RESOURCE/SERVICE CORE A - OCULAR IMAGING MODULE

资源/服务核心 A - 眼部成像模块

• 批准号: 9336309
• 负责人: William Joseph Dupps
• 金额: $ 33.85万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9336309
• 关键词: Angiography; Animal Model; Animals; Anterior; Center Core Grants; Clinic; Collaborations; Core Grant; Cornea; Cryoultramicrotomy; Darkness; Data; Diagnostic; Disease Progression; Eye; Faculty; Fluorescence; Fostering; Foundations; Frozen Sections; Funding; Fundus; Fundus photography; Generations; Glaucoma; Goals; Grant; Image; Imaging Techniques; Imaging technology; Immunohistochemistry; Lasers; Mind; Modeling; Mus; Operative Surgical Procedures; Ophthalmology; Ophthalmoscopy; Optical Coherence Tomography; Oryctolagus cuniculus; Paraffin; Paraffin Embedding; Plastic Embedding; Plasticizers; Process; Productivity; Protocols documentation; Rattus; Recruitment Activity; Research; Research Personnel; Research Support; Resources; Retinal Diseases; Risk; Scanning; Scientist; Services; Staining method; Stains; Standardization; Supervision; Talents; Techniques; Tissue Embedding; Tissue Fixation; Tissue imaging; Transmission Electron Microscopy; Vision; Vision research; Visual system structure; Zebrafish; animal imaging; cellular imaging; ex vivo imaging; experience; faculty research; imaging modality; innovation; microscopic imaging; programs; ranpirnase; sample fixation; tissue processing; treatment planning

Abstract Ocular Imaging Module The Ocular Imaging Module will provide a variety of imaging modalities to the investigators involved in this P30 Core Grant for Vision Research. The module includes live animal (in vivo) imaging with slit lamp and fundus photography, scanning laser ophthalmoscopy (SLO) and optical coherence tomography (OCT). It also supports tissue and cellular imaging (ex vivo) support, providing routine and specialized tissue fixation techniques, paraffin and plastic tissue embedding, sectioning (paraffin and Cryo) and staining for immunohistochemistry. Microscope imaging techniques include bright field, dark field, differential interference contrast (DIC), fluorescence, and transmission electron microscopy (TEM). It also provides help and advice with the morphometric analysis of generated images. Access to this core module will support NEI R01 funded investigators, help newly recruited investigators obtain data for NEI R01 grant submissions, foster collaborative interactions between RO1 funded investigators and newly recruited investigators, attract investigators from other departments to research on the visual system and be a valuable departmental resource in attracting additional vision scientists to our program as we expand our research faculty.

摘要 眼科成像模块 眼科成像模块将提供各种成像方式 参与这项P30视觉研究核心拨款的调查人员。该模块包括 活体动物(活体)用裂隙灯和眼底照相术、扫描激光成像 眼底镜(SLO)和光学相干断层扫描(OCT)。它还支持 组织和细胞成像(体外)支持，提供常规和专门的组织 固定技术，石蜡和塑料组织包埋，切片(石蜡和冷冻) 免疫组织化学染色。显微镜成像技术包括BREIGH 场、暗场、差分干涉对比度(DIC)、荧光和透射率 电子显微镜(TEM)。它还提供有关形态测量的帮助和建议 分析生成的图像。访问此核心模块将支持由NEI R01资助 调查人员，帮助新招聘的调查人员获得NEI R01拨款的数据 提交，促进RO1资助的调查人员和 新招聘的调查员，吸引其他部门的调查员进行研究 视觉系统，并成为吸引更多视力的宝贵部门资源 科学家加入我们的项目，因为我们扩大了我们的研究人员队伍。