The creation of a digital shareable archive of high-resolution images of the human temporal bone tissue

摘要 在本U24补助金1U24DC015910的补充中，NIDCD国家颞骨 实验室在加州大学洛杉矶分校，我们的目标是提供人类内耳组织病理学图像的教育和 科学目的。这个补充我们的U24的具体目的是创造一个高分辨率的数字 NIDCD国家颞骨标本档案。将共享颞骨H&E数字化图像 NIDCD登记和数据库。加州大学洛杉矶分校的NIDCD国家颞骨实验室拥有一个 最大的档案颞骨收藏（约1200对）在美国。U24人类 颞骨研究资助计划，化验所积极进行保存及取得颞骨的工作， 并与许多基础科学家和临床科学家合作。我们已经达到了 U24家长补助金：1）改善听觉和前庭研究社区对人类内耳的访问 2）组织制备技术的优化3）成像技术的发展， 人类中耳和内耳，以及4）培训居民和科学家研究人类颞叶 骨组织病理学加州大学洛杉矶分校的人类颞骨标本档案是独一无二的，因为我们的许多 标本是在最短的死后时间内获得的，允许极好的形态保存。在 为了传播人类颞骨组织病理学作为教育工具，并作为一种手段， 通过与科学家的合作，我们提交了对NIDCD国家时间表的补充申请。 骨实验室在加州大学洛杉矶分校的专门设备，以允许创建高分辨率的数字图书馆。 使用纤维素包埋的人类颞骨在驱动基本的动物内耳方面发挥了重要作用 研究人类耳部病理学的理解，并具有很高的发展潜力， 有效的药物治疗恢复听觉和前庭功能。本提案中生成的材料 艾滋病研究科学家正在进行听觉和前庭研究的动物研究， 验证他们在人类身上的发现。补充资料中要求的成像系统将允许创建 高分辨率数字图像的苏木精和伊红切片的所有颞骨可在我们的 实验室这些数字图像将上传到国家颞骨登记处和数据库。

ABSTRACT In the present supplement to the U24 grant 1U24DC015910 for the NIDCD National Temporal Bone Laboratory at UCLA, we aim to make available human inner ear histopathological images for educational and scientific purposes. The specific aim of this supplement to our U24 is the creation of a high-resolution digital archive of the NIDCD National Temporal Bone specimens. Temporal bone H&E digitized images will be shared to the NIDCD Registry and Database. The NIDCD National Temporal Bone Laboratory at UCLA houses one of the largest collections of archival temporal bones (approximately 1200 pairs) in USA. Under the U24 Human Temporal Bone grant, the Laboratory has actively pursued the preservation and acquisition of temporal bones and has collaborated with many basic scientists and clinician scientists. We have met the specific aims of the U24 parent grant: 1) to improve access for the auditory and vestibular research community to human inner ear tissues 2) Optimization of tissue preparation techniques 3) the development of imaging techniques to visualize human middle and inner ear and 4) the training of residents and scientists in the study of the human temporal bone histopathology. The UCLA archival human temporal bone specimens are unique since many of our specimens were obtained with minimal postmortem time allowing for superb morphological preservation. In order to disseminate the human temporal bone histopathology as an educational tool, and as a means of collaboration with scientists, we submit the present request for supplement to the NIDCD National Temporal Bone Laboratory at UCLA for specialized equipment to allow for the creation of high-resolution digital library. The use of celloidin-embedded human temporal bones has been instrumental in driving basic animal inner ear research into the understanding of human ear pathology and has a high potential for the development of effective drug therapies for restoring auditory and vestibular function. The materials generated in this proposal aids research scientists who are conducting animal studies in auditory and vestibular research by providing validation of their findings in the human. The imaging system requested in the supplement will allow to create a high-resolution digital image of hematoxylin and eosin sections of all the temporal bones available in our laboratory. These digital images will be uploaded to the National Temporal Bone registry and database.