Van Breemen: Core 1; Botanical Integrity Core (Pauli)

根据NCCAM/NIH关于天然产品完整性和良好研究实践的政策，核心B将确保 该中心主要研究材料的植物学真实性和植物化学可靠性：生草药， 植物提取物和纯化的标准物质(植物化学物质)。核心B是与2010年 我们中心的资金周期，并引入了植物完整性档案(BPID)，以确保可追溯性和 学习材料的真实性。核心B将继续履行其作为植物完整性核心的使命 为整个中心提供必要的专业知识，以确保 研究材料，并将维持和扩大BID的收集。最重要的目标是获得， 生产对项目1-3中的研究至关重要的材料，并证明其生药学质量 核心C和D。核心B有三个具体目标：获取原始草药原料，鉴定它们，以及 在BID中记录它们的完整性(目标1)；生产、获取和表征最先进的植物学 提取和生成BPID(目标2)；向中心提供经过充分表征的纯化的参考材料 植物化学物质(目标3)。为此，Core B将利用现有的和现代的生药学 方法(感官、显微镜、DNA条形码)、药典、行业标准和自动化 提取程序(渗滤、液-液分配、ASE)和最新的植物化学方法： 制备层析，包括靶向逆流分离(CS)、分析(UHPLC)和 波谱技术(MS、核磁共振、Q核磁共振、HIFSA)。核心B将与分析核心D密切合作 关于LC-MS和核磁共振的分析。总体而言，核心B为可重现性提供了重要基础 以及该中心所有化学、生物和临床研究的重要性。

In line with NCCAM/NIH's Policy on Natural Product Integrity and good research practices, Core B will ensure the botanical authenticity and phytochemical reliability of the Center's key study materials: crude herbals, botanical extracts, and purified reference materials (phytochemicals). Core B was established with the 2010 funding cycle of our Center and introduced the Botanical Integrity Dossiers (BPIDs) to ensure traceability and authenticity of the study materials. Core B will continue to fulfill its mission as the Botanical Integrity Core by providing the entire Center with the essential expertise required to ensure the pharmacognostic integrity of the study materials, and will maintain and expand the collection of BPIDs. The overarching goal is to acquire, produce, and certify the pharmacognostic quality of, materials that are essential for the studies in Projects 1-3 and Cores C+D. Core B has three Specific Aims: acquire crude herbal materials, authenticate them, and document their integrity within BPIDs (Aim 1); produce, acquire, and characterize state-of-the-art botanical extracts and generate BPIDs (Aim 2); provide the Center with well-characterized reference materials of purified phytochemicals (Aim 3). For this purpose, Core B will utilize established and modern pharmacognostic methods (organoleptic, microscopy, DNA barcoding), pharmacopoeial, industry-standard and automated extraction procedures (percolation, liquid-liquid partitioning, ASE), and state-of-the art phytochemical methods: preparative chromatography including targeted countercurrent separation (CS), analytical (UHPLC), and spectroscopic techniques (MS, NMR, qNMR, HiFSA). Core B will collaborate closely with the Analytical Core D on the LC-MS and NMR analyses. Collectively, Core B provides an important foundation for the reproducibility and significance of all chemical, biological, and clinical studies in the Center.