Feature Selectivity and Interneuron Connectivity Principles of Clonally-Related Excitatory Neurons in Mouse Primary Visual Cortex

小鼠初级视觉皮层克隆相关兴奋性神经元的特征选择性和中间神经元连接原理

• 批准号: 9377506
• 负责人: Paul Gerrard Fahey
• 金额: $ 4.44万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-20 至 2019-09-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9377506
• 关键词: Address; Adult; Autistic Disorder; Brain; Calcium; Cells; Cognition; Complex; Cortical Column; Development; Dimensions; Electrophysiology (science); Exhibits; Frequencies; Hippocampus (Brain); Image; Income; Individual; Interneurons; Location; Maintenance; Measures; Morphology; Mus; Neocortex; Neurons; Output; Pattern; Perception; Photons; Play; Process; Property; Recording of previous events; Research; Role; Schizophrenia; Sensory; Surface; Synapses; System; Testing; Visual; Visual Cortex; Work; area striata; brain abnormalities; brain research; excitatory neuron; experimental study; hippocampal pyramidal neuron; improved; in vivo calcium imaging; inhibitory neuron; insight; multi-photon; neocortical; neuropsychiatry; novel; receptive field; sensory input; synaptogenesis

7. PROJECT SUMMARY/ABSTRACT Perception, cognition, and other high level brain functions are thought to arise in the neocortex, a laminar sheet of neurons forming the surface of the brain. However, the billions of neurons and trillions of synaptic connections in the cortex form an overwhelmingly complex system, and therefore more insight into the organization of the cortex at the local circuit level is needed. Previous research has suggested that ontogenetic columns (columns of clonally-related neurons) play a critical role in excitatory neuron integration in local microcircuitry, and may represent a fundamental module of cortical computation. However, the functional relationships within an ontogenetic column and the principles of ontogenetic column integration into the local circuit of inhibitory interneurons have not been examined. This project will use multi-photon in vivo calcium imaging to characterize the visual feature selectivity of clonally-related neurons across all layers of primary visual cortex, and examine the distribution of feature selectivity within a layer, and how that distribution changes between cortical layers. Additionally, using octuple simultaneous whole cell patching, this project will test different classes of cortical inhibitory interneurons for patterns of connectivity to ontogenetic columns, in order to gain insight into how clonally related neurons integrate with unrelated neurons in the local circuit. Together, these experiments will delineate the influence of ontogenetic history on the functional organization and microcircuitry of adult neocortex. The results of this work have the potential to reveal fundamental new insights into how the brain processes incoming sensory information, and how that information is transformed as it passes from one cortical layer to another. These results will thus enable a more principled understanding of normal and abnormal circuit function, with broad implications for a wide range of currently intractable neuropsychiatric illnesses such as autism and schizophrenia.

7.项目摘要/摘要 知觉、认知和其他高级大脑功能被认为出现在新皮质，一种 构成大脑表面的神经元的片状结构。然而，数十亿个神经元和数万亿个 皮质中的突触连接形成了一个压倒性的复杂系统，因此更深入地了解 需要在局部电路级别上组织皮质。此前的研究表明， 个体发生柱(克隆相关神经元的柱)在兴奋性神经元整合中起着关键作用 在局部微电路中，并且可能代表大脑皮层计算的基本模块。然而， 个体发育柱内的功能关系及个体发育柱整合原理 进入局部回路的抑制性中间神经元还没有被研究过。 该项目将使用多光子活体钙成像来表征视觉特征的选择性 克隆相关神经元在初级视皮层的所有层，并检查特征的分布 一层内的选择性，以及这种分布在皮质层之间的变化。此外，使用 八个同步的全细胞贴片，这个项目将测试不同类别的皮质抑制 中间神经元连接到个体发育柱的模式，以了解克隆是如何 相关神经元与局部回路中的无关神经元整合在一起。总而言之，这些实验将 个体发育史对成人功能组织和微回路的影响 大脑皮层。这项工作的结果有可能揭示大脑是如何 处理传入的感官信息，以及当信息从一个感官信息传递到 皮质层到另一层。因此，这些结果将使我们能够更原则性地理解正常和 电路功能异常，对目前难以治愈的一系列神经精神疾病具有广泛的影响 自闭症和精神分裂症等疾病。