Borrelia burgdorferi responses to antibiotic stresses

伯氏疏螺旋体对抗生素应激的反应

• 批准号: 9374989
• 负责人: Brian Stevenson
• 金额: $ 8.96万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-06 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9374989
• 关键词: Address; Animals; Antibiotics; Bacteria; Borrelia burgdorferi; Development; Enzymes; Escherichia coli; Exhibits; Gene Expression; Genes; Genetic Transcription; Human; Individual; Infection; Laboratory Animals; Laboratory Study; Lyme Disease; Metabolic; Molecular; Order Spirochaetales; Patients; Physiological Adaptation; Production; Proteins; Proteome; Research Personnel; Residual state; Staphylococcus aureus; Stress; Symptoms; Therapeutic; Ticks; Tissues; base; improved; insight; killings; pathogen; pathogenic bacteria; response; transcriptome

ABSTRACT Lyme disease, which is caused by the spirochete Borrelia burgdorferi, is generally treatable by a 2-4 week course of antibiotics. However, there is strong evidence from studies of human patients and experimental animals that antibiotics do not always clear B. burgdorferi infections. Studies of other bacterial species have revealed genetically-encoded physiological adaptations to antibiotic stresses, such as altering expression levels of target proteins or metabolic enzymes. To gain insight on whether the Lyme spirochete also adapts to antibiotic stresses, we will examine the transcriptomes and proteomes of B. burgdorferi that are exposed to sub-lethal and lethal concentrations of antibiotics that are commonly used to treat human Lyme disease infections. Results of these studies will reveal whether B. burgdorferi possesses genetically-encoded adaptive mechanisms, which can then be targeted for development of improved therapeutics.

抽象的 莱姆病是由螺旋藻伯氏伯氏菌引起的，通常可以通过2-4周来治疗 抗生素的过程。但是，人类患者和实验的研究有强有力的证据 抗生素的动物并不总是清除B. burgdorferi感染。对其他细菌物种的研究 揭示了遗传编码对抗生素应激的生理适应，例如改变表达 靶蛋白或代谢酶的水平。要了解莱姆螺旋体是否也适应 抗生素应力，我们将检查暴露于Burgdorferi的转录组和蛋白质组织 通常用于治疗人莱姆病的抗生素的亚致死和致命浓度 感染。这些研究的结果将揭示B. burgdorferi是否具有遗传编码的适应性 然后，机制可以针对改善治疗剂的发展。