Advanced Confocal Microscope in a multi-user facility

项目摘要/摘要 此应用程序是来自分析成像设备(AIF)的共享仪器授权，地址为 阿尔伯特·爱因斯坦医学院将获得一种新的先进的点扫描共焦 显微镜。AIF通过允许NIH资助的调查人员访问最新情况来支持他们 增强前沿、协作性和多学科的艺术显微技术 研究。目前，AIF有几种共焦显微镜可用：两个点扫描 共焦、旋转圆盘共焦和行扫描共焦。然而，四个人中有三个 现有的共焦显微镜由于老化、停止使用 供应商签订的服务合同，以及难以获得维修所需的部件。其中之一 剩余的点扫描共聚焦显微镜将无法支持目前的70多个实验室 在爱因斯坦使用共焦技术。为了继续支持正在进行的NIH资助的 生物医学研究依赖共焦显微镜，我们需要取代失败的 配备一台新的共焦显微镜的仪器，可以提供我们目前的所有功能 显微镜，包括常规的多通道高分辨率3D成像，FAST活细胞成像 帧速率和通道设置的灵活性，以支持使用多种不同的荧光团。在……里面 此外，自2007年收购我们上一家共焦公司以来，有一些主要的 共焦显微镜领域在探测器、激光器设计和 收购速度，我们相信技术的更新将使我们的一些NIH- 资助研究人员进行以前很难进行的实验。 总体而言，收购这一先进仪器将对NIH资助的 爱因斯坦的生物医学研究，包括以下主要用户项目： 果蝇的发育生物学和遗传学(贝克博士)，中枢性炎症研究 人类全身性衰老的调节(蔡博士)，癌症转移、侵袭和 血管内凝血(Condeelis博士)，线粒体功能和有丝分裂(Kitsis博士)， 糖尿病背景下的自噬和程序性细胞死亡(Pessin博士)， 原代海马神经元和组织的信使核糖核酸调控(辛格博士) 大脑和骨骼中的缝隙连接结构动力学(斯普莱姆博士)还有其他的 目前在爱因斯坦使用共焦显微镜的小型用户项目和70多个实验室，将 从这项技术中获益。

PROJECT SUMMARY/ABSTRACT This application is a shared instrumentation grant from the Analytical Imaging Facility (AIF) at the Albert Einstein College of Medicine to acquire a new, advanced point-scanning confocal microscope. The AIF supports NIH-funded investigators by giving them access to state-of-the- art microscopy technologies that enhance cutting edge, collaborative, and multidisciplinary research. Currently, the AIF has several confocal microscopes available: two point-scanning confocals, a spinning disk confocal and a line scanning confocal. However, three out of the four existing confocal microscopes are reaching their end of usage due to aging, discontinuation of service contracts by the vendors, and difficulties obtaining parts needed for repair. The one remaining point-scanning confocal microscope will not be able to support the 70+ labs currently using confocal technology at Einstein. In order to continue to support the ongoing NIH funded biomedical research that relies on confocal microscopy, we need to replace the failing instruments with one new confocal microscope that can offer all the functions of our current microscopes, including regular multi-channel high resolution 3D imaging, live-cell imaging at fast frame rates, and flexibility in channel set-up to enable use of many different fluorophores. In addition, since the acquisition of our last confocal in 2007, there have been some major innovations in the field of confocal microscopy with regard to detectors, laser design and acquisition speed, and we believe that an update in technology will enable some of our NIH- funded researchers to conduct experiments that have previously been challenging to conduct. Overall, acquisition of this advanced instrument will have a high impact on the NIH-funded biomedical research at Einstein, including the following major user projects: studies of the developmental biology and genetics of Drosophila (Dr. Baker), studies of central inflammatory regulation of systemic aging in humans (Dr. Cai), studies of cancer metastasis, invasion and intravasation (Dr. Condeelis), mitochondrial function and mitophagy (Dr. Kitsis), the regulation of autophagy and programmed cell death in the context of diabetes (Dr. Pessin), mechanisms of mRNA regulation in primary hippocampal neurons and tissues (Dr. Singer) and investigation of gap junction structural dynamics in the brain and bone (Dr. Spray). There are also additional minor user projects and 70+ labs currently using confocal microscopy here at Einstein that will benefit from this technology.