组织通过这样做，我们将鼓励动物研究人员将人类组织纳入 他们的分析。具体目标3）我们将使用高分辨率MRI来评估充满液体的内耳结构， 和离体MRI以评估死后内耳结构，并与火棉胶进行比较， 包埋组织病理学我们将探索新的技术和方法来成像和可视化人类 中耳和内耳原位，目的是可视化软细胞组织，膜和液体，以及 硬骨组织具体目标4）我们将提供技术指导，并提供实践培训 对于研究人员、耳鼻喉科住院医师、医学院和研究生感兴趣的 内耳生物学中颞骨加工技术和人耳加工所需技能 颞骨组织在这个提议中产生的材料将对研究人员互惠互利 内耳的基础科学研究，以及颞骨科学家希望应用高水平的 用分子和细胞技术对人类颞骨进行研究。这些合作努力有望 快速加速颞骨加工科学和人类耳病理学的理解。