The creation of a digital shareable archive of high-resolution images of the human temporal bone tissue

创建人类颞骨组织高分辨率图像的数字共享档案

• 批准号: 10451412
• 负责人: Akira Ishiyama
• 金额: $ 22.46万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-01 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10451412
• 关键词: Animals; Archives; Auditory; Automobile Driving; Autopsy; Axon; Basic Science; Bone Tissue; Celloidin; Charge; Chicago; Clinical; Clinical Research; Cochlea; Collaborations; Collection; Communities; Databases; Dendrites; Development; Digital Libraries; Ear; Endolymphatic duct; Equipment; Eustachian Tube; External auditory canal; Funding; Goals; Grant; Hematoxylin and Eosin Staining Method; Histologic; Histology; Histopathology; Human; Image; Imaging Techniques; Improve Access; Laboratories; Labyrinth; Light; Morphology; National Institute on Deafness and Other Communication Disorders; Otolaryngology; Paper; Pathology; Pharmacotherapy; Records; Registries; Research; Research Personnel; Resolution; Science; Scientist; Slide; Specialist; Specimen; Stains; Structure; System; Techniques; Temporal bone structure; Time; Tissues; Training; Universities; Validation; digital; digital imaging; endolymphatic sac; high resolution imaging; human imaging; imager; imaging system; middle ear; parent grant; preservation; tissue preparation; tool

ABSTRACT In the present supplement to the U24 grant 1U24DC015910 for the NIDCD National Temporal Bone Laboratory at UCLA, we aim to make available human inner ear histopathological images for educational and scientific purposes. The specific aim of this supplement to our U24 is the creation of a high-resolution digital archive of the NIDCD National Temporal Bone specimens. Temporal bone H&E digitized images will be shared to the NIDCD Registry and Database. The NIDCD National Temporal Bone Laboratory at UCLA houses one of the largest collections of archival temporal bones (approximately 1200 pairs) in USA. Under the U24 Human Temporal Bone grant, the Laboratory has actively pursued the preservation and acquisition of temporal bones and has collaborated with many basic scientists and clinician scientists. We have met the specific aims of the U24 parent grant: 1) to improve access for the auditory and vestibular research community to human inner ear tissues 2) Optimization of tissue preparation techniques 3) the development of imaging techniques to visualize human middle and inner ear and 4) the training of residents and scientists in the study of the human temporal bone histopathology. The UCLA archival human temporal bone specimens are unique since many of our specimens were obtained with minimal postmortem time allowing for superb morphological preservation. In order to disseminate the human temporal bone histopathology as an educational tool, and as a means of collaboration with scientists, we submit the present request for supplement to the NIDCD National Temporal Bone Laboratory at UCLA for specialized equipment to allow for the creation of high-resolution digital library. The use of celloidin-embedded human temporal bones has been instrumental in driving basic animal inner ear research into the understanding of human ear pathology and has a high potential for the development of effective drug therapies for restoring auditory and vestibular function. The materials generated in this proposal aids research scientists who are conducting animal studies in auditory and vestibular research by providing validation of their findings in the human. The imaging system requested in the supplement will allow to create a high-resolution digital image of hematoxylin and eosin sections of all the temporal bones available in our laboratory. These digital images will be uploaded to the National Temporal Bone registry and database.

抽象的 在目前的U24授予1U24DC015910的补充中，NIDCD国家颞骨 加州大学洛杉矶分校的实验室，我们旨在为教育和 科学目的。该补充对我们U24的具体目的是创建高分辨率数字 NIDCD国家颞骨标本的档案。颞骨H＆E数字化图像将共享 到NIDCD注册表和数据库。 UCLA的NIDCD国家颞骨实验室房屋之一 美国最大的档案颞骨（约1200对）集合。在U24人 颞骨赠款，实验室积极追求暂时骨骼的保存和获取 并与许多基础科学家和临床医生合作。我们已经满足了 U24父母赠款：1）提高听觉和前庭研究社区的访问权限 组织2）组织制备技术的优化3）成像技术的发展以可视化 人类中耳和内耳以及4）在人类时间研究中对居民和科学家的培训 骨组织病理学。 UCLA存档人类颞骨标本是独一无二的 用最小的验尸时间获得了标本，以允许出色的形态保存。在 为了将人类颞骨组织病理学传播为一种教育工具，并将其作为一种手段 与科学家合作，我们向NIDCD国家时间提交了当前的补充请求 UCLA的骨实验室使用专用设备，以创建高分辨率数字图书馆。 使用类似素的人颞骨的使用对驱动基本动物内耳有用 研究对人耳朵病理学的理解，并具有很高的发展 恢复听觉和前庭功能的有效药物疗法。本提案中产生的材料 艾滋病研究科学家正在通过提供听觉和前庭研究的动物研究 验证他们在人类中的发现。补充中要求的成像系统将允许创建一个 苏木精的高分辨率数字图像和我们在我们的所有颞骨骨骼中可用 实验室。这些数字图像将上传到国家颞骨注册表和数据库。