The Human Temporal Bone Laboratory Resource for Basic and Applied Inner Ear Sciences

摘要 在目前的提案中，我们将保存、加工并使人耳组织可用于内耳基础 研究人员将能够与他们的动物模型进行比较。火棉胶的使用，显微解剖的组织 因为免疫组织化学和定量技术将有助于驱动基本的动物内耳 研究对人类耳部病理的认识和开发具有很高的潜力和效果 恢复听觉和前庭功能的药物疗法。在具体目标1)中，我们将改善对 听觉和前庭研究界从人耳的颞骨标本中提取组织。我们会 改进和促进新标本的及时采购、准备和加工，并适当重新 存档标本的处理。我们将把有限数量的精心准备的实验室组织分发给 资助那些无法充分利用人类颞骨准备设施的研究人员。我们还将 利用登记处获取关于社区目前存档的组织及其访问的信息， 并促进寻求验证动物模型与人类相关性的研究人员之间的合作。 具体目标2)优化组织制备技术。我们将包括分子和细胞标记 以及可视化技术，允许直接比较新鲜和存档的人体组织与来自 动物模型。我们将发布人耳的采购、保存和制备方案 来自颞骨的组织。通过这样做，我们将鼓励动物研究人员将人类组织纳入 他们的分析。具体目标3)我们将开发工具，以开发新的技术和方法来成像和 在原地可视化人类的中耳和内耳。成像技术的目标将包括软细胞组织， 膜和液体，以及硬骨组织。具体目标4)我们将提供以下技术指导 将为调查人员、技术人员、耳鼻咽喉科住院医生、医学院和 对内耳生物学感兴趣的研究生所需的颞骨处理技术和技能 用于处理从颞骨中提取的人耳组织。这份提案中产生的材料将非常 对美国及全球基础内耳研究领域的研究具有一定的参考价值。

ABSTRACT In the present proposal we will preserve, process and make available human ear tissue to inner ear basic researchers they will be able to compare with their animal models. The use of celloidin, microdissected tissue for immunohistochemical and quantitative techniques will be instrumental in driving basic animal inner ear research into the understanding of human ear pathology and has high potential for the development of effective drug therapies for restoring auditory and vestibular function. In Specific Aim 1) we will improve access for the auditory and vestibular research community to human ear tissues from temporal bone specimens. We will improve and promote timely procurement, preparing and processing of new specimens, and appropriate re- processing of archived specimens. We will distribute a limited number of well-prepared laboratory tissues to funded researchers who lack adequate access to facilities for human temporal bone preparation. We will also exploit use of the Registry for information about currently archived tissues and their access by the community, and promote collaborations between researchers seeking to validate animal models for human relevance. Specific Aim 2) We will optimize tissue preparation techniques. We will include molecular and cellular marking and visualization techniques that allow direct comparisons of fresh and archived human tissues with those from animal models. We will disseminate protocols for procurement, preservation and preparation of human ear tissues from temporal bones. By doing this we will encourage animal researchers to include human tissues into their analyses. Specific Aim 3) We will develop tools to exploit new technologies and methods to image and visualize human middle and inner ears in situ. Targets for imaging techniques will include soft cellular tissues, membranes and fluids, as well as hard bony tissues. Specific Aim 4) We will provide technical instruction with hands-on training will be available for investigators, technicians otolaryngology residents, medical school and graduate students interested in inner ear biology in the temporal bone processing techniques and skills needed for processing human ear tissues from temporal bones. The materials generate in this proposal will be extremely useful for the research in basis inner ear research field, in the USA and around the globe.